Today I had a look at the diagrams for both classes. The Q is slightly longer at the front so the running plate needs extending. The cabs are different, the boiler mountings are as well. The wheels on the Q are 5ft 1in and the 4 F 5ft 3in is it worth rewheeling? There are minor dimensional differences with the internal measurements of the boiler, how much that translates once the cladding is in place I don't know. They of course hauled tenders that didn't look alike but have the same wheelbase, so even if using a old Airfix tender drive loco it should be possible to make a reasonable looking Q from the 4F model.

We have a Wills Q on a Triang Chassis with Hamblins wheels.  Built probably 40 years ago. Awful tender coupling which requires a Fireman of Commonwealth games long jump standard to get from loco to tender, (on the to do list for 20 years)  Very powerful, better than Bachmann ROD Hornby 28XX etc   smooth, essentially useless like the real thing, it sits in the display cabinet waiting for a chance to perform on a summer Saturday extra off the Southern.....

If you examine the L1, E1 and D1 locos they also show a striking resemblance to the 2P 4-4-0s built by the Midland for much the same reason.

Left: beta-barrel structure and fluorescent core of enhanced GFP (EGFP), a GFP derivate; right: magnification of the fluorescent core, the two-ring chromophore

Golden Arrow do a resin body for the loco and tender to fit the 4F running gear, I have one in my ever growing "to do" pile.

Unfortunately nothing will be happening soon as we are going on holiday tomorrow so work will be put on hold until we get back but I will try to keep anyone who is still interested informed of progress.

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Crownline used to produce a conversion kit many years ago. It eventually became a full loco kit in itself and is now available through PDK.

Nano-Trap system provides fast, reliable, and effective immunoprecipitation of fusion proteins. More than 3,000 peer-reviewed articles have already been published using ChromoTek's Nano-Traps.

If 'looks like' is close enough, three overlays on the cab, spectacle plate and two cab side sheets, moving the front step forward about 2mm, changing the sand box shapes, a capuchon for the chimney, and giving the loco a SR or BR(SR) number does it as far as the loco is concerned.

A pretty minimal 'kit' which can all be done for near nothing from plasticard, and the joy of it is that the necessary parts can all be made off the model with lots of 'cut and try' until correct. Only then does the model get cut as required to allow the restyling parts to go on. A really good first project if nothing like this has been previously attempted.

Today I had a look at the diagrams for both classes. The Q is slightly longer at the front so the running plate needs extending. The cabs are different, the boiler mountings are as well. The wheels on the Q are 5ft 1in and the 4 F 5ft 3in is it worth rewheeling? There are minor dimensional differences with the internal measurements of the boiler, how much that translates once the cladding is in place I don't know. They of course hauled tenders that didn't look alike but have the same wheelbase, so even if using a old Airfix tender drive loco it should be possible to make a reasonable looking Q from the 4F model.

If I was modelling the SR I would have ago at this conversion, one it looks doable and two when I visit the Bluebell and the Q is in steam in it is my favorite of their locos.

Chromotek offers GFP Nanobodies conjugated to beads for immunoprecipitation and unconjugated GFP Nanobodies/VHHs:GFP-Trap Agarose: anti-GFP Nanobody conjugated to agarose beadsGFP-Trap Magnetic Agarose: anti-GFP Nanobody conjugated to magnetic agarose beadsGFP-Trap Magnetic Particles M-270: anti-GFP Nanobody conjugated to magnetic particles M-270 for analysis of very large proteins/complexes.

GFP stands for green fluorescent protein. GFP is a fluorescent protein that can be expressed in vivo. If GFP is exposed to light, it emits a green fluorescent signal. This property has had an enormous impact on cell biology by enabling the imaging of almost any protein, in transcription studies by working as a reporter gene, and in biochemical applications.

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I intend to model the result as 30541, not because it is preserved but because it seems to be the only one of the class which is recorded as running on the S&D, I think on a goods train to Blandford Forum. There may have been others but no-one bothered about them, possibly thinking that they were 4Fs.

If you use the Airfix as a starting point you are still stuck with the Airfix Tender drive which has great traction but in my experience jumps about like an over excited frog and makes the track filthy.   Maybe a Bachmann 3F chassis under the loco would be better?  The Triang 3F is 8ft +8ft 3" so 3" too short but looks al right under the Wills Q if you close your eyes to the magnet in the cab or trim the motor short by using a stack of small Chinese super neo magnets in place of the standard block.

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GFP is a protein and, like other proteins, it can be expressed by living organisms. Once GFP is expressed and properly folded, it shows fluorescent properties. If excited by light in the ultra-violet or blue spectrum, GFP emits green light (for more details please see sections 8 and 9). This property has had an enormous impact on cell biology: GFP and proteins fused to GFP can be detected as GFP works as a fluorescent tag. This has actually made lots of new experiments possible.4. What are GFP-tagged proteins and GFP-fusion proteins.

Endogenous proteins do not contain protein or peptide tags and therefore are sometimes difficult to detect in an assay. One solution that enables easy detection is to genetically fuse protein and peptide tags to the protein of interest. Tagged proteins can be used for purposes such as immunoprecipitation, microscopy, protein purification, Western blotting, protein arrays, etc. Proteins fused to GFP are called GFP-tagged proteins or GFP-fusion proteins. GFP-tagged proteins are often used for fluorescence microscopy, immunoprecipitation, protein purification, and Western blotting.

Hi all, following to a recent visit to the Bluebell Railway I was struck by the resemblance between their Southern Q Class and the LMS 4F.

Many fluorescent proteins are based on the GFP sequence. These fluorescent proteins are genetically engineered and have different properties such as different excitation/emission spectra, photo or pH-stability, folding properties, half-time etc. Here is a rough overview:

You an get really cheap Lima 4F's on that well known auction site, Going usually for a few pounds. I know they are H0 scale, But that should make the wheels nearer the correct size. It would be a good start for the chassis.

GFP and GFP derivate EGFP have a β-barrel structure. In the center of this β-barrel, there are 3 amino acids. These acids and the cyclization and oxidation of their backbone form a two-ring chromophore.

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As the gap width approaches the wavelength of the wave, the distortion becomes even more obvious. This process is known as diffraction. If the diffracted light ...

The two-ring chromophore of GFP absorbs and emits light, e.g., light photons, in the visible green spectrum.The chromophore, actually a two-ring chromophore, of GFP lies in the center of a beta-barrel structure. The two-ring chromophore is formed by oxidation and cyclization of the backbone of 3 amino acids: Threonine 65, Tyrosine 66, and Glycine 67. This process occurs during the folding of the protein and depends on different factors such as pH, temperature, and oxygen concentration.

I was thinking that some find gluing whitemetal easier than soldering etched kits, though soldering whitemetal is my preference

Way back in the 80s Vivian Thompson wrote an article on converting a 4F to a Q in Model Railway Constructor. I am sure someone will tell which edition.

4  Firebox, the Q is much more curvaceous, the 4F very straight especially at the front top corners.  5 wheels, the Q wheels look smaller than the 4F and the splashers are noticably smaller.  Most models I have seen seem to have oversize wheels though the 20mm Airfix are very nearly the right diameter.   The 4F Splashers hide the frames the Q don't and the shape of the frames can be seen behind them.

Yes and no. Bits of the design are very simple indeed - that's Paul Hill's trademark, in a way, because he designs for robustness (look at the way he designs valve gear and motion, for example) rather than finescale.

Hi all, following to a recent visit to the Bluebell Railway I was struck by the resemblance between their Southern Q Class and the LMS 4F.

The Wills/Southeastern Finecast SR Q class loco is reasonably common on sites like eBay, normally in the former with Triang/Hornby chassis at quite modest prices. The Triang/Hornby chassis are quite bullet proof and can lend themselves to be upgraded with MRRS 5 pole motors and or Romford/Markit wheels and might be a bit more reliable than the Airfix mechanisms> I would guess the PDK kit to be a bit superior, but far harder to build

If I was modelling the SR I would have ago at this conversion, one it looks doable and two when I visit the Bluebell and the Q is in steam in it is my favorite of their locos.

Be careful when buying 5 pole XO4 lookalikes. Some are intended for slot cars and go like sh*t off a shovel but consume lots of amps.

Yes and no. Bits of the design are very simple indeed - that's Paul Hill's trademark, in a way, because he designs for robustness (look at the way he designs valve gear and motion, for example) rather than finescale.

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Hi all, following to a recent visit to the Bluebell Railway I was struck by the resemblance between their Southern Q Class and the LMS 4F...

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5 Front overhang, part of this is a longer smokebox, part forward of the smokebox.  The only drawing I have access to at present is very wrong about the shape of the frames at the front but it looks like someone decided to leave enough room for someone to stand on the frames to undo the smokebox lugs, which the 4F does not really have.   6 Boiler side gubbins, the Q has lubricator pipes and an ejector pipe  on the boiler side in addition to the Handrails.  The 4 F has them buried.

I was able to take advantage of one of those 'Lets sell off stuff at stupid prices!' times Rails or Hattons do. This meant the price of the C was reasonable.

The Triang/Hornby chassis are quite bullet proof and can lend themselves to be upgraded with MRRS 5 pole motors and or Romford/Markit wheels and might be a bit more reliable than the Airfix mechanisms>

GFP is an endogenous protein from the jellyfish Aequorea Victoria. It was isolated by Osamu Shimomura in 1962. In 1992, the sequence of GFP was cloned (Douglas Prasher) and Martin Chalfie’s lab expressed the sequence in vivo. Roger Tsien’s lab improved GFP and managed to convert it into a commonly used research tool. In 2008, The Nobel Prize in Chemistry was awarded “for the discovery and development of the green fluorescent protein, GFP.” See Roger Tsien’s Nobel Prize lecture here.

Now looking a lot more like a Maunsell 3500g tender!   The top eyes for the handrails were added to the sides and then sides and ends were epoxied on.  As noted before the fit of parts

I'm pretty sure it was in Model Trains as well, as I wasn't a regular reader of MRC at the time. I wouldn't say it was worth rewheeling as 2 inches of difference is barely noticeable and must be close to the margin of error allowed for wheel wear.

The Wills kits were quite basic and the Southeastern Finecast examples are un-revised and still use the Southeastern Finecast etched brass (tender version) Jinty replacement chassis, which is an updated revised kit with brake gear, older more basic Wills Finecast etched kits still pop up.

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To date I have managed to get hold of photocopies of the Roche series of loco drawings for both the 4F and Q and their tenders. There are obviously some differences between locos and tenders but not enough to put me off having a go at a basic conversion. David has summarised these differences in his post above but I will still have a try.

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Progress of sorts is being made.  Last week's job was to enhance those cylinder bolts.  A jig was made that landed 0.5mm holes on the half etched rivets - so far so good, yet 0.5mm wire when fitted ap

Way back in the 80s Vivian Thompson wrote an article on converting a 4F to a Q in Model Railway Constructor. I am sure someone will tell which edition.

This resemblance has a lot to do with the fact that one of Maunsel's long time assistants (James Clayton) cut his teeth on the Midland Railway.

Visually the differences as I see them are, 1 bigger smokebox door, maybe LN or Schools size?   2 Cab, maybe adapt a schools (or LN) as stated above, 3 Running plate.  The Q has almost no "lip" at the top, very straight sided as is the tender and forms a straight line from loco buffer beam to tender bufferbeam in every photo I have seen.   The 4F has a pronounced overhang of the top surface of the running plate.

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Here's my attempt, as far as it's got at the moment  -  obviously still quite a bit to do.   Old Airfix 4F body with Crownline conversion kit (which included new tender). Loco chassis also replaced

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Hi all, following to a recent visit to the Bluebell Railway I was struck by the resemblance between their Southern Q Class and the LMS 4F.

Thanks to everyone for the replies and suggestions. "Looks like" is always good enough for me, especially if I can try out any changes in advance without damaging the body.

Can GFP antibodies detect YFP, CFP, or other derivatives?That depends on the antibody. Most GFP antibodies also recognize other derivatives, especially if there are just a few amino acid substitutions compared to GFP or EGFP. For example, the GFP-Trap recognizes AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP, S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP K206A, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP (click here for a complete list).Is GFP still fluorescent after fixation?There is no general answer to this question as it depends on the fixation method and procedure.