mCherry proteinsequence

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Mcherry proteinfunction

The inspection site inside the object is not always positioned opposite the borescope or fiberscope. This is why there are different directions of view. This makes it possible, for example, to look to the side or diagonally to the front. The direction of view is specified in degrees in relation to the shaft. SCHÖLLY offers directions of view ranging from 0° - 110°.

The structure of mCherry protein consists of 3 alpha helices and the beta barrel that is made of 13 beta sheets. Its chromophore contains three amino acids, glycine, methionine and tyrosine. These amino acids are post-translationally modified into imidazoline. The red-shifted absorbance and emission of mCherry protein is due to its extended p-electron conjugation.

Flexible and rigid borescopes have a light guide connection to illuminate the inspection site via an external light source. The light guide connects the light source to the borescope or fiberscope. The light guide connector used in our borescopes/fiberscopes is a SCHÖLLY standard connector.

mCherryexcitation emission

In fiberscopes (also called flexible borescopes) the transmission of images and light takes place via image bundles. Image bundles consist of individual fibers which have the same relative position to each other at the input and output. Each fiber transmits a pixel from the objective to the ocular. The quality of the image depends on the number of pixels and the size of each individual fiber. The image bundle systems ensure the flexibility and movability of the probe.

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Mcherry proteinuses

The working diameter is the outer diameter of the borescope/fiberscope shaft. In principle, the working diameter selected should be as large as possible. However, the depth of field range and direction of view must also be taken into account. The working length is the length of the borescope shaft.

Martin Puebla, 2023, California State University, The Characterization of Methionine-Enriched Phytohemagglutinin-E Protein in Phaseolus vulgaris

mCherryvs GFP

Borescopes and fiberscopes are technical devices with which the inside of technical hollow bodies is viewed in the industrial sector. On this page you will find useful terms and explanations around the construction of endoscopes.

The field of view, also called angle of view or aperture angle, indicates the visible image section. It is specified in degrees. As of 80° and above, it is called a wide angle. The field of view is independent of the direction of view of the borescope/fiberscope. In its standard product range, SCHÖLLY offers fields of view ranging from 30° - 100°.

With a deflectable tip, flexible borescopes can be used to examine hollow spaces from various angles of view. The tip can be deflected in two-way or four-way direction via an adjusting lever on the device.

The ocular is the part of the borescope and fiberscope through which you look with the eye at the inspection site. For a digital display of the inspection, a camera/camera head can be connected to the ocular and the images can be displayed on a monitor. Our borescopes and fiberscopes are equipped with a DIN ocular and these fit all SCHÖLLY camera heads. With a SCHÖLLY endocoupler, borescopes can also be connected to other endoscopic cameras.

In general, a short focal length—or short focus, or wide-angle—lens is one whose angle of view is 65° or greater. Recall from above that angle of view is ...

mCherrymolecular weight

Mcherry proteinstructure

Obtained by dividing the focal length of the lens by its collimation clear aperture. Click link for detailed specifications on the substrate. Precision Aspheric ...

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Our products are distinguished by high quality and an ergonomic and user-friendly design. We base our product development on your needs that arise in everyday inspection tasks.

mCherry proteinsize

MCherry protein can be used as an intracellular probe in fluorescence microscopy. However, possible interactions between the tagged protein and the fluorescent protein can disturb the targeting process or the function of the protein of interest. mCherry protein can be used as an acceptor for hetero-FRET (also known as fluorescence resonance energy transfer) and probe for homo-FRET experiments. FRET is a nonradiative phenomenon that consists of energy being transferred from the donor (fluorophore) to the acceptor (chromophore). The efficiency of this lab technique depends of the distance between the donor and the acceptor. MCherry protein was developed in order to study bacterial gene expression in L.pneumophila, a Gram-negative pathogen responsible for the Legionnaire’s Disease. Because mCherry protein doesn’t interfere with other plasmids and doesn’t alter the intracellular, this protein has been frequently used for constitutive gene expression in a wide range of Gram-negative bacterial species.

Mirror tubes are attachments that the user can use to change the direction of view of the borescope. By turning the reflector tube during the inspection, the user can gain a 360-degree view. Mirror tubes are available with different directions of view. Directions of view of 70°, 90° or 110° are available.

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Plan Phase IOS objectives are a type of microscope objective lens that combines the benefits of plan and phase contrast microscopy. Like Plan IOS objectives, ...

They can usually pass long wavelengths only (longpass), short wavelengths only (shortpass), or a band of wavelengths, blocking both longer and shorter ...

For its universal borescopes, SCHÖLLY offers a range of interchangeable objectives and rotatable objective tubes with different directions and fields of view. The rotatable objective tubes can be used to gain a 360-degree view inside the inspection object. The user only needs a basic device to use the interchangeable objectives and rotatable objective tubes to adapt their equipment to different requirements within a similar diameter range. Directions of view ranging from 0° - 90° are available.

MCherry protein is a member of mFruits family of proteins which consists of monomeric red fluorescent proteins. These proteins are often used as tags to exploit different organelles using fluorescence spectroscopy. MCherry protein is capable of absorbing light between 540-50 nm and emits light in return in a wavelength range of 550-650 nm. MCherry protein has been used to visualize genes and further analyze their function. One main advantage of using fluorescent proteins such as mCherry and mRFPs, is that, because of their lower molecular weight, that have the tendency to fold fasters than tetramers. This results in monomeric fluorescent proteins that disturb the target less.

In order to detect potential areas of damage early on, SCHÖLLY develops products and solutions that provide the user with clear images of the inside of complex components and equipment.

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