I-beam - beam shape
Usually the working distance (WD) refers the distance from the front lens element of the objective to the observed object when the object is in sharp focus. Objective lenses with long working distance are needed for many scientific research applications such as atom trapping and analyzing fluid samples that require putting an object in a chamber. The resolution of a microscopy system can be significantly affected if the observed object is not placed on the designed object plane, especially for an objective with high NA.
Objective lenses are used in microscopy systems for a range of scientific research, industrial, and general lab applications. A microscope objective is typically composed of multiple lens elements and located closest to the object. There are so many types of microscope objectives available, choosing the right objective can help you produce good quality images at a reasonable cost. When choosing a microscope objective, we will need to consider a number of factors including conjugate distance, numerical aperture (NA), magnification, working distance, immersion medium, cover glass thickness, and optical aberration corrections. In this article, we will discuss how to choose the right microscope objective.
A dry objective is designed to work with the air medium between the specimen and the objective lens, while an immersion objective requires a liquid medium to occupy the space between the object and the front element of the objective for enabling a high NA and high resolution. Figure 4 shows the oil immersion objective, which can collect more light (i.e., have a higher NA) compared to a dry objective.
The most important parameter of a microscope objective is the numerical aperture (NA). NA measures the microscope objective’s ability to gather light and determines the resolution of a microscopy system.
Microscopelabeled
NA is commonly expressed as NA = n × sinθa where θa is the maximum 1/2 acceptance angle of the objective, and n is the index of refraction of the immersion medium. The limit of resolution of a microscope objective refers to its ability to distinguish two closely spaced Airy disks. Resolution (r) = λ/(2NA) Where r is resolution (the smallest resolvable distance between two objects), and λ is the imaging wavelength. The higher the NA, the better the objective resolution.
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The latest version of ViaTouch can be combined with the optional TransMount Image Transmission Module to turn a connected mobile device into a wireless monitor that can also be used to adjust aperture, ISO, shutter speed and gimbal movement settings. It also unlocks precise object tracking as well as the sync motion feature to control gimbal movement using your phone’s motion sensor.
Low powerobjective microscopefunction
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Types ofmicroscopeobjectives
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SO offers a wide range of objective designs, which provide various degrees of optical aberration corrections for supporting different needs, such as achromatic objectives (the cheaper objectives) for laboratory microscope applications and long working distance apochromats (expensive objectives) for biological and scientific research applications. We can help you choose or design a properly corrected objective lens for meeting your application requirements.
Featuring an improved motor unit and enhanced stabilisation algorithm, the Zhiyun WEEBIL-S 3-axis motorised gimbal is a remarkably efficient and compact device suitable for a variety of Mirrorless and DSLR cameras and lenses. An upgraded auto-tune feature makes setup a breeze, and a remarkable fourteen hours of battery life is possible with the latest WEEBIL gimbal.
Function of condenserin microscope
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The TransMount Quick Setup Adapter from Zhiyun-Tech is a connector which facilitates the fast attachment of mini tripods and monopods to the WEEBILL-S gimbal. It comprises two components: one for the tripod/monopod and the other for the gimbal's tripod mount, allowing for simple mutual connection. For removal, simply pull on the collar. The adapter obviates the need to repeatedly unscrew/screw a tripod/monopod and additionally features a 1/4"-20 male/female design, enabling its separate utilization with different accessories e.g. monitors.
The best thing about the Zhiyun WEEBIL S is the versatility for different sized productions. It can be stripped-back for run-and-gun documentary filmmakers or built up for a bigger production crew with the range of accessories. The TransMount Image Transmission Module adds wireless Full HD low latency monitoring with a 100m range, while the Wireless Remote Controller delivers precision motion control as well as real-time camera parameter control.
Objective lenses are used to magnify an image. In addition to numerical aperture, magnification is also an important parameter. The objective magnification typically ranges from 4X to 100X. As the image sensor size or eye observed area is fixed, the field of view of a microscopy system changes with the magnification of the objective lens. Typically a lower magnification objective lens will have a larger field of view and lower resolution, and a higher magnification objective lens will have a smaller field of view and higher resolution. The diameter of the FOV can be calculated by using the following formula: FOV= FN/Mag The field number (FN) in microscopy is defined as the diameter of the area in the image plane that can be observed through the eyepiece or image sensor.
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Using a quick release plate that is compatible with both Manfrotto and Arca-Swiss style tripod heads means you can easily swap your camera between gimbal setups and sticks in a couple of moves. This gives you the flexibility to change setups quickly and securely for a significantly faster workflow on set.
MicroscopeObjectives magnification
The WEEBIL-S is powered by the same 2600mAh 18650 Zhiyun batteries but now the same batteries deliver up to 14 hours of operation. The batteries are removable and additional units can be purchased, although the Zhiyun brand are recommended due to their slightly shorter size.
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Infinity-corrected objectives are ideal for research-grade biomedical industrial applications especially when additional components (such as filters, dichroic mirrors, polarizers) are needed in the microscopy system. Adding optical plate components in the infinity space (shown in the Fig.2 labelled as “Parallel Optical Path) between the infinity-corrected objective and tube lens will not introduce spherical aberration, or change the objective’s working distance.
Aimed squarely at video shooters on-the-go that want incredibly versatile performance without carrying a massive rig, the WEEBIL-S weighs in at only 926 without the tripod or batteries installed and has integrated locks on each axis for safe and secure transport.The improved motors deliver a dramatic 300% improvement in motor torque which translates to a 50% increase in response speed.
Objectivelensmicroscopefunction
A large dial on the side of the WEEBIL-S can be used to control either focus or zoom when combined with the Zhiyun motorised servo motor. Some cameras will also support electronic focus control which can be set in the menu of the gimbal.
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The optical aberration corrections determine the optical performance of an objective lens. According to the degrees of the aberration corrections, objective lenses are typically classified into five basic types: Achromat, Plan Achromat, Plan Fluorite (Plan Semi-Apochromat), Plan Apochromat, and Super Apochromat. Choosing an objective with a proper aberration correction level will help you build a microscopy system at a reasonable cost.
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The WEEBILL-S offers filmmakers considerable flexibility with its multiple modes, such as the PF mode, which stabilizes the camera's tilt and roll motions, thus ensuring smooth circling shots. The all-follow mode is similar but employs both pan and tilt function, while the all lock mode maintains the camera facing in a single direction.
Microscopeparts
The most common immersion media are air, water, oil, and silicone. Choosing the appropriate objective designed for your immersion medium will result in higher resolution images.
Stagemicroscopefunction
The Zhiyun-Tech TransMount Weebill-S Handheld Tripod is an add-on or replacement mini tripod that can be either used as a standing support or a handle for Sling Mode wkth the Weebill-S gimbal.
Many objective lenses are corrected for infinite conjugate distance, while others are designed for finite conjugate distance applications. Compared to infinite conjugate objectives which need a secondary lens (also called tube lens), a finite conjugate objective can generate an image of a specimen by itself. A finite conjugate objective, as shown in Figure 1, is a good, economical choice for a simple microscopy system.
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noun A lens or combination of lenses that enlarges the image of an object. from The Century Dictionary. noun In optics, a convex lens: so called because objects ...
The WEEBIL-S also offers sophisticated operation modes for unique shots. The POV mode follows an operator’s roll movements, creating a point-of-view look. The Vortex mode points the camera up and rotates on the pan axis for stunning 360° barrel rolls, while the Go mode increases sensitivity and motor responsiveness, making it easier to follow fast moving subjects.
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The WEEBIL-S has a removable tripod base that folds up to become a handle. It can be attached at the base like a traditional gimbal, or at the back of the gimbal facing upwards to create a underslung-style grip. This makes is easier to capture super-low angle footage and to transition from low to high shots smoothly, allowing you to capture crane-like jib shots.