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In comparison, SEMs are capable of much greater magnification and higher resolution. The most sophisticated SEMs can achieve magnifications of around 100,000 x and sub-nanometer resolutions, which are capable of imaging viruses (which are between 30 and 250 nm) and molecules such as proteins (10 nm) and glucose (1 nm).

SEMs typically feature three types of detectors, each of which captures a different signal coming from the sample: A Secondary Electron Detector (SED), a Back-Scattered Electron Detector (BSED), and an Energy Dispersive Spectrum Detector (EDS).

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A magnifier (i.e. magnifying lens) is a lens or combination of lenses used to magnify (or enlarge) an object. Magnifiers and magnifying glasses enable people to magnify a variety of objects (e.g. books, newspapers, fine print, stamps, coins, antiques, art, jewelry, gemstones, foliage, insects, and rocks). The magnifying glass was invented in 1250 by Roger Bacon. Today, over 750 years later, magnifiers and magnifying glasses are more popular than ever, assisting people with hobbies and crafts and also helping people with low vision, macular degeneration or other vision conditions to see better.When it comes to magnifiers, most people probably think of the classic hand-held magnifying glass used by Sherlock Holmes. However magnifiers come in a wide variety of shapes, sizes and magnifications. At Blaxall Optics Vision, we stock over 250 different magnifiers! In addition to Hand-Held Magnifiers, we also carry Hands-Free Magnifiers, Lighted Magnifiers, Page, Bar & Dome Magnifiers, Loupes, Linen Testers & Stand Magnifiers, Pocket Magnifiers, Reading, Specialty Glasses & Monoculars, Magnifying Mirrors, Portable Microscopes, Opera Glasses & Binoculars,  Each of the magnifiers in these categories meets different magnification needs. In order to select the best type of magnifier to meet your individual magnification needs, please consider the following information. First, we need to discuss and understand three main points -- lens size, magnification, and focal length -- on how magnifiers work. Lens Size The lens size is the optical area of the lens, generally measured in diameters for round lenses or in length and width for rectangular or square lenses. The lens size is a very important component of a magnifier because the lens size needs to be large enough or small enough to use the magnifier effectively. (Some people want the largest lens size possible to see as much of a subject as possible. Other people want a small lens size to enable them to keep the magnifier in their pocket, purse or handbag.) In any case, purchase the magnifier to meet your particular needs. Magnification   Magnification is the process of enlarging an object in visual size, normally through an optical lens. It is the ratio between the apparent size and the true size of the viewed object behind the lens. If an object behind the lens appears ten times larger than its true size, and the object is in focus (i.e. at the proper focal length), the magnification is 10x. Another term used as a measurement of optical power, but different from magnification is diopter. Diopter refers to the optical power (or strength) of the magnifying lens. It is the strength of the magnifying lens measured at one meter. Four diopters (4.0D) represent a 100% increase in magnification or 1x power magnification. A 20 diopter (20.0D) measurement is approximately equal to 5x power magnification. A rough formula to convert from magnification power to diopters is to multiply the magnification power by four. Focal Length Focal length is the distance from the magnifying lens to the object behind the lens when the object is in focus.  The stronger the optical power of the magnifying lens, the shorter the required distance between the magnifier and the object behind the lens.  If you are observing jewelry through a 10x loupe, both the gem and your eye need to be very close to the loupe. However, if you are able to read a book with a 2x hand-held magnifier, the magnifier can be several inches away from the book and your eyes can be several inches away from the 2x hand-held magnifier. Relationship of Lens Size to Magnification Please keep in mind, as the lens size (diameter) of the magnifier lens increases, the magnification power of the magnifier decreases. As the lens size of the magnifier lens decreases, the magnification power of the magnifier increases. This is a result of the amount of curvature in the magnifier (lens). Magnification power is a result of the amount of curvature in the magnifier lens. As the lens size increases, the amount of curvature in the lens decreases, resulting in lower magnification power of the lens. As the lens size decreases, the amount of curvature in the lens increases, resulting in higher magnification power of the lens.  This is why higher magnification lenses are generally smaller in size than lower magnification lenses.Field of View The end result of magnification is to provide you with a magnified “field of view.” The field of view is the area of magnification you see through the lens. Generally, the larger the diameter (or lens area for rectangular or square lenses) of the lens the larger the field of view of the lens. However, not all magnifiers are the same and some have larger fields of view than others. Not all manufacturers identify the field of view as a specification of the magnifier. Types of Lenses Along with understanding the lens size, magnification and focal length, it is important to purchase the appropriate type of material or composition of the optical lens to meet your needs. Magnifier and magnifying glass optical lenses can be made of glass or acrylic (plastic) materials. Each type of lens has advantages and disadvantages when compared to the other. Generally, glass lenses allow more light to be transmitted through the lens and usually have very clear optical qualities. It is hard, but not impossible, to scratch a glass lens. A glass lens is generally heavier than an acrylic lens of the same size.

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In this post, we examine two key methods: optical microscopy and electron microscopy, revealing the benefits each technique brings, and explaining both their differing application areas and operation.

Optical microscopy is an ideal method for general inspection purposes, illuminating and producing a magnified image of a specimen. The layout between optical microscopes varies, depending on the application, but generally includes a converging lens (for magnification) and a concave mirror (to aid illumination). The sample is placed on a stage and the resultant image is viewed through an eyepiece.

Optical microscopy definition: Optical microscopes use a beam of light, ranging from 400nm to 650nm in wavelength, allowing the observer to analyze the effect of light as it is applied to a specimen.

Diopter refers to the curvature of the lens. As the diopter increases, the lens become thicker and the curvature greater. As the curvature increases, light rays are redirected to fill a greater portion of the viewer’s retina which makes the object look bigger.

Magnifying glass

SEM definition: SEMs scan a focused beam of electrons across the surface of a sample, where electromagnets are used to focus the negatively charged electrons. The interaction of the electron beam with the surface of the sample affects the images received. The electrons coming out of the sample are used to create a detailed image and reveal information including the texture (morphology), chemical composition, crystalline structure, and material orientation.

However, SEMs are gaining ground in this area with many Desktop SEMs bridging the gap between optical microscopes and ultra-high resolution SEMs. The unique optical navigation camera displays a view of the entire sample and allows the user to move to any spot on the sample with just a single click. The proprietary venting/loading mechanism supports the highest throughput even for large samples up to 100mm x 100mm ensuring a time-to-image of less than 60 seconds.

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Optical microscopy and electron microscopy both have advantages and disadvantages. SEMs are superior in terms of resolving power and depth of focus. However, optical microscopes are generally easier and quicker to use. As a result, many use a combination of both imaging tools where an optical microscope is used to detect gross defects and SEMs can observe those defects in more detail while observing micro-defects that are not visible using optical microscopy. This two-phase approach combines the benefits associated with each inspection method and provides the customer with a more detailed inspection in less time.

In contrast, SEMs can create a three-dimensional appearance of the specimen image. This is because of the method in which the data is obtained, where a fine electron beam is scanned over the surface and the detected secondary electrons form an image with a high depth of focus.

SEMs are typically larger and operate in a vacuum, which can increase the time to image a sample. Plus, the resulting image is grey-scaled.

Advantages of HeNe lasers: High coherence length. This is very typical to most gas lasers. In case of HeNe, the coherence length varies from 20 cm (for ...

At Thermo Fisher Scientific, we have decades of cross-sector experience, helping industrial and research users get the best images of the micro world. If you could like to find out more about the best inspection tools for your application, click here to speak to one of our expert teams today.

There is one major difference between optical microscopy and electron microscopy – the beam applied to the sample. This simple fact has major repercussions on the components and operation of each microscope, as well as its applications.

There is more than one formula to converts the diopter of a lens into its power, we as a company use the common formula: Magnification = (Diopter / 4) +1

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Acrylic lenses are used as an alternative to glass lenses. They are usually very lightweight compared to glass lenses. Acrylic is the common name for Poly(methyl methacrylate) (PMMA), a thermoplastic or transparent plastic, created in 1928. Its composition normally prevents breakage and shattering and it is normally scratch-resistant. With modern technology, it has become possible to produce magnifiers with exceptional optical qualities using an acrylic lens. A majority of today’s magnifier lenses come with a high quality acrylic lens

Therefore, as the magnification of the microscope is increased, the field of view will decrease proportionally and vice versa as an increase in magnification by ...

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Since secondary electrons interact primarily with the sample surface and have a large reflection angle, the SED provides detailed topographical information. Back-scattered electrons penetrate further into the material and have a smaller reflection angle, so the BSED provides both basic topographical and basic compositional information. The EDS provides detailed chemical compositional information.

NB:  As you increase the magnification the lens size effectively decreases. It is not possible to get a high power lens in a large diameter. Opticical combinations are a compromise for  the “ideal” magnifier.

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Looking at a standard optical microscopy definition, the resolving power of these systems is directly influenced by the wavelength of the imaging beam, which gives SEMs a distinct advantage. Because optical microscopes are limited to the wavelengths of visible light, they can only offer limited magnification (around 1,500 x) and cannot go beyond around 200 nm resolution laterally and 600 to 700 nm axially.

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The human eye is a powerful visual tool but it does not have the resolution to bring microscopic images into focus. This is where microscopy can help us understand everything from pandemic-inducing viruses to the manufacture of increasingly miniaturized electronics, revealing the large impact the smallest detail can have on our day-to-day lives and future endeavors.

This is because, for the optical microscope, the depth of focus is the distance above and below the image plane over which the image appears in focus. As the magnification increases in the optical microscope, the depth of focus decreases.

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Because of the geometry of the imaging system, scanning electron microscopes have a much greater depth of field than optical microscopes, where the whole specimen can be in focus.

Optical microscopes are easy to use, where samples can be analyzed in air or water and the resulting images are in natural color.

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UV/VIS dichroic mirrors have special multilayer dielectric coatings that enable reflectance of a primary UV laser wavelength with good broadband VIS/NIR ...

Mar 29, 2016 — Field of view is a lens characteristic that is directly related to the focal length of your lens and determines how much you can see with a ...

Powers refers to how much larger an object is made to look through a magnifying lens. Power is typically indicated by an X such 2X or 4X.

LENS meaning: 1 : a clear curved piece of glass or plastic that is used in eyeglasses, cameras, telescopes, etc., to make things look clearer, smaller, ...