Olympus25x 1.05 NA

There are several other objective lens magnifications available with utility for particular applications. The 2x objective, widely used in pathology, has only ½ the magnification of a 4x scanning lens, thus providing a better overview of the sample on the slide. The 50x oil immersion objective, often used in place of the 40x objective, is used as a gold standard for observing blood smears. The 60x objective, often available in either dry or oil immersion, provides 50% greater magnification than a 40x lens. The 60x dry is sometimes chosen over a 100x oil immersion lens for higher magnification without the need to use immersion oil.  Finally the 100x dry objective doesn’t need immersion oil to deliver high magnification (still 1000x when combined with 10x eyepieces). However, the numerical aperture (an indication of resolving power of an objective) of a 100x dry objective is much lower than that of a 100x oil immersion objective and, as a result, the ability of the lens to resolve fine details in the specimen is much lower, too.

To calculate the FOV requires the sensor size and the focal length of the lens: h = Sensor Size F = Focal Length of the Lens

It is important to always use the correct immersion media (e.g. air, water, oil, etc.) that is specified by your objective lens.

Olympuslife science

A narrow angle lens or larger lenses produce a smaller field of view; capturing a limited area, but the camera quality image improves in detail. These are designed to monitor a specific object, such as cash registers, entrances/exits, hallways or objects of value.

Uplansapo

You can find the camera’s Field of View details in the Specifications sheet. Click here for additional support content including security camera documentation.

The low power objective lens has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples. The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.

A wide angle lens or smaller lenses produce a greater field of view and captures more objects in a scene enhancing your ability to cover larger and wider areas such as foyers, parking lots or warehouses.

The oil immersion objective lens provides the most powerful magnification, with a whopping magnification total of 1000x when combined with a 10x eyepiece. But the refractive index of air and your glass slide are slightly different, so a special immersion oil must be used to help bridge the gap. Without adding a drop of immersion oil, the oil immersion objective lens will not function correctly, the specimen will appear blurry, and you will not achieve an ideal magnification or resolution. Oil immersion lenses are also available from some manufacturers in lower magnifications, and provide higher resolution than their "high dry" counterparts.

Zeissobjective

Field of View (FOV) is the maximum observable area that is seen at any given moment through an optical device such as a camera lens. The coverage of the area can be measured using the horizontal and vertical distances to find the diagonal of the area in degrees. Mathematically, the FOV is calculated using the horizontal dimension of the sensor (h) and the Focal Length (F).

If you are interested in buying various types of objective lenses for your microscope in the classroom, laboratory, research facility, or any other purpose, ACCU-SCOPE can provide the products you are looking for. Contact us today to learn more about our objective lenses and other microscope accessories.

MitutoyoObjective lens

Field of View (FOV) is the maximum observable area that is seen at any given moment through an optical device such as a camera lens. The coverage of the area can be measured using the horizontal and vertical distances to find the diagonal of the area in degrees. Mathematically, the FOV is calculated using the horizontal dimension of the sensor (h) and the Focal Length (F). You can find the camera’s Field of View details in the Specifications sheet. Click here for additional support content including security camera documentation. The Camera Lens, Sensor and Focal Length Focal length (F) is the distance between the center of a lens and its sensor. The size of the lens is the aperture size. These factors affect field of view. A narrower focal length captures more of the scene and displays a larger field of view. A wider focal length magnifies a scene and decreases the field of view. The higher the focal length value the lower the FOV. Types of Camera Lenses Fixed: a fixed camera focal length provides an Angular FOV which is permanently set and cannot be adjusted by the user. Varifocal: the camera focal length can be manually adjusted by the user. Generally, this is done manually with screws or dials. At Lorex we have motorized varifocal cameras that allow you to digitally zoom using your phone or NVR without losing details. These lenses provide flexibility and customization for your camera image needs. The importance of Field of View A wide angle lens or smaller lenses produce a greater field of view and captures more objects in a scene enhancing your ability to cover larger and wider areas such as foyers, parking lots or warehouses. A narrow angle lens or larger lenses produce a smaller field of view; capturing a limited area, but the camera quality image improves in detail. These are designed to monitor a specific object, such as cash registers, entrances/exits, hallways or objects of value. How to Calculate the FOV To calculate the FOV requires the sensor size and the focal length of the lens: h = Sensor Size F = Focal Length of the Lens FOV is represented by this equation: FOV = 2tan-1(h) / 2F Example: h = 4.7mm F = 6mm FOV = 2tan-1(h) / 2F = 2tan-1(4.7)(12) = 2tan-1(0.39) = 2(21.4°) = 42.8°

Olympusuis2

Fixed: a fixed camera focal length provides an Angular FOV which is permanently set and cannot be adjusted by the user. Varifocal: the camera focal length can be manually adjusted by the user. Generally, this is done manually with screws or dials. At Lorex we have motorized varifocal cameras that allow you to digitally zoom using your phone or NVR without losing details. These lenses provide flexibility and customization for your camera image needs.

OlympusVS200 software

A scanning objective lens provides the lowest magnification power of all objective lenses. 4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of 40x. The name “scanning” objective lens comes from the fact that they provide observers with about enough magnification for a good overview of the slide, essentially a “scan” of the slide. Some objectives with even lower power are discussed in Specialty Objectives below.

JavaScript seems to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website.

Focal length (F) is the distance between the center of a lens and its sensor. The size of the lens is the aperture size.

OlympusUPLFLN

The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample. The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.

These factors affect field of view. A narrower focal length captures more of the scene and displays a larger field of view. A wider focal length magnifies a scene and decreases the field of view. The higher the focal length value the lower the FOV.

Most compound microscopes come with interchangeable lenses known as objective lenses. Objective lenses come in various magnification powers, with the most common being 4x, 10x, 40x, and 100x, also known as scanning, low power, high power, and (typically) oil immersion objectives, respectively. Let’s take a closer look at each of the different magnifications of objective lenses and when you would use them.