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This article discusses an alternative approach by creating a Custom Field to calculate resolution between the two peaks. This would be a Peak Custom Field using Intersample syntax.

STEP 3 The calculation for USP Resolution = 2(RT2-RT1)/(W1+W2), where RT is the ‘retention time’ and W is the ‘width at Tangent’ for USP Resolution (figure 3).

STEP 2 Be sure to enable System Suitability in the Processing Method as we will need the ‘Width at Tangent for USP Resolution’ values for our Custom Field (figure 2).

STEP 1 We have a stack plot of the two channels of data, each with its own peak eluting at different retention times (figure 1).

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STEP 4 Intersample syntax is ‘label.injection.channel (field). (Refer to Empower Tip #93 for more details on creating intersample Custom Fields) (figure 4).

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Highway Stars, Ltd. is a participant in the GM Restoration Parts license program. Products using this GM Restoration emblem are licensed products accepted in the GM licensing program. The GM Restoration Parts emblem/logo is a registered trademark of General Motors and used under license to Highway Stars, Ltd. Only products bearing the GM restoration emblem have been accepted into the program.

id226694, baseline ripple, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

STEP 6 The result displaying the resolution between the two peaks in the two different channels is different from the value calculated in Tip #260. The baseline in the combined chromatogram is different from the baselines in the individual chromatograms resulting in different baseline noise, different peak starts/stops and different peak widths. The key is to pick one approach for consistency in the calculations (figure 6).

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id226694, baseline ripple, EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP