ND Filter - what is a neutral density filter
Inherited, incurable condition that causes nerve cells in the brain to break down over time, causing jerky body movements and eventually the inability to talk.
In stationary microscopes, the objective lens then focuses reflected light from the object up a tube toward the ocular lens, which is the lens the user looks ...
A genetically encoded calcium indicator used in brain imaging. GCaMP is similar to the fusion of green fluorescent protein (GFP), calmodulin, and a peptide sequence from myosin.
Fluorescence imaging is a powerful, highly sensitive and non-invasive technique used in life sciences to visualize and monitor biological processes in live or fixed cells, tissues, or even complete organisms. Fluorescence is the emission of radiation, visible or invisible, by a substance such as a fluorescent dye (also called fluorophores or chromophores), upon excitation with light or other electromagnetic radiation. There are a wide range of fluorescent dyes and proteins commercially available that can be used to label biological structures with high specificity. Fluorescence imaging techniques can be divided into spectroscopic and microscopic fluorescence methods. Applications based on spectral detection include qPCR, DNA sequencing, high-throughput screening, or flow cytometry. Fluorescence microscopy techniques commonly used in biomedical research laboratories include epifluorescence, confocal, multiphoton, TIRF (total internal reflectance fluorescence), super-resolution (SIM, STORM, PALM, STED), or light sheet microscopy, as well as fluorescence lifetime imaging. The most suitable technique will depend on multiple factors including the level of two- or three-dimensional resolution, imaging speed, imaging depth, or the number of colour channels required, but all utilize the same fluorescence mechanism to observe biological processes.
Many optical diagnostic techniques are used to examine, diagnose, and treat medical condiditons for example of the brain including laser-based microscopy, and optogenetics.
Severe inflammation of the brain and spinal cord membranes that is typically onset by an infection and leads to fever, headache, and neck stiffness.
In spherical mirrors and thin lenses, the focal length shares a direct relationship with the radius of curvature. A lens with a larger radius of curvature has a ...
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Infinity Corrected Objectives are microscope objectives that focus at infinity. They offer long working distances, along with the ability to place optical components, such as optical lenses or optical filters, into the optical path. Infinity Corrected Objectives feature higher resolutions than Finite Conjugate Objectives, but are heavier and more expensive.
Finite Conjugate Objectives are microscope objectives that do not require a secondary lens to focus onto a specimen. They are typically optimized for use in the visible spectrum and contain up to four lens elements.
Method of making brain tissue transparent using hydrogels. Accompanied with antibodies or biomarkers, highly detailed pictures of nucleic structure of the brain can be determined and studied.
Below are common ailments of the brain that are detected by advanced diagnostic techniques such as fluorescence microscopy. Advancements in microscope objectives and other optical components allow these ailments to be more easily detected and treated.
Problem 1 – The table below gives the optical data for some large telescopes. Use this data to calculate the magnification for each indicated lens. Also fill in ...
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Electrophysiology technique allowing the study of single and multiple ion channels in neurons, cardiomyocytes, muscle fibers, and other cells.
Neuroscience technique intended to map and list out the specific quantities or properties of the brain in a spatial representation. In other terms, the anatomy and function of the brain, spine, and central nervous system through imaging techniques.
The ocular lenses are the lenses closest to the eye and usually have a 10x magnification. Since light microscopes use binocular lenses there is a lens for each ...
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Reflective Objectives are microscope objectives that use two mirrors to form an image or focus a laser in microscopy applications requiring chromatic correction over broad spectral ranges.
Transfeminine (also written trans-feminine or trans feminine, sometimes abbreviated to transfem or transfemme[note 1], and sometimes known as cross-feminine or cross-femme[citation needed]) describes a person, transgender or otherwise (generally but not exclusively one who was assigned male at birth) who seeks to present femininely, identifies as more female than male, or wishes to transition to look more feminine. In general, although not exclusively, the prefix "cross-" is used by individuals who do not wish to nor believe themselves to be in transition, but still does not align their life-style or gender choice to be in full or partial synchrony with their physical sex.[citation needed]
Some neutrois people [neutrois is a specific nonbinary identity that is neither male nor female] feel they aren’t completely 100% gender-free or gender-neutral; rather, they lean a little more towards one side or another of the gender spectrum. Transfeminine means the person tilts towards female [...] It’s important to note this does not invalidate, contradict, or cancel out being neutrois, as they still feel a strong affinity with this identity. Instead, being transmasculine [...] is more of a modifier or a complement which adds to the complexity of their gender, gender expression, or gender identity. In these cases there might be a preference to present more closely to one gender over another, or it can be more comfortable to just live as one binary gender rather than the other. However, this choice is more often a result of convenience in order to navigate a society in which only two genders are recognized. A lot of people would ideally opt to have neutrois recognized as their gender and not be forced to make a decision between male and female only.
Green fluorescent protein (GFP) is a specialized protein consisting of a specific group of amino acids that glows green when exposed to UV or blue light. Extracted from marine life, the most common excitation wavelength is 395nm to 475nm with emission peaks from 509nm to 525nm. GFP is widely used in non-invasive fluorescence imaging systems to detect tumor growth, apoptosis, and other cellular activity.
Medical condition caused when blood supply to the brain is interrupted for an extended period of time, resulting in muscle weakness on one side of the body, loss of face control, numbness, and speech issues.
The word "transfeminine" was used in a 1985 issue of The TV-TS Tapestry, a magazine "for persons interested in crossdressing & transsexualism". In that issue, Jane Nance wrote about the difficulties of describing herself with the then-current terminology: Since Jane felt her identity was fully womanly, she did not want to identify as "transvestite", and since she did not want surgical transition she felt that "transsexual" was not accurate either, and neither was "transgenderist", since "I'm living and functioning in the world most of time in the male role." She proposed "transfeminine" as a possibility and said that the definition could be "a male who feels like a female, strictly undefined in relation to any issue of an operation".[4] This might or might not be the first recorded usage of the word "transfeminine".
The meaning of DIFFRACTION GRATING is grating.
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Cancerous or non-cancerous growths of abnormal cells in the brain with a variety of severity levels and types including astrocytoma, blastoma, ependymoma, and meningioma.
The Largest Telescope Distributor on Long Island, NY You can find Astronomical cameras(CCD & CMOS) William Optics, Explore Scientific, Celestron, ...
noun · a surface, such as polished metal or glass coated with a metal film, that reflects light without diffusion and produces an image of an object placed in ...
Progressive and incurable type of dementia that destroys memory and other important mental functions, beginning slowly and worsening over time.
Apochromatic Objectives are microscope objectives that feature better color correction than Achromatic Objectives over a broad wavelength range (VIS to NIR), along with better spherical correction and field flatness. However, they are heavier and more expensive than Achromatic Objectives.
Microscopy techniques such as fluorescence, confocal, multiphoton, and super resolution microscopy are used for the study of synapses, neurons, and neural circuits in brain slices.
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by C Dwivedi · 2020 · Cited by 7 — Metals are opaque at IR wavelengths. This is because metals are characterized by their large concentrations of free electrons, which is the property that gives ...
Multi-element objectives are critical for any microscopy technique, including many diagnostic techniques for the brain. Understanding the different types of objectives is important in order to ensure you are using the correct objective for your application.
A similar but distinct umbrella term to the trans-feminine spectrum is the male-to-female spectrum (MtF spectrum), meaning that they were assigned male at birth, and transition in a more female direction. This term is also not limited to people who specifically identify as women. "Trans-feminine" and "MtF spectrum" carry different nuances of meaning that may suit people in different ways. Trans-feminine doesn't call out someone's birth assignment, but does call out their gender expression as being feminine. There are trans women who prefer a more butch than feminine gender expression. Meanwhile, MtF spectrum doesn't specify one's gender expression as being feminine, calls out one's birth assignment, but transgender people can feel uncomfortable with having their gender assignment pointed out. Due to these nuances, people may feel that one term is more suitable than the other for their own comfort and for the most accurate description of their identity.
The most common type of traumatic brain injury, onset by heavy impact resulting in the brain shaking or moving inside the skull.
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Notable people who consider their identity to be outside the Western gender binary, and who describe themselves with the word "transfeminine," include:
Fluorescence filter sets are particularly important in separating the excitation light from the emitted fluorescence and commonly consist of a combination of an excitation (typically bandpass), dichroic (sometimes referred to as dichroic mirror or dichromatic beamsplitter), and emission (bandpass or longpass) filter with transmission profiles optimized to match the spectral characteristics of certain fluorophores.
Biological technique that involves the use of light to control cells in living tissue, specifically neurons, in most cases that have been genetically modified with photoreceptors that react to different wavebands.
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Fluorescence microscopy is one of the primary techniques used to study the functional or morphological dynamics of synaptic structures, including dendritic spines and axon terminals, and to characterize circuit connectivity and dynamics. Typically, a laser beam is focused into a pinhole, acting as a point source illuminator. Spatially filtered light is reflected by a dichroic filter, which may or may not require a beam expander to fill the entire aperture of the objective. The objective then focuses excitation energy onto the sample, which emits a weaker fluorescence signal collected by the same objective. This emission light is transmitted through the dichroic filter into the secondary tube lens and then passes through a final pinhole before detection by a CCD or CMOS sensor. Ideally, both pinholes are located in conjugate image plans on the optical axis, which allows the images to overlap perfectly on the object plane. Since confocal microscopes observe a very thin, small spot in the object plane, it is important to sample light via a scanning system or motorized actuator to collect an array of images. These images are then reconstructed into 2D or 3D images.
Achromatic Objectives are microscope objectives that correct for chromatic aberration in red and blue wavelengths, and spherical aberration in the green wavelength. Achromatic Objectives are lighter and cheaper than Apochromatic Objectives, but do not have as much correction for chromatic aberration, spherical aberration, and field flatness.
So here is my rule of thumb… outline your full coverage tip before putting it on the natural nail by putting as much gel as the length of the nail bed you are ...
The labels can be considered to be a gender identity, a gender expression, or both. It is an umbrella term that includes trans women who don't consider themselves nonbinary, and nonbinary feminine people.[1][2] Some examples of genders that transfeminine individuals may identify as include:
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