You can increase or decrease the size of the aperture that the light passes through using a simple lever on the side of the condenser.

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These essential components play a critical role in the quality of your images – and you’ll need to understand a little of their nuances to get a sharp, well-contrasted look at your samples.

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If you’re interested in making the most of your microscope, you’ll want to get to grips with the condenser and aperture diaphragm on your scope.

Collectively, they determine resolution, contrast and depth of field. The position of that little lever decides whether you’ll see a washed-out mess or a crisp, detailed specimen.

The condenser is a lens or series of lenses that usually sits just below the microscope stage. Its role is to focus the light into an even illumination to give a clear and undistorted view of your samples.

The aperture diaphragm, also known as the iris or iris diaphragm, consists of a circle of interlocking petals or blades that sits within the condenser. It functions a bit like curtains, controlling the amount of light that gets through the condenser.

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Many novice users are tempted to close the iris and crank up the illuminator setting for a bright, yet highly contrasted image. But hold on a sec!

CS Mount Adapter

But note that you can’t increase the resolution beyond the NA value, so opening the diaphragm any further won’t give you any additional benefits.

CS-Mount lens

Everyone’s preferences and specimens are different, so don’t be afraid to play around with the settings until you find that sweet spot!

While it sounds like they simply act as another illuminator control or a glorified dimmer switch, they actually have a massive impact on the quality of your images.

Closing the diaphragm will increase the contrast and depth of field, but at the expense of resolution and brightness. Opening it up will do the opposite.

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If you’re looking to maximise resolution, match the aperture setting (the numbers below the control lever) to the numerical aperture (NA) value of the objective lens you’re using.

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You can’t have it all, so it’s about finding the right balance for the specimen at hand. A rule of thumb is to have the diaphragm 50 to 90% open for a good mix of resolution and contrast.

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While it can be helpful, it can also introduce a few optical issues. Dust and debris on the cover slip or optical surfaces can become more visible and obstruct the view, and specimen structures can overlap or become hidden.