The BD® Spectrum Viewer is an interactive tool that supports optimized fluorochrome selection, choosing filters and assessing potential spillover when designing flow cytometry panels. The latest and improved version of the tool offers several additional features: ability to view and compare excitation (Ex) and emission (Em) spectra with a broad and growing library of dyes and probes;1 an extensive collection of pre-loaded cytometer configurations;2 a stacked laser view and the ability to save and share your work; and the ability to visualize and analyze fluorochrome emission profiles on spectral flow cytometers3 with a new heat map option to more easily assess areas of overlapping emission. Explore the new BD® Spectrum Viewer below or access the legacy BD® Spectrum Viewer.

A macro lens is not typically designed for capturing distant subjects. Macro lenses are optimized for extreme close-up photography and excel at capturing subjects at a very close range with a high level of detail. They have a short minimum focusing distance and are capable of achieving a 1:1 magnification ratio or greater, which means they can capture subjects at their actual size on the camera sensor.

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However, it's important to note that longer macro lenses tend to be heavier, posing challenges for extended handheld usage, particularly in low-light conditions. If you're pursuing advanced insect photography or prioritizing top-tier image quality and are comfortable with the associated weight and cost, these lenses are a worthy consideration.

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Besides macro and telephoto lenses, photographers often rely on zoom and wide-angle lenses to suit different scenarios and creative preferences. Let's explore some other lens categories and their distinct characteristics.

Choosing between a macro and a telephoto lens depends on your photography goals and the subjects you plan to capture. To help you make an informed decision, let's explore when each type of lens is most suitable.

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Telephoto lenses come in both fixed focal length and variable focal length (zoom) versions. Fixed focal length lenses demand the photographer to maintain a specific distance from the subject, while zoom lenses permit altering this distance. Many zoom lenses also feature autofocus, automatically focusing on the subject. However, some lenses necessitate manual focusing by the photographer.

Macro and telephoto lenses are two distinct types of camera lenses that serve different purposes in photography. Macro lenses are crafted specifically for close-up photography, enabling them to focus far shorter distances than standard lenses. On the other hand, telephoto lenses are characterized by their longer focal lengths, which result in a narrower field of view and the appearance of magnification.

1Fluorochrome excitation and emission data were generated on a spectrophotometer and spectrofluorometer, respectively. Data for immunofluorescence fluorochromes were collected using conjugated antibodies.

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In the end, the choice between a macro lens and a telephoto lens hinges on the specific narratives you wish to craft through your photography. These two lens categories, each with their distinct characteristics, offer pathways to creativity that cater to different subject matter and artistic intentions.

This extended working distance opens up opportunities for creative macro techniques, such as capturing shots through out-of-focus flowers and adding an artistic touch to your compositions.

In this article, we will delve into the fundamental differences between macro and telephoto lenses, exploring their unique features and applications. Let's start!

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Short macro lenses are often employed for casual macro photography excursions or as versatile lenses that can be switched to macro mode when required. They offer convenient storage, effortless portability, and generally cost less. Their compact size also makes them simpler to hold by hand.

Macro lenses can be categorized based on their focal lengths into three groups: short-range(35-60mm), mid-range (90-105mm), and long (150-200mm).

Understanding the advantages and disadvantages of both macro and telephoto lenses is crucial for choosing the appropriate lens for your specific photographic needs. Here's a comparison of the pros and cons of these two types of lenses.

In many zoom lenses, the barrel elongates while rotating, although some lenses maintain a consistent barrel length even during changes in focal length.

3Fluorochrome spectral signatures were generated on the respective instrument platform using recommended set-up protocols. Spectral signatures are dependent on the cytometer configuration and settings used, and, as a result, results on individual instruments may differ from what is depicted herein.

2Collection of pre-loaded configurations includes the following flow cytometers: BD Accuri™, BD FACSAria™ Fusion, BD FACSAria™ III, BD FACSCalibur™, BD FACSCelesta™, BD FACSJazz™, BD FACSLyric™, BD FACSMelody™, BD FACSVerse™, BD FACSymphony™ A1, BD FACSymphony™ A3, BD FACSymphony™ A5, BD FACSymphony™ A5 SE, BD FACSymphony™ S6, BD® LSR II, BD LSRFortessa™, BD LSRFortessa™ X-20.

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In the ever-evolving world of photography, two types of lenses have consistently captured the imagination of enthusiasts and professionals alike: macro and telephoto lenses. These two lens categories cater to distinct yet equally fascinating aspects of photography, allowing photographers to delve into the intricate details of tiny subjects or capture distant scenes with astonishing clarity.

No, telephoto and macro lenses are not the same. They serve different purposes and have distinct characteristics that cater to specific types of photography. Telephoto lenses are used to capture distant subjects with magnification, while macro lenses are used to capture extreme close-ups of small subjects.

Use BD RC to go beyond the fluorochrome spectra and into panel design. The predefined Reagent Selector workflow guides you through the panel design process and provides a customized experience by filtering reagent choices according to your instrument configuration and reagent availability so that you only build a panel that is compatible with your instrument and available for ordering. Create your own custom panels or use one of our pre-optimized multicolor flow cytometry panels. Share the panel with collaborators and save time on the cytometer by translating your panel into a BD FACSDiva™ Software experiment directly in BD RC. Sign up for a free account, configure your cytometer and start designing your panel today at bdresearchcloud.com

Usually, telephoto lenses have a relatively far minimum focusing distance. This means that they aren't suitable for capturing subjects up close, and they're generally not ideal for macro photography. Additionally, telephoto lenses typically offer lower magnification capabilities compared to macro lenses.

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A true macro lens achieves a 1:1 magnification ratio, signifying that the subject appears at life-size on the camera's sensor. It produces a genuine-to-life image of the subject. Additionally, certain macro lenses can push magnification even further, reaching ratios like 2:1 or even as high as 5:1. However, such advanced magnifications are typically found in professional-grade lenses with premium pricing.

Many modern zoom lenses offer a feature called "macro mode" or "close-up mode," allowing you to focus on subjects closer to usual. While these modes can provide some level of magnification and are convenient for capturing a bit closer subjects, they might not provide the same level of magnification and detail as dedicated macro lenses.

However, a significant limitation of short macro lenses is their reduced working distance. Additionally, under specific lighting conditions, your head or camera might cast unwanted shadows on the subject. Furthermore, shorter lenses tend to produce less appealing bokeh compared to longer focal lengths.

Mid-range macro lenses offer a greater working distance advantage over short ones. This distance allows for capturing macro shots without getting too close to subjects. They present an excellent choice for flower photography, particularly in the realm of abstract flower photography.

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A zoom lens is a camera lens designed to alter the focal length and, consequently, the camera's angle of view. This characteristic permits you to magnify your subject (zoom in) or encompass a broader scene (zoom out) without requiring physical proximity adjustments. Zoom lenses encompass an assortment of focal lengths within a singular lens, rendering them adaptable instruments suitable for a diverse array of photography scenarios.

One of the standout features of macro lenses is their notably shorter minimum focusing distance. This allows you to get exceptionally close to your subject from the start. Furthermore, macro lenses tend to offer a wider maximum aperture compared to telephoto lenses, which offer greater control over the depth of field. This, coupled with the 1:1 magnification, enables superior close-up shots of minuscule portions of your subject.

A wide-angle lens is a type of camera lens that has a shorter focal length than standard lenses, allowing it to capture a broader scene within the frame. This lens type is particularly useful for capturing expansive landscapes, architectural structures, and scenes where you want to emphasize space and depth.

Longer macro lenses consistently deliver remarkable image quality. They also excel in providing the most generous working distance, a crucial aspect of insect photography.

A telephoto lens is designed to bring subjects closer in appearance than their actual distance. These lenses are generally categorized into two primary groups: medium telephoto (around 70 mm to 200 mm) and super telephoto (300 mm or greater).

However, while offering more working distance, mid-range lenses might not suffice in scenarios requiring even greater separation. For highly sensitive or delicate subjects, lenses with longer working distances, like the long macro lenses, could be more suitable.

Macro lenses are purpose-built for capturing incredibly close-up and detailed photographs, often used to depict subjects like insects or flowers with intricate textures.