What is depth of field microscopeformula

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Depth of fieldvsdepth offocusmicroscope

The depth of focus (DOF) or the focal depth is the range of distances for which the specimen (object) is imaged with an acceptable sharpness on the image plane. DOF is larger as the opening (aperture) angle of the incident probe is smaller but is smaller as the magnification of the image is higher. The opening angle of the incident electron probe of the SEM is about 100 times smaller than that of the optical microscope. Thus, the DOF of the SEM is about 100 times larger than that of the optical microscope. Fig. 1 schematically shows the change in DOF when the diameter of the objective lens aperture is changed. Fig. 2 shows a comparison of SEM images taken at the different DOFs determined by the different objective lens aperture sizes.

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Depth offocus in optics

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Depth offocus definition

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Depth offocus formula

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