A high power or compound microscope achieves higher levels of magnification than a stereo or low power microscope. It is used to view smaller specimens such as cell structures which cannot be seen at lower levels of magnification. Essentially, a compound microscope consists of structural and optical components. However, within these two basic systems, there are some essential components that every microscopist should know and understand. These key microscope parts are illustrated and explained below.

Nosepiecemicroscopefunction

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Body tubemicroscopefunction

Stage is where the specimen to be viewed is placed. A mechanical stage is used when working at higher magnifications where delicate movements of the specimen slide are required.

Diopter adjustmentmicroscopefunction

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Stage Clips are used when there is no mechanical stage. The viewer is required to move the slide manually to view different sections of the specimen.

Eyepiece of microscope definitionpdf

Objective Lenses are the primary optical lenses on a microscope. They range from 4x-100x and typically, include, three, four or five on lens on most microscopes. Objectives can be forward or rear-facing.

Coarse and Fine Focus knobs are used to focus the microscope. Increasingly, they are coaxial knobs - that is to say they are built on the same axis with the fine focus knob on the outside. Coaxial focus knobs are more convenient since the viewer does not have to grope for a different knob.

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Nosepiece houses the objectives. The objectives are exposed and are mounted on a rotating turret so that different objectives can be conveniently selected. Standard objectives include 4x, 10x, 40x and 100x although different power objectives are available.

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Objective lensmicroscopefunction

Eyepiece or Ocular is what you look through at the top of the microscope. Typically, standard eyepieces have a magnifying power of 10x. Optional eyepieces of varying powers are available, typically from 5x-30x.

Draw tubemicroscopefunction

Armmicroscopefunction

Eyepiece Tube holds the eyepieces in place above the objective lens. Binocular microscope heads typically incorporate a diopter adjustment ring that allows for the possible inconsistencies of our eyesight in one or both eyes. The monocular (single eye usage) microscope does not need a diopter. Binocular microscopes also swivel (Interpupillary Adjustment) to allow for different distances between the eyes of different individuals.

Condenser is used to collect and focus the light from the illuminator on to the specimen. It is located under the stage often in conjunction with an iris diaphragm.

Fiber optic collimators are used to either coupling light from free space into an optical fiber or collimating light from a fiber to form a collimated (parallel) optical beam. Fiber collimators are key components with numerous applications. For example, in spectroscopy, a fiber collimator can collect light in a narrow field of view into a fiber which is in turn connected to a spectrometer. In another example one fiber collimator is connected to a light source and the collimated beam passes through a cuvette. On the other side of cuvette a second fiber collimator collects light and sends it to the spectrometer.

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Coarse adjustmentmicroscopefunction

Illuminator is the light source for a microscope, typically located in the base of the microscope. Most light microscopes use low voltage, halogen bulbs with continuous variable lighting control located within the base.

Fiber optic collimators are used to either coupling light from free space into an optical fiber or collimating light from a fiber to form a collimated (parallel) optical beam.

For example 10x/22mm: The "field of view" in a microscope eyepiece is what we call with telescope eyepieces "field stop diameter". I understand ...

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Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen.

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PERFORMANCE SPECIFICATIONS (Click here to download datasheet)Part NumberFocal Length (mm)Clear Aperture (mm)F# / NALens MaterialWavelength RangeConnectorFOC-010-006-V1061.7 / 0.29BK7350nm-2000nmSMAFOC-010-006-U1061.7/0.29UV fused silica185nm-2100nmSMAFOC-050-023-U50231.7/0.29UV fused silica185nm-2100nmSMA The full field of view (FOV) or full divergence angle can be calculated as FOV = 2atan(D/2f) where D is the fiber core diameter and f is the focal length of the lens. Alternatively, the linear field of view on an object placed a distance L away from the collimator is D(L/f).

To maximize transmission wavelength range Mightex fiber collimators feature a single BK7 lens without optical coating. In the UV collimators, a UV fused silica lens is used instead. The collimators have a stainless housing for maximum durability. Fiber patchcords with fiber core diameter of up to 1500µm can be connected to the fiber collimator through the SMA connector. The M12x0.5 external thread or the 13mm-diameter barrel can be used to mount the fiber collimators in a system setup.

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