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Hopefully, this overview has taken some of the mystery out of using your lab’s ancient light microscope. It can be a nightmare if you’re faced with using a microscope for the first time, but following these few, easy steps should ease the pain a little!

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Why do I see a cloud? When you are looking at protein localization within a cell, have you ever wondered why you see a cloud of fluorescence rather that several individual fluorescent points? Well, light microscopy has a theoretical resolution limit of 200 nm. This means that in theory, to resolve two points as being…

Dr Nicola Parry graduated from veterinary school at the University of Liverpool and spent several years working in mixed general practice in the UK before moving to the USA to pursue Anatomical Pathology at the American College of Veterinary Pathologists.

For a long time we’ve been able to pinpoint the subcellular location of proteins, and the advent of FISH (Fluorescence in situ Hybridization) allowed us to locate the position of genes in the nucleus, but recent advances in RNA FISH are making it easier and easier to collect the same data about individual messenger RNAs….

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If you’re starting your PhD or post-doctoral work, chances are you’ll need to use a light microscope at some stage during your research. Some of you may be seasoned microscopists. For many of you though, this might be the first time you’ve ever plugged in a microscope, or at least the first time you’ve used one since some long-forgotten undergraduate histology course. Yet somehow you’re expected to just dust off that old ‘scope in the corner of the lab, and start using it as if you’re an old hand. Have no fear – help is at hand! In today’s article, I’m going to go over the basics of the light microscope.

Have you ever been shown how to use a microscope properly? Or do you just dive right onto the microscope with little or no training and scant knowledge of the basics, then twiddle knobs, snap photos and expect the publication-quality images to appear? If it’s the latter you are certainly not alone! If only there…

Like most things in this world, fluorophores are mortal, and eventually your once bright fluorescent image will inevitably fade to black. This fading or ‘photobleaching’ of fluorescent signal can make imaging difficult, especially if you are trying to take quantitative images. Read below to learn what causes photobleaching of your fluorophores and how best to…

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To begin with, I’d like to introduce you to the parts of the microscope – basically the bits that might be useful for you to know about. No optical physics required!

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Ever wake up especially groggy in the morning, finding it takes a few minutes and a few eye rubs to be able to decipher the numbers on your alarm clock? Our eyes have the ability to resolve an image, so that you can observe separate objects and details. Similarly, microscopes have a parameter of resolution:…

Read More Create Publication Quality Fluorescence Microscopy Images with the Help of Leica Microsystems’ Science Lab ResourceContinue