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Similar new CSC lens mounts which followed like the Sony NEX, Nikon CX, Pentax Q or EOS M mounts allow C-mount lens adaption too. Lens adaptors are widely available except for Samsung's mount.

Cine cameras were often equipped with superb C-mount optics made by renowned lens makers like Kern, Taylor-Hobson, Dallmeyer, Meyer, Angénieux, Elgeet, Berthiot, and Schneider. Wollensak and Kodak also made some fine cine lenses. Video camera C-mount lenses were made for example by Canon and Fuji. Survey lens makers and industry lens makers are Fujian, Rainbow, Computar, Tamron and Pentax (Cosmicar brand).

C-Mount adapter

The sample is throwing light out in all directions and the job of the objective is to collect as much of that light as possible. The way to do that is to have a high numerical aperture, a big wide cone. If the objective doesn’t collect a wide-angle of the cone, for example, a long working distance, low power objective will merely be getting the light that’s going straight through. That is why numerical aperture is the key to high-resolution imaging. There is one other variable that can be adjusted. By using bluer light, the resolution can be increased, but for a particular application, that may not be possible. Generally speaking, for any given objective, it is worth it to pay to get the highest possible numerical aperture, but there is a slight downside to that.

To better understand diffraction imagine if light moved strictly in straight lines. If a pinhole is illuminated with some light, the light would be directed in a straight beam. What actually happens is due to the wave nature of light, the light is diffracted, and instead of going straight, it spreads out into a cone. As shown in the diffraction image, the brightest light is the zero-order straight through, then the intensity decreases for the first order, second order, third order, etc. Unless the objective is capturing all of those higher orders, it is difficult to synthesize a high-resolution image. This describes what the fine structure of your sample is doing, it could be cells, chromosomes, or nuclei, and all of that fine structure spreads the light out.

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The consequence of numerical aperture is that it directly relates to the Depth Of Field (DOF). For a given objective, looking at a sample, there’s a particular plane of perfect focus. The depth of field is, how far above and below that plane the objective and sample can be and still have everything in focus.

An issue may be the lens' back when it is longer than its C-mount thread. For example Sony TV-lenses or Rainbow industrial process control lenses might not fit in µ4/3 cameras. Another issue are survey camera lenses for the similar CS-mount which are made for a shorter flange/sensor distance and smaller image size. On C-mount they can make only macro images which must be cropped.

C-Mount Lens holder

Meanwhile, the image circle of many lenses is unlikely to cover the entire area of the image sensor without serious vignetting since 16mm frame size is smaller than a µ4/3 sensor. Especially wide-angle C-mount lenses covering the whole sensor are very rare. The C-mount enthusiast will have to crop many images and videos shot with C-mount lens.

When it comes to digital imaging sensors, there is a wide variety to choose from. The main camera sensors used are CCD (charge-coupled device) or CMOS, Complementary Metal Oxide Semiconductor. For cutting edge performance, to reduce read noise, large and expensive cameras exist with liquid or Peltier coolers (deep cooled to about -60 degrees Celsius). Also, EMCCD (Electron Multiplied CCD) cameras allow for single photon detection and are popular for live cell imaging applications. Learn more about key aspects of image sensors common to both CCD and CMOS devices, starting at the pixel level in our "CCD Image Sensors" whitepaper.

This is called the depth of field. The formula for the depth of field is: where: “n” is the refractive index of the material between the objective and the sample λ (lambda) is the wavelength of light NA is the Numerical Aperture

In the previous example we considered a sensor with 4 micron pixels used with an objective with 40X magnification and a numerical aperture of 0.8. The sensor and magnification provide 100 nm geometric resolution. However, due to diffraction, the sample image resolution will be greater than 100 nm. For example, yellow green light has a wavelength lambda of 550 nm. Using the above equation, the diffraction limited resolution is actually 419 nm. Therefore, the resolution of the sample is > 4 times the geometric resolution at the camera sensor which results in oversampling. While some oversampling is appropriate, 4 times is excessive and a waste of sensor pixels. Calculate the diffraction using our calculator.

C-Mount Lens

Some think the older uncoated lenses may be more satisfactory for bokeh experiments, but a cheap "TV lens" for survey cameras may surprise positively too. Zoom lenses made for C-mount are a bit bulky and heavy but may be attractive for videographers who use for example a Panasonic Lumix GH µ4/3 camera since many C-mount zoomers are much faster than the original CSC zoom lenses.

In addition to affecting the observation of the specimen itself, the field of view can also impact the ability to capture high-quality images and videos. A larger field of view requires a larger camera sensor or eyepiece, which can increase the cost of the microscope system. It is essential to consider the balance between field of view, magnification, and cost when selecting and optimizing a microscope for a particular application.

For a low power 4x system, the numerical aperture is going to be very low, on the order of 0.05 to 0.1. In a medium power 40x system, it could be in the range of 0.5 to 0.8 and for a high power system, it can be as high as 0.9 or 0.95. As long as there is air between the objective and the sample, the numerical aperture can never exceed 1. When imaging slides, in order to exceed an NA of 1, a liquid can be added between the coverslip and the objective. Typically, oil or water are used for this and the objectives are referred to as oil immersion or water immersion objectives. With water, the numerical aperture goes up to about 1.1 and using oil, the numerical aperture can go up to as high as 1.47.

The image below shows a variety of microscope systems. There are multiple magnification objectives depicted: low power 4x, medium power, 40x and high power 100x. The distance from the end of the objective to the sample, which is called the working distance, is going to be larger on a low power objective, less on a medium power and very fine, possibly a fraction of a millimeter for the high power system. What’s critical is the angle. The sample is illuminated and light is coming out of it. In the case of the shorter working distance, higher numerical aperture objectives, that light is coming at an increasingly higher angle. The Numerical Aperture (NA) of the objective equals the sine of the half-angle (theta divided by two where theta is the entire angle). Read more on how to calculate the numerical aperture.

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Some of the better quality cameras had a turret with a capacity for two or more lenses for making rapid changes in focal length. Though zoom lenses were available in the 1940's, they were very expensive and involved some optical compromises, so were not widely used until they had achieved better and more consistent quality at lower prices. After this was achieved, they eventually came to replace the turret in the 1960's, particularly in cameras for the amateur film maker. The C-mount zoom lenses became dominating when some professional and amateur video cameras with C-mount appeared.

When designing automated digital microscopy devices for life science, biomedical, and diagnostic applications, typically, our goal is to optimize the device for the highest resolution image and the highest throughput in images per second. Microscope calculations such as magnification, resolution, microscope field of view, depth of field, and numerical aperture help us determine various aspects of a microscope’s capabilities and simplify the modeling and prototyping process. In this article, we will cover the importance of microscope calculations in optimizing microscope performance for your specific application.

The microscope field of view stands for the area of the sample visible through the microscope, which is calculated by dividing the sensor diagonal size by the magnification of the objective lens. For instance, a 20 mm diagonal sensor with a 20X objective lens would yield a Field Of View (FOV) of 1 mm, typical for microscopy applications. Microscope field of view can be calculated using the following formula:

Besides the magnification, reducing the size of the sensor down to the size of the field of view on the sample also does the same with the pixels. For example, a sensor with 4 micron pixels and a 40X objective would be 0.1 micron of geometric resolution or 100 nanometers. But it turns out it isn’t quite that simple. There’s a property of light that acts like a particle, the photon. It also has a wave property. The wave nature of light leads to a condition called diffraction, and due to diffraction, limits are set on resolution. To better understand how that works, we need to explore another concept, which is called numerical aperture.

Covers key formulas for selecting the optimal imaging sensor and microscope objective for your digital imaging application including sensor size, magnification, field of view, pixel sizes, resolution, depth of field, and numerical aperture.

Cheap C-mount adapters are available for the Nikon 1 cameras, but with those gadgets You get your MF lenses only utilisable in M mode of the camera. That means: in manual exposure setting mode. An alternative camera is the Samsung NX mini, but mechanical adapters aren't cheap, only made by one American maker. Of course these adapters enable convenient lens use in A mode, that means aperture priority with automatic exposure time setting, and that means all what C-mount adapters for µ4/3 mount deliver too. But they are not always available. For users of only small C-mount lenses the maker offers a CS mount adapter for the NX mini to which a light C-mount lens easily can be adapted, by means of a 5mm C-mount lens tube extension or a similar C/CS-mount adapter.

But the Nikon 1 system is not out of the game: A chipped adapter can simulate the presence of a system lens so far that automatic shutter speed setting will be usable, too. Of course this adapter isn't cheap, too, since only available from one Russian maker. Thus, both options, either a Nikon 1 camera or the Samsung NX mini, might work conveniently as C-mount-adapting camera, but both for an adapter price that, shipment included, might cost nearly as much as a used camera body.[1]

Microscope calculations are specific to the imaging sensor and microscope objective selection, which also impacts the performance requirements of the sample XY motion and Z focusing motion.

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The C-mount consists of a one-inch diameter cylinder threaded to a pitch of 1/32-in, or 32 threads per inch. It was designed specifically for lenses used on 16mm cine cameras. However, a small number of subminiature still cameras have been made using the same lenses, including the Schatz Sola, Optikotechna Mikronette and the Doryu pistol-shaped police cameras.

In this century, people have begun to experiment with C-mount lenses on digital (still) cameras, a trend boosted by the launch of the Micro Four Thirds (µ4/3) cameras which have an appropriate flange/sensor distance to allow adaption of the 16mm cine lenses.

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In this video, we will explain key optical imaging formulas and how they help in designing your automated digital microscopy imaging applications. Download Microscopy Calculator

For 20X magnification, which can be a numerical aperture of 0.6 to 0.8, the depth of field drops to about plus or minus 500 nanometers. Moving into high magnification with oil immersion at a numerical aperture of 1.47, the depth of field drops very dramatically, And the depth of field could be plus or minus 0.1 to 0.2 microns (100 – 200 nm). That’s a very tight tolerance. At 100 nanometers or 200 nanometers, very tiny changes in flatness of the sample or the height of the sample, or the precision of the guideways of the XY sample motion stage, will make it tricky to stay in focus. For maintaining focus in this situation, a continuous tracking laser auto-focus system connected to a high bandwidth objective focusing stage, such as the DOF-5, is the ideal way to maintain focus in these high numerical aperture, high-resolution applications.

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The same thing occurs in traditional photography, a very small aperture will increase the depth of field. A higher numerical aperture will give a higher resolution, but the depth of field becomes considerably smaller. There is a distance above the sample plane and a distance below the sample plane, and anywhere within them, there is essentially perfect focus. As soon as the objective and sample are outside of that boundary, the image begins to blur.

In most cases the material between the objective and sample is air, and “n” equals 1.00. For water, it’s refractive index is 1.33, and specialized immersion oil for microscopy is 1.52. For a low magnification objective such as a 4X or even a 10X, the typical depth of field is plus or minus 3 to 5 microns. In that case, if the sample is very flat, focusing may not be required at all, or only one needs to happen one time. Generally speaking, samples vary in thickness and they vary in flatness, so focusing is required.

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When video camera design evolved to modern camcorder design the interchangeable lenses vanished, but a few C-mount lenses are still produced for the market of surveillance cameras and industrial process control cameras.

C-Mount dimensions

To optimize the field of view for a particular application, it is vital to consider the size of the specimen and the level of detail required. For example, if a large sample with low detail is being observed, a lower magnification and larger field of view may be more appropriate. Conversely, if a smaller specimen with high detail is being observed, a higher magnification and smaller field of view may be necessary to achieve the desired level of resolution.

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The formats APS-C and even µ4/3 are not the ones which were and are in the focus of C-mount lens development. The maximum sensor size of survey cameras is smaller: 1 inch. That's more than 16mm film's 1/2" diagonal, so that several old 16mm movie lenses have a smaller image circle than 1". On the other hand there are survey lenses offered especially for 1" format, and some lenses for 2/3" format with longer focal lengthes may give a 1" image circle too. Thus system cameras with 1" sensors are more convenient concerning sufficient image circle or vignetting of adapted C-mount lenses.

Thread depths varied from maker to maker, but the proper A-B-C distinction lies not in the total thread depth but in the distance from the flange seat of the lens barrel to the innermost thread. The C-mount eventually became the standard mount for higher-quality amateur 16mm cine cameras.

The "C" designation derives from the evolution of the mount from its "A" and "B" predecessors. All shared the same thread and diameter, but had different mounting depths; the three are not interchangeable. A-mount and B-mount lenses were used primarily on Filmo cameras of Bell & Howell. The cine camera and lens maker began to feature interchangeable lenses earlier than its concurrents like Victor. The first C-mount camera was the Bell&Howell's Filmo 70 for 16mm film.

A larger field of view is generally desirable, as it allows for a larger area of the sample to be viewed at once, making it easier to locate and navigate to specific areas of interest. However, as the magnification increases, the field of view decreases, making it more difficult to observe larger areas of the specimen at higher magnifications. Need help with microscope calculations? Download our calculator.

where Sensor Diagonal is the diagonal size of the camera sensor in millimeters (similar to specifying a TV size), and Objective Magnification is the magnification of the objective lens being used.

To summarize, for high imaging resolution a high numerical aperture objective is required and one of the consequences of that is a fairly small depth of field which places an emphasis on the quality and performance of your focusing stage and XY sample positioning stage. Dover Motion has developed the DOF-5 specifically for microscope objective focusing.