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The resolving power of the objective is determined by the numerical aperture (not magnification). Numerical aperture also determines the light collecting ability of the objective, the higher the NA the more light the objective can collect and is determined by the function (nSinq, Where n =  immersion media refractive index, and Sinq = angle of the cone of illumination).

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Lens Tints Looking for a little color in your lenses? Maybe you want some computer glasses to reduce some of the blue light? Ask your optician about the full range of lens tint options that would enhance your eyeglass lenses for your current lifestyle!

Most microscope objectives are designed to be used with a cover glass that has a standard thickness of 0.17 millimeters and a refractive index of 1.515, which is satisfactory when the objective numerical aperture is 0.4 or less. However, when using high numerical aperture dry objectives (numerical aperture of 0.8 or greater), cover glass thickness variations of only a few micrometers result in dramatic image degradation due to aberration, which grows worse with increasing cover glass thickness. To compensate for this error, the more highly corrected objectives are equipped with a correction collar to allow adjustment of the central lens group position to coincide with fluctuations in cover glass thickness.

In order to allow the microscopist to quickly identify phase contrast objectives, many manufacturers inscribe important specifications, such as the magnification, numerical aperture, tube length correction, etc., on the outer barrel in green letters. This serves to differentiate phase contrast objectives from ordinary brightfield, polarized, DIC, and fluorescence objectives which either use an alternative color code or the standard black lettering.

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This excel spreadsheet has two worksheets that let you compare different objectives to determine their relative brightness

Not all objectives are made equal! Objectives for microscopes contain lots of very small and delicate lens to both magnify the image, as well as to perform a number of corrections (spherical and chromatic). Two main characterizations of objectives are the magnification and the numerical aperture.

Some objectives are also "Plan-" objectives, these are flat field corrected, so the image appears flat to both the eyepieces and the detector.

The microscope's resolving power depends on numerical aperture of the lens ... Earlier on, we said that the microscope is able to magnify and resolve an image, to ...

Correction collars are generally used to correct for spherical aberration due to variations in coverslip thickness, temperature, wavelength or for the differing refractive indices of different immersion media.

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After you've chosen your lens type, there are added features you may want to include as you order your perfect pair of eyeglasses or sunglasses. These features could be different types of coatings available, each which offers a special type of protection for your new lenses and for your eyes.

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There are different 'grades' of objectives which describe how many colours they are axially corrected for, and how many colours they are spherically corrected for.

Anti-Reflective Coating Anti-reflective coating or AR coating can make a huge difference in how comfortable you are looking through your new lenses. The special formulation of an AR coating is applied to both sides of the lens in order to dramatically lessen the quantity of glare that bounces off your lenses and into your eyes. A good AR coating helps block glare from sunlight, overhead lights, light from the computer display screen or even car headlights at night. If you hate the sensation of seeing your own eye look back at you because of glare in your glasses, an anti-reflective coating will eliminate that. An anti-reflective coating can make your glasses have a better appearance to other people as well. Because the glare is diminished, others who are looking at you are able to see your eyes directly without having the surrounding lights casting unflattering glares on the eyeglass lenses. An AR coating can be applied to sunglasses as well to help with the impact of sunlight as it passes through the glasses. This may enable you to see better with your sunglasses on a sunny day even if you have a lighter tinted lens. For those who choose AR coating, you'll want to ask about care of your lenses. Eyeglasses with AR coating will need to be cleaned with unique cleansers and should under no circumstances be cleaned with a dry fabric without wetting the lens first. This can result in scratches that will be very noticeable with an AR coated eyeglass lens.

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In theory, the intensity of illumination depends on the square of the condenser numerical aperture and the square of the demagnification of the light source image (in effect, the field diaphragm image becomes brighter as it is made smaller, according to the square law). The result is that brightness of the specimen image is directly proportional to the square of the objective numerical aperture as it reaches the eyepiece (or camera system), and also inversely proportional to the objective magnification. Therefore, when examining specimens in transmitted light, changing the objective without altering the condenser affects image brightness in response to changes in numerical aperture and magnification.

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Where F(trans) refers to image brightness of a Brightfield illuminated sample, whereas F(epi) refers to the image brightness of a fluorescence or reflected light illuminated sample.

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The terms F(trans) and F(epi) refer to the light-gathering power of an objective and were calculated according to the following equations:

A special objective is required that is fitted with a darkened circular ring or groove (phase plate) fitted into the glass near the rear focal plane of the objective as illustrated in Figure 1. In addition, the condenser must also be modified with special annular openings suited to a particular magnification and objective. Phase contrast objectives are segregated into a number of categories depending upon the construction and neutral density of internal phase rings:

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Collimators are required to transform naturally diverging light-emission from an optical fiber to a parallel beam of light. Most fiber-optic collimators ...

by K CONRAD · Cited by 16 — dx. )2 . The integral we want to calculate is A(∞) = J2 and then take a square root. Differentiating A(t) with respect to t and using the Fundamental ...

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Scratch-Resistant Coating Sadly, no eyeglass lens can be 100% scratch proof. A scratch-resistant coating is the next best thing! When you order this coating on your lenses, your optician will treat both the front and the back of the lenses with a special solution that will make them more durable and scratch-resistant. If you are the type of person who forgets your glasses case or you tend to clean your glasses with the bottom of your shirt, this type of coating will offer you some protection from shallow scratches. This lens coating is also great for children's eyeglasses.

NiSi Neutral density filters are made from high-quality optical glass with nano coating to prevent losing any single bit of detail. NiSi Neutral density filters ...

Image brightness is directly proportional to the objective NA and inversely proportional to magnification.  Therefore if you had two objectives of the same NA but differed in magnification, for examples the following;