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What is the main difference between single beam and double beam spectrophotometer? If the absorbance of the sample is 1.00 at a specific wavelength, what is the percentage amount of light that is absorbed by the sample and what is the percentage amount of light that is transmitted? The absorbance of a Co(ll) solution containing mg Co(Il) /mL was reported as 0.500 at 732 nm. What will be the absorbance if the solution is diluted to twice its original volume?

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Question 1: Draw an arrow on the following absorbance spectrum for FDSC Green 3 indicating its Amax value and estimate the absorbance of this dye at that wavelength. Question 2: Use Beer's Law (A = Ebc) to calculate the molar extinction coefficient (molar absorptivity; ε) of this dye at its Amax. This spectrum was collected in a cuvette with a 1.00 cm path length (b). The concentration of the dye is approximately 6.1 μM. Question 3: The molar extinction coefficient of FDGC Green 3 at 624 nm at pH 4.5 is 8.1 * 10^4 M^-1cm^-1. What concentration of the dye would give an absorbance of 0.80? Question 4: How many grams of FDGC Green 3 (809 g/mol) are required to prepare 100.0 mL of a solution of this concentration? Question 5: The analytical balance will allow you to measure as low as 20 mg of dye.

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I would like to have the answer for all 4 questions since they are related. thank you 1. If you shine a green laser beam through a green solution and you hold a piece of paper on the other side of the solution, what do you expect to see on the paper? 2. If you shine a green laser beam through a red solution and you hold a piece of paper on the other side of the solution, what do you expect to see on the paper? 3. When you double the size of the cuvette, what happens to the absorbance? Why? Explain what is happening at the molecular level. 4. When you halve the concentration of the solution, what happens to the absorbance? Why? Explain what is happening at the molecular level.

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When you shine a green laser beam through a green solution, the green solution absorbs other colors of light and transmits green light. Therefore, when you hold a piece of paper on the other side of the solution, you would expect to see a green spot on the paper.  Show more…

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