Microscope Definition & Meaning - microscope description
They both are wonderful lenses you can’t go wrong. The 50mm is a cheap option for sharp portraits. The 35mm is a classic wider frame that allows you to still get crisp photos but gives you a little more room to work with. Lucky for you, I have a whole post that compares the two so you can decide for yourself which one is the perfect match for you. Check my post on 35mm vs 50mm.
A full frame camera is exactly what is sounds like, it will give you the full mm listed on your lens. If you have a 50mm lens, the focal length is 50mm.
Lenssharpnesscomparison
While there are some differences in the name brand lens compared to the third party lens, I don’t think it’s enough to make that much of a difference.
This is a beautiful lens for travel it has a wider aperture so it’s perfect for Group photography, landscape or street photography.
If you aren’t sure if you have a crop sensor or a full frame, you can do a quick search and find out since it will really help you determine which lens to buy.
The Digital pictureLens Comparison
This is going to be your go-to lens for environmental shots. It is a wide angle lens that allows you to get more in the frame. Because of that it might cause a little distortion.
There are so many different lenses out there that it can be overwhelming. All of the numbers and brands start to blur together. Let’s have a look at some of my favorites, and do a lens comparisons to make it easier for you when you’re ready for your next lens.
Canonlens comparisonchart
For a more detailed discussion of numerical aperture and resolution, CLICK HERE. Tube Length and Coverslip Thickness. The marks on the line below the the magnification and the numerical aperture are the tube length/coverslip thickness. The mechanical tube length (between the objective flange and the eyepiece seating face) is normally 160 (in mm) for older objective lenses or ( infinity for infinity-corrected objectives). The number after the slash is the thickness in millimeters of the cover glass for which the objective was designed and corrected. For most objectives for close working distance, this number is 0.17. This designation means that you should use No. 1½ coverglasses which range between 0.16 and 0.19 mm in thickness. No. 0, 1, and 2 coverglasses are not recommended. Some lenses will have a - sign. This means that the objective is meant to be used with no coverglass. LD (long working distance) objectives may go up to 1.5 mm so that one may look through slides or tissue culture flasks or dishes. Some lenses will also have a rotatable ring which allows one to correct for a coverslip thickness. They are sometimes labelled with "Korr." Objective Lenses Available This is a list of the objective lenses which are available for your use on the Zeiss LSM 310. They are all infinity corrected: Plan-Neofluars 10X/0.3 20X/0.5 40X/0.75 63X/1.25 Oil Iris 100X/1.3 Oil Back to Homepage
Nikonlens comparison
Read more about the gear I love:5 Reasons to Shoot with a Wide Angle LensCamera Lens Series: Everything you Need to KnowCanon Lens Comparisons
Canonlens comparisonphotos
Who doesn’t love new lenses? The way it changes how you shoot, the depth of field, and the beautiful aperture. I want to give you a super handy lens comparisons so you too can experience the new lens feeling.
For a more detailed discussion of numerical aperture and resolution, CLICK HERE. Tube Length and Coverslip Thickness. The marks on the line below the the magnification and the numerical aperture are the tube length/coverslip thickness. The mechanical tube length (between the objective flange and the eyepiece seating face) is normally 160 (in mm) for older objective lenses or ( infinity for infinity-corrected objectives). The number after the slash is the thickness in millimeters of the cover glass for which the objective was designed and corrected. For most objectives for close working distance, this number is 0.17. This designation means that you should use No. 1½ coverglasses which range between 0.16 and 0.19 mm in thickness. No. 0, 1, and 2 coverglasses are not recommended. Some lenses will have a - sign. This means that the objective is meant to be used with no coverglass. LD (long working distance) objectives may go up to 1.5 mm so that one may look through slides or tissue culture flasks or dishes. Some lenses will also have a rotatable ring which allows one to correct for a coverslip thickness. They are sometimes labelled with "Korr." Objective Lenses Available This is a list of the objective lenses which are available for your use on the Zeiss LSM 310. They are all infinity corrected: Plan-Neofluars 10X/0.3 20X/0.5 40X/0.75 63X/1.25 Oil Iris 100X/1.3 Oil Back to Homepage
This is my go-to sports lens. If you are wanting to shoot some action shots of your favorite football player, this is the lens you want.
I say ditch the kit lens. You can save your money and spend it on a better quality lens at the same price or maybe even cheaper. Go for a lens like the 50mm 1.8. I promise you it will be significantly better than the kit lens.
The higher the mm number on the lens, the closer it will be. So it will not always be better to have less space. Ultimately, only you can answer this question because it depends greatly on your subject matter and your photography style.
Lens comparisonchart
The “Nifty Fifty” is a great first lens to have! The lens boast a wide aperture, and it is small and easy to keep on your camera.
For example if you are in a house, you probably do not want to be using a 70mm-200mm zoom lens because you will not have much room in your frame.
Maybe you’re on vacation and you want some photos of the hotel you stayed in AND you want some close ups of the family. This universal lens will do it all.
Decide what your photography style is and go from there. Some people NEED a zoom lens and others need a fast smaller prime lens. Just try to keep the aperture wide since it will give you that professional blurred background.
A third party lens is a lens that is not made by the camera brand. That means Tamron, Sigma, and Tokina are all third party lenses.
Lens comparisonmm
The 35mm lens is a universal lens that is easy to use indoors or for travel. It’s small and lightweight but allows you to fit a lot in the frame without too much distortion.
A prime lens is one that has a fixed focal length. Meaning if you want more or less in your frame, you have to move your feet. Fixed means the lens doesn’t move once it’s on your camera.
A Lensbaby is a lens that is for the creative photos. It’s on ball and socket mechanism and allows you to do special effects. This lens probably won’t be your every day lens, unless creative photography is your thing. It sure is fun.
Lens comparisonwebsite
This section covers this subject because knowledge of the markings on an objective will give you the information concerning its proper use and whether it is suitable for the microscopic task you have in mind. Lens Type. The first thing that most lenses have is some lettering such as Plan-Neofluar, Plan Fluotar, Planapochromat, Plan or Achroplan. These are all different types of objective which have many glass, fluorite, or quartz elements for light path corrections. The types of lenses listed here are based on the Zeiss objectives as the Facility microscope is a Zeiss LSM 310. However, the names listed here should allow you to determine the type of objective from any manufacturer. If not, you will have to contact the manufacturer to explain the name and markings. The term Plan stands for flat field. Lenses which are uncorrected for flatness of field will have the center of the field in focus and the outer edges out of focus (or vice versa depending on how you focus the lens). So Plan means the lens is corrected to allow the whole field to be in focus. Achroplans are best for transmitted light while Epiplans are designed for reflected light use. Some microscope manufacturers will list their flat field achromatic lenses as simply "Plan". Achromat lenses have good color correction for two wavelenths of light. They are budget priced lenses. Planachromats are achromats with correction for flatness of field as well as the aforementioned color correction. Plan-Neofluar or Plan-Fluotar lenses are semiapochromatic lenses. They have good color correction for at least three wavelengths and also have the all around flatness of field. They are excellent for polarization microscopic techniques such as differential interference. As they also transmit UV very well, they are excellent lenses for all types of fluorescence microscopy. Any lens with the term fluar in it has fluorite elements in it and all of these are very good for fluorescence work. Zeiss recently introduced a new line of semiapochromatic lenses named Fluar lenses. These are objectives without a flat field made especially to increase the brightness of fluorescence. The image from a fluar lens is approximately 10% brighter than the equivalent Plan-Neofluar. In the UV range, the light transmission increases by 25-50%. This line of objective lenses was introduced about two years ago. Apochromatic lenses (Planapochromat)are the most highly color corrected objectives: they are corrected for four wavelengths and are top of the line in objective lenses. These most often have the highest numerical apertures (see below). Be careful in using these lenses for fluorescence, however. They do not transmit UV light. They work very well for visible light excitation in the blue and green ranges. Immersion. Lenses will be marked for the immersion medium in which they are designed to be used: (Oel) or (Oil) for oil. (W) for water immersion. (Imm) Multi-immersion, for oil, water, and glycerin. Phase marking. If the lens has a phase ring and can be used for darkphase illumination, the lens will be marked above the lens type with a "Ph" followed by a number corresponding to the manufacturer's phase ring number system for matching to a ring in the condenser. Phase lenses are generally not as good for fluorescence applications as the light transmission is reduced by the presence phase ring inside the lens. Magnification. As stated before, this is obvious and self-explanatory. Numerical Aperture. After the imprint of the magnification on any quality objective lens, there is usually a slash followed by a number which may be anything from 0.035 to 1.4. This number is the numerical aperture (N.A.) of the lens. This number is directly related to the resolution and second, for those of you doing fluorescence microscopy, it is related to the amount of brightness of the specimen brought into the lens (obviously very important for fluorescence microscopy!) The higher the N.A. of a lens the better its resolving power and the brighter the image it can produce. Resolution is defined as the ability of a lens to distinguish between small objects. Obviously, this differs greatly from magnification which is just the ability of the lens to enlarge the image of an object. It does not mean that you will necessarily be able to resolve details in the object. For a more detailed discussion of numerical aperture and resolution, CLICK HERE. Tube Length and Coverslip Thickness. The marks on the line below the the magnification and the numerical aperture are the tube length/coverslip thickness. The mechanical tube length (between the objective flange and the eyepiece seating face) is normally 160 (in mm) for older objective lenses or ( infinity for infinity-corrected objectives). The number after the slash is the thickness in millimeters of the cover glass for which the objective was designed and corrected. For most objectives for close working distance, this number is 0.17. This designation means that you should use No. 1½ coverglasses which range between 0.16 and 0.19 mm in thickness. No. 0, 1, and 2 coverglasses are not recommended. Some lenses will have a - sign. This means that the objective is meant to be used with no coverglass. LD (long working distance) objectives may go up to 1.5 mm so that one may look through slides or tissue culture flasks or dishes. Some lenses will also have a rotatable ring which allows one to correct for a coverslip thickness. They are sometimes labelled with "Korr." Objective Lenses Available This is a list of the objective lenses which are available for your use on the Zeiss LSM 310. They are all infinity corrected: Plan-Neofluars 10X/0.3 20X/0.5 40X/0.75 63X/1.25 Oil Iris 100X/1.3 Oil Back to Homepage
One reason I love the 35mm is because you can buy this lens with a wide aperture such as f/ 1.4. This gives you that beautiful blurred background without compromising your space.
I regularly use both a Tamron and a Sigma and I love them. I can honestly say that I can’t tell a difference in the quality.
As a beginner you will probably want a lens that allows you to have a wide aperture like 1.8. I love my 35mm and my 50mm. They are both prime lenses that are a cost efficient way to practice photography. You will also be able to use them as a professional. If you choose a zoom lens make sure to get a fixed aperture. Read more about photography gear for beginners.
Whereas the crop sensor has a crop of 1.6x (Canon) or 1.5x (Nikon). So on a crop sensor a 50mm lens will actually have the focal length of 80mm (Canon) or 75mm (Nikon).