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Dividing forms of T. brucei (A) and ring form of P. falciparum (B) in peripheral blood film, ×1000 magnification. Corticated A. lumbricoides ovum (C) and T. trichiura ovum (D) in stool, ×400 magnification. A variety of staining techniques are shown.

Hold the camera lens against the microscope eyepiece. A rubber cup over the eyepiece helps to hold the camera steady. A small circle of light will be seen on the camera's LCD screen.

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Articles from The American Journal of Tropical Medicine and Hygiene are provided here courtesy of The American Society of Tropical Medicine and Hygiene

Obtaining images of clinical specimens viewed by a microscope can be invaluable for both diagnosis and teaching. Photography by a microscope normally requires a specially adapted microscope with a camera port, a camera with removable lens system, and an adapter to attach the camera to the port.1 This equipment is prohibitively expensive for many laboratories, especially in the developing world. The advent of affordable compact digital cameras with displays that reflect precisely the image as seen through the camera lens has made possible a simplified method of taking photographs through a microscope. Almost any combination of light microscope and compact digital camera with optical zoom (including some camera phones) can be used without the need for specialized equipment. Images obtained can be transmitted instantaneously by e-mail or mobile phone picture message for further timely examination by a specialist at another location (e.g., to check species identification of a parasite). This technique therefore has great potential to enhance clinical care in resource poor settings, including in remote areas. It also enables the accumulation of a library of locally relevant clinical images for use in teaching laboratory staff and clinicians and for documentation in research.

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We describe a simple, economical, and highly practical technique for taking digital photographs of specimens visualized through a light microscope. Most models of light microscope and compact digital camera, and even some cameraphones, can be used. The technique is quick to learn and can easily be performed in a resource-poor setting. It can be used to assist with diagnosis in remote areas and can be extremely useful for teaching.

For still photography and relatively short exposures you absolutely do not need an actual ND filter to get the ND effect. You can use median ...

Authors' address: Richard J. Maude, Gavin C. K. W. Koh, and Kamolrat Silamut, Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, 3rd Floor, 60th Anniversary Chalermprakiat Building, 420/6 Ratchawithi Rd., Ratchathewi District, Bangkok 10400, Thailand, Tel: +66-2-203-6333, Fax: +66-2-354-9169.

*Address correspondence to Richard J. Maude, Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine Mahidol University, 3rd Floor, 60th Anniversary Chalermprakiat Building, 420/6 Ratchawithi Road, Ratchathewi District, Bangkok 10400, Thailand. E-mail: richardmaude@gmail.com

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Group Delay Dispersion (GDD). The group delay dispersion is the change in the group delay depending on the frequency of the propagating light. Since a pulse ...

Applications of Polarization of Light · In the production of glare-reducing sunglasses. · In many industries, polaroid filters are used for stress analysis tests ...

If the image is too dark or grainy, the camera's ISO setting should be increased (usually 100 or 200 will suffice) or the “darkness” or “night time” setting selected, depending on the model of camera. Note that generally, the higher the ISO, the more difficult it is to obtain a clear image.

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While holding the camera very still, a photograph can be taken, and the image can be examined to see if it is satisfactory. Excessive blur from camera shake can be minimized using a remote control, by attaching the camera to the microscope using sticky tape or bungee cords or by constructing a frame to hold the camera in place.

Jan 9, 2022 — How to Use the Lens Equation to Find the Focal Length of a Lens. Step 1: Determine whether the object distance is positive or negative. Step 2: ...

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This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The minimum equipment required for this technique is a microscope with eyepiece and a compact digital camera or cameraphone with auto focus, through-the-lens light meter, minimum ×3 optical zoom, and LCD screen and a microscope with eyepiece. The technique is as follows:

Use the camera's zoom function to increase the size of the circle as required. The most difficult step is moving the camera lens small distances across the eyepiece to center the circle. The camera's autofocus should then self-adjust to give a clear image.

Reprint requests: Richard J. Maude, Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine Mahidol University, 3rd Floor, 60th Anniversary Chalermprakiat Building, 420/6 Ratchawithi Road, Ratchathewi District, Bangkok 10400, Thailand, Tel: +66-2-203-6333, Fax: +66-2-354-9169, E-mail: richardmaude@gmail.com.

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