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The 10x objective lens typically has a numerical aperture of 0.25 and a focal length of 4mm. The field of view at 10x is around 2mm across.

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These lower magnification objectives provide a wide field of view which is useful for scanning slides and locating specimens. Resolution and fine detail are low, but the overview of the sample is clear.

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It has a very high 1.25 numerical aperture and a short focal length of 0.2mm. At 100x the field of view is only 0.2mm wide.

Start with the lowest power 4x or 10x objective to locate the specimen. Then increase magnification to observe specific structures. Use the 100x oil immersion lens for the finest details.

Anti-reflective coatings have vastly improved in recent years. There are now hundreds of different types for each kind of lifestyle and patient need.

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Some microscopes may also have specialized oil immersion objectives like 60x or 150x. Like the 100x lens, these objectives require immersion oil to reach their maximum NA and magnifying power. Immersion oils are specially formulated to have specific refractive indexes matched to the lens.

The process of creating premium anti-reflective coatings requires substantial technology, and looks something like this:

The 4x scanning objective has a numerical aperture of 0.10 and a focal length of 16mm. It has a field of view of 5mm across.

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To achieve the full NA of 1.25, immersion oil is placed between the slide and the 100x objective. This prevents refraction and loss of resolution from the air-glass interface.

Plan apochromat objectives are high-quality optimized lenses that reduce optical aberrations and provide flat, sharp images. Fluorite lens elements allow a wider spectrum of light throughput. These advanced objectives paired with oil immersion provide the clearest high-magnification views.

The field of view at 40x is 0.5mm across – much smaller than the lower power lenses but still enough to see most cell details. 40x is commonly used for studying cell structures.

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Courtney Dryer, OD, received her doctor of optometry degree from Southern College of Optometry in 2011. She is the owner of Autarchic Spec Shop in Charlotte, NC. She has worked with Eyes on Eyecare since its founding and regularly contributes to optometric publications and vision care websites.

The high NA paired with oil immersion allows the 100x objective to see fine subcellular details like organelles and chromosomes. It is used to study cellular structure and mitosis.

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The objective lenses on most compound microscopes typically come in 4x, 10x, 40x, and 100x magnifications. Some microscopes may also have a 60x or 150x objective.

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Lower magnification gives a wide field of view to find and identify organisms. Medium magnifications like 400x are ideal for studying cell structure. 1000x is used for finer details like organelles.