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Obliquelight examination

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I’d also like to give a bit of a ‘shout out’ to a company – J.B Microscopes Ltd. When I bought the 40x Dplan Apo UV objective on eBay, the coverslip thickness correction collar was stuck and wouldn’t move (as far as I know the objective was bought new and then kept in a cupboard for 40 years, so presumably the grease had set). J.B Microscopes were able to fix this for a very good price and send it back to me within a few days of sending it to them, so I would definitely consider using them again in the future.

In response to the dire need on Guam, Core Tech redeveloped and repurposed an abandoned Naval housing facility into a multi-use educational campus that includes a high school, charter school and the new headquarters for the Guam Department of Education.

Core Tech is a major participant in the military buildup related to the base realignment of American military forces in the Pacific.

Oblique lightingForensics

We continue to look for opportunities to better our community and to expand on the economic growth our islands are realizing now and towards the future with the continuous growth of tourism and the impending military buildup.

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Oblique lightingexample

Our companies include one of the largest construction companies in Micronesia in Core Tech International. We are also proud to be the largest real estate development and property management firm in Micronesia with Core Tech Development.

Our business portfolio includes other companies such as a local daily newspaper in the Guam Daily Post, Core Tech Concrete, Core Tech Captive Insurance and Core Tech Renewable Energy.

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What isbouncelighting

Core Tech is a multi-industry conglomerate that is looking to broaden its business portfolio. We currently comprise of a diverse group of companies in finance, real estate, and construction as our core businesses.

So what is oblique illumination? Basically, it is offsetting the direction of the incoming light to the subject compared with normal bright field microscopy. Changing the angle of light emphasizes some features within a subject, and the direction of the incoming light can be altered to highlight specific features depending on their orientation. As an example let’s take a look at three images of a Synedra superba diatom, captured using an Olympus 40x Dplan Apo UV objective, using 405nm light, on my Olympus BHB. Firstly, normal bright field, with the light coming from directly below.

Frontlighting

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It is interesting to note in this image the ‘imperfections’ in the ‘perfect’ structure. However as mentioned, even this was reduced in resolution for sharing. Below we go in even tighter, and keeping the original image resolution.

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I’m a bit of an imaging geek, so at this stage I’ll show the objectives and condenser that were used for this work, and the slide itself (which cost me about £30 on eBay).

For financing, we are a US Treasury-certified Community Development Financing Institution boasting one of the largest investments and loan portfolios of any non-bank in Micronesia in Core Tech Capital.

Obliqueillumination microscopy

Using shorter wavelength UV light for microscopy can (and does) improve resolution, allowing smaller features to be resolved than with longer wavelength light. There are however times when using UV is impractical, or impossible, and in those circumstances, other approaches to improve the image are required. Today I’ll cover some basics about one of those approaches – the use of oblique lighting to illuminate the subject. With oblique lighting, we get to emphasize certain features on a subject, and this approach was used to capture the image shown below using my custom modified Olympus BHB microscope.

I hope that has been interesting for you to see. Microscopy continues to amaze me, as do the details I am able to see with my little custom made Olympus BHB UV microscope. Thanks for reading, and if you’d like to know more about this or any other aspect of my work, you can reach me here.

Transmittedlighting

Our experienced and flexible management team is noted for acquiring businesses and selecting management teams to propel the companies to higher levels of success. Core Tech’s most recent purchase of The Guam Daily Post, a local independent newspaper, continues to grow in revenue and distribution.

The oblique illumination gives an almost 3D appearance to the image which I really like, and there is certainly plenty of resolution given the NA of the objective (NA 0.85). Note, I have flipped this horizontally, as I like the composition better this way round. However I still wanted to go further, so I broke out the 60x Olympus SPlan Apo (NA 1.4) oil immersion objective. The objective and condenser were oiled to the slide and this is what an image from that looks like using 405nm oblique illumination.

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Core Tech has built hundreds of homes, low-income housing developments, senior housing divisions, commercial complexes, and hotel renovations. Of these, we are proud to showcase the newly-completed Summer Homes, Summer Green, and Summerville complexes as well as the ongoing Summer Oceanview Towers project, which will feature a mix of for-sale and rental units along with hotel, restaurant, and retail space.

As can be seen from the images, using oblique illumination highlights certain features, and by changing the direction the light comes in from different features can be emphasized.

Oblique lightingphotography

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Continuing with our diversification efforts, we are expanding into restaurants, cafes, farms and similar businesses to complement our real estate mix. We have also financed tried-and-true businesses such as Sejong Restaurant; a popular Korean cuisine establishment, and California Mart; a longstanding grocery store.

Our company is currently bidding on major upcoming military projects, and we have already been awarded, along with our JV partner AMEC, more than $240 million in DoD-sponsored construction projects, including the two largest hangars in the world.

There is a big step up in resolution with this objective, as expected given its much higher NA. However showing the images at this size online doesn’t really show that, as they have to be reduced in resolution for the website. Below is a crop from the image above, which has also had to be shrunk in resolution).

The images above have been cropped from the original and have not been cleaned up (they still show all the imperfections from the camera sensor – microscopy will show everything on the sensor, even when you think you have cleaned it). Below is a cleaned up image of the Synedra superba diatom slide using 405nm oblique illumination with the Olympus 40x Dplan Apo UV objective.

Since the ray refracted by the optic is farther away, it crosses the axis at a point shifted Δf beyond the other ray's crossing. Increasing the optic's ...