For clinical research requiring polarized light microscopy and pathology training, these achromat objectives enable transmitted polarized light observation at an affordable cost.

The angle of view is the maximum view a camera is capable of 'seeing' through a lens, expressed in degrees. The choice of focal length is key, with longer lenses offering a narrower angle of view. Digital Camera World has put together another guide to help you understand the relationship between focal length and angle of view.You need to consider sensor size, too. The angle of view can be measured horizontally, vertically and diagonally, but lens manufacturers often list just the diagonal, corner-to-corner angle. Focal length and sensor size affect the maximum angle of view possible – and the angles of view illustrated here are for lenses attached to a full-frame camera.

Designed for low-magnification, macro fluorescence observation, this semi-apochromat objective offers a long working distance, a high NA, and high transmission of 340 nm wavelength light.

These semi-apochromat long-working distance water-dipping objectives for electrophysiology deliver flat images for DIC and fluorescence imaging from the visible range to the near-infrared. Their high NA and low magnification enables bright, precise macro/micro fluorescence imaging for samples such as brain tissue.

Objective lenses are responsible for primary image formation, determining the quality of the image produced and controlling the total magnification and resolution. They can vary greatly in design and quality.

Optimized for multiphoton excitation imaging, these objectives achieve high-resolution 3D imaging through fluorescence detection at a focal point of a large field of view. They enable high-precision imaging of biological specimens to a depth of up to 8 mm for in vivo and transparent samples.

Angle of view is favored by lens and camera manufacturers over field of view, because it doesn't change in relation to the working distance (the distance from the subject to the front element of the lens). And while angle of view can be measured horizontally, vertically or diagonally, manufacturers tend to stick to the latter. To calculate field of view, it helps to know the lens and camera combination's angle of view.

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Offering our highest numerical aperture values, these apochromat objectives are optimized for high-contrast TIRF and super resolution imaging. Achieve wide flatness with the UPLAPO-HR objectives’ high NA, enabling  real-time super resolution imaging of live cells and micro-organelles.

To calculate the angle of view, set your calculator to use degrees before applying the following equation. If you cannot set your calculator to use degrees, you'll have to convert the radian value you're left with into degrees.

These super apochromat objectives provide spherical and chromatic aberration compensation and high transmission from the visible to the near infrared. Using silicone oil or water immersion media, which have refractive indexes closely matching that of live cells, they achieve high-resolution imaging deep in living tissue.

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For example, if I were to say: "I'd suggest using an ultra-wide field of view to frame this landscape," you'd know I'm talking about an ultra-wide angle focal length (35mm equivalent). But if I said, "I'm going to use a 16mm focal length to frame this landscape," you'd have no idea whether I was using a full-frame camera, an APS-C camera providing an equivalent 24mm focal length or if I'd already considered my APS-C camera's crop factor and was in fact using an 8mm lens… Discussing field/angle of view ensures everyone is on the same page, regardless of sensor size.

Designed for clinical research and routine examination in labs using phase contrast illumination, these achromat objectives offer excellent field flatness.

When we talk about field of view or angle of view, most of the time precise calculations aren't required. They're often used to help you choose the right focal length of lens to use for your photography. And it's this very reason why they're used interchangeably, given that they both allow the photographer to draw – more or less – the same conclusions.

Field of view (FoV) is an important concept to understand whether you're a photographer or a filmmaker. When it comes to shooting, there's not only a question of what we're seeing in our image but how much of the scene we're seeing. That's where field of view comes in. However, field of view is closely related to – and very often confused with – angle of view (AoV), so I'm going to bust that myth right here, outline the differences and explain how you can calculate both.

These extended apochromat objectives offer high NA, wide homogenous image flatness, 400 nm to 1000 nm chromatic aberration compensation, and the ability to observe phase contrast. Use them to observe transparent and colorless specimens such as live cells, biological tissues, and microorganisms.

These semi-apochromat objectives enable phase contrast observation while providing a high level of resolution, contrast, and flatness for unstained specimens.

You might also like to read: the exposure triangle explained, how to understand f-stops and our general Photography tips and tricks.

Optimized for polarized light microscopy, these semi-apochromat objectives provide flat images with high transmission up to the near-infrared region of the spectrum. They are designed to minimize internal strain to meet the requirements of polarization, Nomarski DIC, brightfield, and fluorescence applications.

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Designed for clinical research and routine examination work in the laboratory, these achromat objectives provide the level of field flatness required for fluorescence, darkfield, and brightfield observation in transmitted light.

These extended apochromat objectives offers a high numerical aperture (NA), wide homogenous image flatness, and 400 nm to 1000 nm chromatic aberration compensation. They enable high-resolution, bright image capture for a range of applications, including brightfield, fluorescence, and confocal super resolution microscopy.

Calculating field of view becomes more important if you require extreme precision due to working at high magnifications. Deep space and photomicrography are two genres where calculating the best telescopes or the best microscopes field of view is advantageous, allowing you to work out, say, exactly what celestial objects you can fit into your frame.

The meaning of PLANE OF POLARIZATION is the plane in which the magnetic-vibration component of plane-polarized electromagnetic radiation lies.

For relief contrast observation of living cells, including oocytes, in plastic vessels, our universal semi-apochromat objectives feature a long working distance. These also provide high image flatness and high transmission up to the near-infrared region.

For use without a coverslip or cover glass, these objectives prevent image deterioration even under high magnification, making them well suited for blood smear specimens. They also feature extended flatness and high chromatic aberration correction.

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This is a tricky one to get your head around, because the two terms are often used interchangeably. But they are separate concepts and are calculated differently, however, they both convey how focal length and sensor size affect the framing of a scene, allowing you to draw similar conclusions. The key difference is that field of view illustrates the area a particular lens and sensor combination will cover in relation to the subject being photographed. Whereas angle of view is used to illustrate a lens and sensor combination's angular coverage, independently of what's being photographed.

To clean a microscope objective lens, first remove the objective lens and place it on a flat surface with the front lens facing up. Use a blower to remove any particles without touching the lens. Then fold a piece of lens paper into a narrow triangular shape. Moisten the pointed end of the paper with small amount of lens cleaner and place it on the lens. Wipe the lens in a spiral cleaning motion starting from the lens’ center to the edge. Check your work for any remaining residue with an eyepiece or loupe. If needed, repeat this wiping process with a new lens paper until the lens is clean. Important: never wipe a dry lens, and avoid using abrasive or lint cloths and facial or lab tissues. Doing so can scratch the lens surface. Find more tips on objective lens cleaning in our blog post, 6 Tips to Properly Clean Immersion Oil off Your Objectives.

Understand field of view in photography and how it relates to the focal length of the lens and the sensor size – the diagrams below shows you more

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These semi-apochromat and achromat objectives are designed for integrated phase contrast observation of cell cultures. They are used in combination with a pre-centered phase contrast slider (CKX3-SLP), eliminating centering adjustments when changing the objective magnification.

Use the handy cheat sheet above to see this all for yourself. And make sure to keep us bookmarked for more great photography cheat sheets.

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Field of view

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For relief contrast observation of living cells, including oocytes, in plastic vessels using transmitted light, these achromat objectives provide excellent field flatness.

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Microscope objectives come in a range of designs, including apochromat, semi-apochromat, and achromat, among others. Our expansive collection of microscope objectives suits a wide variety of life science applications and observation methods. Explore our selection below to find a microscope objective that meets your needs. You can also use our Objective Finder tool to compare options and locate the ideal microscope objective for your application.

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Calculating the field of view is a little more complicated because a number of variables can alter the equation. Thankfully, you can get around this (and the angle of view equation) easily by using an online field-of-view calculator, like this one from Omni Calculator.

Designed for phase contrast observation of cell cultures in transmitted light, these achromat objectives combine field flatness and easy focusing with cost efficiency. They are well suited for routine microscopy demands.

Many microscopes have several objective lenses that you can rotate the nosepiece to view the specimen at varying magnification powers. Usually, you will find multiple objective lenses on a microscope, consisting of 1.25X to 150X.

For high-performance macro-observation, these apochromat objectives provide sharp, clear, flat images without color shift, achieving high transmission up to the near-infrared region of the spectrum. They perform well for fluorescence, brightfield, and Nomarksi DIC observations.

This semi-apochromat objective series provides flat images and high transmission up to the near-infrared region of the spectrum. Acquiring sharp, clear images without color shift, they offer the desired quality and performance for fluorescence, brightfield, and Nomarksi DIC observations.

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These apochromat objectives are dedicated to Fura-2 imaging that features high transmission of 340 nm wavelength light, which works well for calcium imaging with Fura-2 fluorescent dye. They perform well for fluorescence imaging through UV excitation.

This super-corrected apochromat objective corrects a broad range of color aberrations to provide images that capture fluorescence in the proper location. Delivering a high degree of correction for lateral and axial chromatic aberration in 2D and 3D images, it offers reliability and accuracy for colocalization analysis.

Unsure of what microscope objective is right for you? Use our guide on selecting the right microscope objective to weigh your options.

Field of view is simply the observable area that you can see either through your eyes or an optical device, such as a camera lens or telescope. The official definition according to Wikipedia is "the extent of the observable world that is seen at any given moment".

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What I like about the Omni Calculator is that – providing you enter your sensor's width, height and diagonal – it'll provide horizontal, vertical and diagonal AoV and FoV measurements.

For phase contrast observation of cell cultures, these universal semi-apochromat objectives provide long working distances and flat images with high transmission up to the near-infrared region. They help you achieve clear images of culture specimens regardless of the thickness and material of the vessel.

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Don't confuse field of view with depth of field, which has to do with how much of your observable scene is actually in focus. When we talk about view, it could be seen through your eyes, through the camera's viewfinder, or on a viewing screen. The term refers to the whole coverage of a scene, rather than one fixed focal point.

Enabling tissue culture observation through bottles and dishes, these universal semi-apochromat objectives feature a long working distance and high contrast and resolution. Providing flat images and high transmission up to the NIR region, they are well suited for brightfield, DIC, and fluorescence observation.

The ocular lens is located at the top of the eyepiece tube where you position your eye during observation, while the objective lens is located closer to the sample. The ocular lens generally has a low magnification but works in combination with the objective lens to achieve greater magnification power. It magnifies the magnified image already captured by the objective lens. While the ocular lens focuses purely on magnification, the objective lens performs other functions, such as controlling the overall quality and clarity of the microscope image.

Lauren is a writer, reviewer, and photographer with ten years of experience in the camera industry. She's the former Managing Editor of Digital Camera World, and previously served as Editor of Digital Photographer magazine, Technique editor for PhotoPlus: The Canon Magazine, and Deputy Editor of our sister publication, Digital Camera Magazine. An experienced journalist and freelance photographer, Lauren also has bylines at Tech Radar, Space.com, Canon Europe, PCGamesN, T3, Stuff, and British Airways' in-flight magazine (among others). When she's not testing gear for DCW, she's probably in the kitchen testing yet another new curry recipe or walking in the Cotswolds with her Flat-coated Retriever.

Like angle of view, field of view can be measured horizontally, vertically or diagonally. But in most cases, it's measured horizontally to illustrate the field of view of a landscape-orientation image or vertically to illustrate the field of view of a portrait-orientation image. It's important to note that if you want to work out the horizontal or vertical field of view, you'll need to add the horizontal or vertical angle of view respectively, when following the equation (below).