Ans. A microscope is an optical instrument with one or more lens systems that are used to get a clear, magnified image of minute objects or structures that can’t be viewed by the naked eye.

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Microscope parts and functions

RECOMMENDED OBJECTIVES IN SITU HYBRIDIZATION · Plan objectives optimized for fluorescence microscopy; High numeric aperture (~1.3) · Objectives with the fewest ...

The optical parts of the microscope are used to view, magnify, and produce an image from a specimen placed on a slide. These parts include:

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1. Illuminator (Light Source)2. Diaphragm (Iris)3. Condenser4. Condenser Focus Knob5. Rack Stop6. Stage7. Stage Control Knobs8. Nose Piece9. Objective Lens10. Tube (Head)11. Eyepiece (Ocular Lens)12. Diopter Adjustment13. Adjustment Knobs (Fine Adjustment Knob and Coarse Adjustment Knob)14. Arm15. Base16. Light Switch17. Brightness Adjustment

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Ans. The eyepiece, also known as the ocular is the part used to look through the microscope. Its found at the top of the microscope. Its standard magnification is 10x with an optional eyepiece having magnifications from 5X – 30X. Objective Lens are the major lenses used for specimen visualization. They have a magnification power of 40x-100x. There are about 1- 4 objective lenses placed on one microscope, in that some are rare facing and others face forward.

A beam of light is passed through the condenser to the specimen. The light transmitted from the specimen enters the objective lens. While passing through the objectives, the transmitted rays are spread so that they appear to come from the bigger objects.

The technical engineers behind the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM super-telephoto lenses were tasked with enabling photographers to get closer than ever before to hard-to-reach subjects, whether that be birds and wildlife, athletes on the track or field, or disaster scenes and war zones. Taken on a Canon EOS R5 with a Canon RF 1200mm F8L IS USM lens and a Canon Extender RF 2x at 2400mm, 1/500 sec, f/16 and ISO8000.

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Canon is synonymous with super-telephoto lens innovation. From FD through to EF and the latest RF primes and zooms, Canon's groundbreaking long lenses have been relied on by professional sports and wildlife photographers for decades. With the launch of the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM, another milestone in mirrorless camera lens design has been achieved. No other lenses in their class come close to offering the combination of extreme reach, compact size, fast AF and outstanding optical quality.

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Microscopes are made up of lenses for magnification, each with its own magnification powers. Depending on the type of lens, it will magnify the specimen according to its focal strength.

The design priorities for the Canon RF 1200mm F8L IS USM were to achieve both a longer focal length and a significantly smaller size and lighter weight, explains Tomohiro Ino, "while maintaining the high image quality that is expected of the latest super-telephoto L-series lenses". Taken on a Canon EOS R3 with a Canon RF 1200mm F8L IS USM lens at 1200mm, 1/1250 sec, f/8 and ISO500.

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"Since we are always working on technological innovation, the technology adopted for these lenses may naturally be used for future RF lenses," says Katsuhiro Inoue. "We will continue to pursue more innovative technologies and develop attractive products that will surprise our customers. Please stay tuned." Taken on a Canon EOS R5 with a Canon RF 800mm F5.6L IS USM lens and Canon Extender RF 2x at 1600mm, 1/800 sec, f/11 and ISO1250.

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"It's important to have a lens hood that keeps cool air in front of the optics, as that can also potentially improve the optical quality," adds Mike. Taken on a Canon EOS R3 with a Canon RF 800mm F5.6L IS USM, 1/2000 sec, f/5.6 and ISO3200.

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Pairing the Canon RF 1200mm F8L IS USM with the Canon Extender RF 2x delivers unparalleled optical quality, even at 2400mm. Taken on a Canon EOS R5 with a Canon RF 1200mm F8L IS USM lens and a Canon Extender RF 2x at 2400mm, 1/000 sec, f/16 and ISO6400.

The development of the pioneering EOS R System presented Canon's lens engineers with the opportunity to go back to the drawing board with these extreme focal lengths. Freed from the constraints of conventional lens design, it has been possible to reevaluate how a super-telephoto lens is made, resulting in the technical innovation and cutting-edge performance of the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM lenses. So, what were the challenges of constructing these super-compact, state-of-the-art super-telephoto lenses, and how have they been overcome? Here, the Canon lens development team takes us behind the scenes and provides insights into the design, engineering and operation of the Canon RF 800mm F5.6L IS USM and the world's longest focal length AF lens for mirrorless cameras, the RF 1200mm F8L IS USM.

Ans. The magnification of a lens is defined as the ratio of the height of an image to the height of an object. Microscope magnification measures the total enlargement of the image of an object. Magnification power is the product of eyepiece lens power and objective lens power.

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Functionof body tube in microscope

Microscopes are generally made up of structural parts for holding and supporting the microscope and its components and the optical parts that are used for magnification and viewing of the specimen images. Modern microscopes have additional electronics and display devices. This description defines the parts of a microscope and the functions they perform to enable the visualization of specimens.

There are different types of microscopes like light microscope, dark-field microscope, phase contrast microscope, electron microscope, fluorescent microscope, etc.

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Ans. The coarse adjustment knob moves the stage up and down to bring the specimen into focus. The fine adjustment knob brings the specimen into sharp focus under low power and is used for all focusing when using high-power lenses.

EyepieceTube

The light is then focused on the eyepiece lens. This lens further magnifies the pre-magnified image coming from the objectives.

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What iseyepiecein microscope

1. Ocular Lens (Eye Piece)2. Diopter Adjustment3. Head4. Nose Piece5. Objective Lens6. Arm (Carrying Handle)7. Mechanical Stage8. Stage Clip9. Aperture10. Diaphragm11. Condenser12. Coarse Adjustment13. Fine Adjustment14. Illuminator (Light Source)15. Stage Controls16. Base17. Brightness Adjustment18. Light Switch

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"Although the Canon RF 800mm F5.6L IS USM is longer than the RF 400mm F2.8L IS USM, and the RF 1200mm F8L IS USM is longer than the RF 600mm F4L IS USM, their overall length did not increase significantly due to the configuration of the new magnification optics," explains Mechanical Design specialist Takami Hirasawa (left), with Mechanical Design Leader, Katsuhiro Inoue.

Ans. Rack stop is included in the microscope for preventing the specimen slide from coming too far up and hitting the objective lens.

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The development team for the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM (left to right): Katsuhiro Inoue (Mechanical Design Leader), Takami Hirasawa (Mechanical Design), Hiromi In (Electrical Design) and Tomohiro Ino (Optical Design).

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Compared with the Canon EF 800mm f/5.6L IS USM, the RF 800mm F5.6L IS USM is around 3cm shorter and 1.36kg lighter, and the focusing distance has been halved. The size and weight savings that have been made with the Canon RF 1200mm F8L IS USM compared with the legendary EF 1200mm F5.6L USM are even more impressive. Not only is it around 30cm shorter and a staggering 13kg lighter, it focuses down to 4.3 metres – approximately 10 metres closer than the EF version. So how was this impressive feat achieved? The breakthrough came with the novel idea of positioning the magnifying optics near the back of each lens, explains the development team's Mechanical Design Leader, Katsuhiro Inoue. "The RF 1200mm F8L IS USM shares a similar optical design with the RF 600mm F4L IS USM but introduces a new dedicated magnifying optical system that has made it possible to significantly reduce the size of the lens compared with the EF 1200mm F5.6L USM."

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Find out how the tech in Canon's IS lenses works to keep images sharp despite camera shake, which IS mode to use for best results, and more.

Objectivelens function

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Ans. Condensers are lenses that are used to collect and focus light from the illuminator into the specimen. They are found under the stage next to the diaphragm of the microscope. They play a major role in ensuring clear sharp images are produced with a high magnification of 400X and above. Abbe condenser is a condenser specially designed for high-quality microscopes, which makes the condenser to be movable and allows very high magnification of above 400X. High-quality microscopes normally have a high numerical aperture than objective lenses.

The innovative optical arrangement of the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM means that the weight of the lenses is distributed more centrally compared with their front-heavy EF counterparts. Factor in powerful Image Stabilizers, and you've got a brace of super-telephotos that are practical to use handheld, as well as a highly mobile solution for tripod or monopod work. "We have a long history of pursuing compactness, reduced weight and high image quality in our super-telephoto lenses," says Katsuhiro Inoue. "In the process, for example, in terms of materials, we have adopted a magnesium alloy that has both high rigidity and light weight, which was not used in the era of the Canon EF 1200mm f/5.6L USM lens. As simulation technology has evolved, it has also become possible to reinforce, thicken/thin and optimise the structure of parts with dramatically higher accuracy than before." Both the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM are fully weather sealed and have specially strengthened barrels. "In terms of the mechanical design, the biggest challenge has been to ensure the parts that hold the magnification optics securely in place are strong enough," says Takami Hirasawa. "Our simulation technology has been very useful to help develop a lightweight and robust solution. Additionally, we have incorporated measures to hold the heavy lens elements more firmly while maintaining optical performance."

It is already possible to achieve focal lengths of 800mm or 1200mm in the RF L-series range, by attaching the Canon Extender RF 2x to the RF 400mm F2.8L IS USM or RF 600mm F4L IS USM. "However, by optimally designing the rear magnifying optics in the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM, the image quality is higher than when the extender is attached to the shorter lenses," says Tomohiro Ino. Not only do the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM give you better image quality, you also have the option to go even longer with the addition of the Canon Extender RF 1.4x or Extender RF 2x. "With focal lengths of up to 1600mm and 2400mm achievable, each combination brings a new means of professional photographic expression," adds Tomohiro.

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It's a similar story with the Canon RF 800mm F5.6L IS USM. The front half of the lens shares similar functionalities with the Canon RF 400mm F2.8L IS USM and it is compatible with the same lens hoods and accessories. But moving the new magnification optics closer to the camera enables the RF 800mm F5.6L IS USM to be much smaller and lighter than the Canon EF 800mm f/5.6L IS USM. "In the past, these big magnifying optics have been at the front of the lens, with the focusing group at the back," says Canon Europe's Senior Professional Imaging Product Specialist, Mike Burnhill, "but with the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM we've essentially flipped it around. Putting the magnification optics at the back, nearer the camera sensor, means that the optics no longer need to be, say, 160mm in diameter, which massively reduces the weight and size." To correct for chromatic aberration and ensure a high-quality image, a UD lens element has been incorporated into the design of the new magnification optics, explains Optical Designer Tomohiro Ino. "In addition to this, ASC coating that significantly suppresses flare and ghosting is used in those parts of the lenses that have been inherited from the RF 400mm F2.8L IS USM and RF 600mm F4L IS USM. We have also applied a fluorine coating to the front of the lens so that it is easy to remove any dirt and dust that adheres to the surface of the glass."

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The RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM lenses shared the same three key design priorities: "To achieve further focal lengths, higher image quality and improved mobility in order to meet the needs of today's super-telephoto lens users," says the development team's Mechanical Design Leader, Katsuhiro Inoue.

The Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM are markedly smaller and more lightweight than their EF counterparts. They also focus considerably closer, allowing approaching athletes, animals and other active subjects to be tracked over a greater distance than before.

The Canon RF 800mm F5.6L IS USM is smaller and lighter than the Canon EF 800mm f/5.6L IS USM (top). "The RF 800mm F5.6L IS USM is quite easy to use handheld, especially compared to the EF version," says Canon Europe's Senior Professional Imaging Product Specialist, Mike Burnhill. "Having the weight in the centre of the lens means that you get a lovely balance: you're able to support part of the weight with the camera body and part of the weight with your left hand, unlike a conventional front-heavy lens where the load is pretty much all on your left hand."

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"The UD lens located in the rear magnifying optical unit of the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM corrects the magnified chromatic aberration and achieves higher image quality than when using an extender with the RF 400mm F2.8L IS USM or RF 600mm F4L IS USM," says Optical Design specialist, Tomohiro Ino.

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As with the Canon RF 400mm F2.8L IS USM and RF 600mm F4L IS USM, the new lenses support electronic full-time manual focus. The ring-type USM motors used in the Canon RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM feature 'power-doubled focus drive', which allows two power inputs, enabling users of the EOS R3, with its high-capacity battery, to experience faster autofocusing. "The AF performance of these two lenses is in fact fast enough for general shooting without the need for the dual focus power drive," says Electrical Design specialist, Hiromi In. "However, the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM will benefit from this feature when the focus preset function is used." As well as being able to rapidly jump between two focus presets when shooting stills, this function can also be used to achieve smooth focus pulls when shooting video with the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM. "Both of these lenses also support ultra-low speed drive of the USM motor for movie shooting," says Hiromi. "In combination with the EOS R5, EOS R6 and later EOS R System cameras (including the EOS R5 C), the focus drive is much smoother."

Eyepiece lensmicroscope

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Their ability to function is because they have been constructed with special components that enable them to achieve high magnification levels. They can view very small specimens and distinguish their structural differences, for example, the view of animal and plant cells viewing microscopic bacterial cells.

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Its bright lighted area and magnification cause havoc with your eyes. And stitching with daylight lamps when it's not daylight can cause problems with sleep ...

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Having been constructed in the 16th Century, microscopes have revolutionized science with their ability to magnify small objects such as microbial cells, producing images with definitive structures that are identifiable and characterizable.

Objectivelensmicroscopefunction

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What is thefunctionof arm in microscope

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Microscopes are instruments that are used in science laboratories to visualize very minute objects, such as cells and microorganisms, giving a contrasting image that is magnified.

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The RF 1200mm F8L IS USM's maximum aperture is one stop smaller than that of the EF 1200mm f/5.6L USM, but this has considerable benefits for handling. "The f/8 maximum aperture contributes to the substantial reduction in size and weight compared to the conventional EF model," Tomohiro Ino confirms. "As the RF 1200mm F8L IS USM supports Dual Pixel CMOS AF, it is capable of fast and accurate autofocus despite the darker maximum f-stop. High image quality, autofocus and IS are also still achievable when the Extender RF 1.4x or RF 2x is attached to the RF 800mm F5.6L IS USM or RF 1200mm F8L IS USM." Indeed, the only discernible effects with the lens extenders attached are the drop in maximum aperture and a reduction in the AF coverage. "When the RF 800mm F5.6L IS USM is used with the Canon EOS R3, EOS R5 or any other camera that supports Dual Pixel CMOS II AF, you get full focus tracking across 100% of the frame, even with Extender RF 1.4x attached," explains Mike. "With the Extender RF 2x, you still get 80% AF coverage horizontally and vertically. "The RF 1200mm F8L IS USM still gives you 80% AF coverage horizontally and vertically with the Extender RF 1.4x, and it drops to 60% x 40% coverage with the Extender RF 2x. So, even with a 2400mm f/16 lens, you're getting a focusing area that's roughly the size that DSLR users are accustomed to. There's no impact on AF performance, apart from the size of the area."

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Heat-resistant paint is applied to both the lens barrels and lens hoods of the RF 800mm F5.6L IS USM and RF 1200mm F8L IS USM. "It's important for these types of lenses, because they're going to be shooting over greater distances and therefore potentially impacted by heat haze," says Mike. Taken on a Canon EOS R3 with a Canon RF 800mm F5.6L IS USM lens at 800mm, 1/2000 sec, f/5.6 and ISO800.

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