Jun 11, 2018 — Overexposure to UV radiation leads to sunburn in the short-term, which is painful and, if severe enough, can be considered a first-degree burn.

Carlsson, K. The influence of specimen refractive-index, detector signal integration, and nonuniform scan speed on the imaging properties in confocal microscopy. J Microsc.-Oxford 163, 167–178 (1991).

Sphericalaberration formula

Sheppard, C. J., Gu, M., Brain, K. & Zhou, H. Influence of spherical aberration on axial imaging of confocal reflection microscopy. Appl. Opt. 33, 616–624 (1994).

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Sphericalaberration in mirrors

Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I. & North, A. J. Tutorial: guidance for quantitative confocal microscopy. Nat. Protoc. 15, 1585–1611 (2020).

Hell, S., Reiner, G., Cremer, C. & Stelzer, E. H. K. J. Microsc. 169, 391–405, (1993): https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2818.1993.tb03315.x

Sphericalaberration and chromatic aberration

Visser, T. D. et al. Optik 90, 17–19 (1992): https://www.researchgate.net/publication/285251956_Refractive_index_and_axial_distance_measurements_in_3-D_microscopy

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Zhang, Q. et al. Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging. Sci. Rep. 7, 2532 (2017).

Sphericalaberration photography

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Heine, J. et al. Three dimensional live-cell STED microscopy at increased depth using a water immersion objective. Rev. Sci. Instrum. 89, 053701 (2018).

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What issphericalaberration in Physics

Sphericalaberration in a lens

Hell, S., Reiner, G., Cremer, C. & Stelzer, E. H. K. Aberrations in confocal fluorescence microscopy induced by mismatches in refractive-index. J. Microsc. 169, 391–405 (1993).

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Ghosh, S. & Preza, C. Three-dimensional block-based restoration integrated with wide-field fluorescence microscopy for the investigation of thick specimens with spatially variant refractive index. J. Biomed. Opt. 21, 46010 (2016).

by EJ Hysom · 1966 · Cited by 1 — ... dielectric mirrors, which have iOO~? reflectivity, for astronomical ... In the case, 0±' the all—reflecting dielectric mirror, what is done is to ...

Patwary, N., King, S. V., Saavedra, G. & Preza, C. Reducing effects of aberration in 3D fluorescence imaging using wavefront coding with a radially symmetric phase mask. Opt. Express 24, 12905–12921 (2016).

Keller, H. E. Objectives for confocal microscopy. in Handbook of Biological Confocal Microscopy (ed Pawley, J. B.) (Springer, 1995).

Sphericalaberration example

Engelbrecht, C. J. & Stelzer, E. H. Resolution enhancement in a light-sheet-based microscope (SPIM). Opt. Lett. 31, 1477–1479 (2006).

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Renier, N. et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896–910 (2014).

Peer review information Nature Protocols thanks Chrysanthe Preza and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012).

We thank S. Piccinotti and L. Rubin for providing organoid samples. We thank the Harvard Center for Biological Imaging for infrastructure and support. J.W.L. was supported by the following funding sources: National Institutes of Health grants P50 MH094271, U24 NS109102, and U19 NS104653 and Department of Defense MURI award GG008784.

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Preza, C. & Conchello, J. A. Depth-variant maximum-likelihood restoration for three-dimensional fluorescence microscopy. J. Opt. Soc. Am. A 21, 1593–1601 (2004).

Gibson, S. F. & Lanni, F. Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy. J. Opt. Soc. Am. A 9, 154–166 (1992).

Model, M. A., Fang, J., Yuvaraj, P., Chen, Y. & Zhang Newby, B. M. 3D deconvolution of spherically aberrated images using commercial software. J. Microsc. 241, 94–100 (2011).

Sphericalaberration correction

Ghosh, S. & Preza, C. Fluorescence microscopy point spread function model accounting for aberrations due to refractive index variability within a specimen. J. Biomed. Opt. 20, 75003 (2015).

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Diel, E.E., Lichtman, J.W. & Richardson, D.S. Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy. Nat Protoc 15, 2773–2784 (2020). https://doi.org/10.1038/s41596-020-0360-2

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Another way to make a diffraction grating is to start with a coherent laser. Expand the beam to your desired size and then split it into two ...

Kim, B. & Naemura, T. Blind depth-variant deconvolution of 3D data in wide-field fluorescence microscopy. Sci. Rep. 5, 9894 (2015).

All necessary code and instructions for running the axial correction macro are provided in the Supplementary Software and Box 1.

The idea for calculating axial distortion correction factors as described in this tutorial was conceived by D.S.R. and J.W.L. E.E.D. and D.S.R. carried out experiments and analyzed data. D.S.R., J.W.L. and E.E.D. wrote the manuscript. All authors contributed to editing the final manuscript.

Schmidt, N. C., Kahms, M., Huve, J. & Klingauf, J. Intrinsic refractive index matched 3D dSTORM with two objectives: comparison of detection techniques. Sci. Rep. 8, 13343 (2018).

Spherical aberration (SA) occurs when light rays entering at different points of a spherical lens are not focused to the same point of the optical axis. SA that occurs inside the lens elements of a fluorescence microscope is well understood and corrected for. However, SA is also induced when light passes through an interface of refractive index (RI)-mismatched substances (i.e., a discrepancy between the RI of the immersion medium and the RI of the sample). SA due to RI mismatches has many deleterious effects on imaging. Perhaps most important for 3D imaging is that the distance the image plane moves in a sample is not equivalent to the distance traveled by an objective (or stage) during z-stack acquisition. This non-uniform translation along the z axis gives rise to artifactually elongated images (if the objective is immersed in a medium with a higher RI than that of the sample) or compressed images (if the objective is immersed in a medium with a lower RI than that of the sample) and alters the optimal axial sampling rate. In this tutorial, we describe why this distortion occurs, how it impacts quantitative measurements and axial resolution, and what can be done to avoid SA and thereby prevent distorted images. In addition, this tutorial aims to better inform researchers of how to correct RI mismatch–induced axial distortions and provides a practical ImageJ/Fiji-based tool to reduce the prevalence of volumetric measurement errors and lost axial resolution.

Visser, T. D., Oud, J. L. & Brakenhoff, G. J. Refractive-index and axial distance measurements in 3-D microscopy. Optik 90, 17–19 (1992).