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Observing a leaf at 250x, we can see tiny round structures (I think barely bigger than what can be possibly seen by our eye): are those the cells?

25X is a mighty strong ocular (eyepiece) lens, certainly for home use. This means with the 40X objective you are already at 1000X overall. It would take a seriously thin, well mounted and well lit specimen for you to see a good image. This assumes the optics are of sufficient quality.

How tofocus a microscope step by step

Also, as others mentioned, using a 25x eyepiece in combination with a 40/0.65 or a 100/1.25 is kind of overkill. With that combination, you enter the realm of "empty magnification". In short: structures appear larger, but no new structures are revealed and the image becomes more and more fuzzy.

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And yes: you need to use immersion oil with the 100x objective, but keep in mind this is a very demanding objective to be used, on the part of the microscopist as well as on the part of the slide preparer.

I suppose you mean you put a leaf fragment between two regular microscope slides, those measuring 26mm * 76mm (1in. * 3in.)?

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At the 400X, or higher, magnification you will need to slice tissue very thin. But there are a couple ways to view cells without the knowledge or tools for preparing sections. To view plant cells, do an internet search for "onion cell wet mount". To view some animal cells (your son's), search "cheek cell slide".

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10 stepsinusing microscope

To focus the slide at 40X objective; at first focus it with 10X objective. Then rotate the turnstile at nose-piece and bring 10X objective. (In some lower quality microscope with the adjustment screws you may need to bring the main-portion of microscope upwards, distant from the slide. Otherwise the long 40x lens may hit the slide or drag the coverslip. However good-quality microscopes doesn't have this problem). Now it is time to focus the 40X.

To easily do that; at first bring the 40X lens tip just 1 or 2 mm distance from cover-slip. (Just like sometimes while a known-titration we blow the titrant from the burette quite high-rate upto certain value; and subsequently slow-down). Now take only the fine-adjustment screw and bring the objective backwards (and very carefully forwards... so that the lens doesn't hit the cover slip).

The 100X objective lense is usually a Oil-immersion lens. I.e. between the coverslip and 100X lens tip; the light pass through a specialized oil instead of air.

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Note-3: For common purpose we do not need the oil-immersion lens. It is used only to view very tiny things like chromosomes, bacteria, cell-wall ornamentation, pollen walls, fungal spores and such type of things. Average plant and animal cells' contours (and tissues) could be seen well under the 10X objective and 10X to 15X eyepiece. Sometimes students' microscope come without the 100X objective; the place for it on the turnstile kept closed with a screw-cap.

Note-1: Cleaning of oil immersion lens: Do not use xylene (though some protocol says that). It damages the eyepiece, cause blur, and may make it permanently non-functional. Most of our university-teachers forbidden strictly to use xylene for lens cleaning. Use a soft cotton cloth, at first moist, then dry (or alternatively wet and dry for several times). However cotton and xylene to be used to clean the coverslip quickly with minimal damage/friction of the microscopic specimen.

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I recommend using a 10X objective. This will give you 400X with the 40X objective. To use the 100X objective, you will need to insert a drop of oil between the objective and the slide. "Immersion oil" is made especially for this purpose.

Note-2: Use thin and uniform cover-slip (such as Blue-star (trade-name)) and try to make your sections more thin. See if that cause any improvement.

Using a microscope is not all that simple for novices, but there's an easy way to find out -if no image can be produced-, if it's a microscope problem or an inexperienced user problem: take as a specimen an as thin as possible large object, such as a leaf of cigarette paper, and try to focus on it, using low power and gradually use stronger objectives up to 40/0.65. If an image can be produced, even if it's rather blurred at 40/0.65, the microscope is okay.

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On the 100x objective lens "OIL" is written; does it mean the observation needs to be done through oil instead of air? Or just that oil is used inside the objective?

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Are you sure it can't be focussed? Objective lens like 40x or 45x normally have very near-focus (can be less than 1 mm) and also a shallow depth-of-field.

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In the... well... regular microtechnique, a specimen is put on a slide and covered with a very thin coverslip, having a thickness of around 0.15 - 0.17 mm.

That won't work. Long story short: microscope optics/objectives are designed with a specific working distance in mind, which is not an entiraly free choice, but dictated by the laws of physics/optics. An average achromat 40/0.65 has a working distance of about 0.6 mm. An average achromat 100/1.25 has a working distance of about 0.2 mm. An average microscope slide has a thickness of about 1 - 1.2 mm...

How to put 10x objective in place inmicroscope

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