The main function of an iris diaphragm of a microscope is to control the amount of light that reaches the specimen. This light comes from the microscope’s light source, and is gathered by the condenser, before being regulated by the diaphragm, then passing through the specimen.

Light microscopes are made up of several important mechanical and optical components that all work together to make it function as efficiently as possible. One of these components is the iris diaphragm.

Note: Sometimes, the iris diaphragm of a microscope is located within the condenser, in which case it’s called an Abbe condenser. If this is the case for your microscope, you need to find the diaphragm control mechanism on the condenser.

Here is a guide on what the microscope’s iris diaphragm is, what it can do, and how it works, as well as other applications that make use of iris diaphragms.

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From the definition of a diaphragm, an iris diaphragm is a composite type of diaphragm with adjustable or variable opening size. The name is derived from the part of the human eye aptly called the iris, which can dilate and constrict in relation to the size of the pupil.

The iris diaphragm only works in controlling the width of the light beam passing through to the specimen, thereby determining how much of the specimen is being illuminated.

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The use of a diaphragm in controlling illumination and thereby regulating the contrast is especially important in intermediate and high magnifications of a specimen under the microscope.

This is because at higher magnification levels, less light passes through, and as such, the diaphragm needs to have a wider opening to accommodate more light.

Interestingly, however, while the goal in microscopy is to make the image as clear as possible, photography often intentionally manipulates the diaphragm to create artful out of focus spots called Bokeh. There are even specially shaped diaphragms that are designed to achieve certain imagery effects.

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In a microscope, an iris diaphragm is an important component that directly influences the amount of illumination, focus, and contrast of the magnified specimen image.

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What’s important to remember is that the microscope’s iris diaphragm is not what directly determines the intensity of the light, and therefore brightness of the image. This is largely dependent on the intensity of the light source, and the setting of the condenser.

Iris diaphragms can be made of anywhere from two to twenty blades, with many microscope iris diaphragms consisting of five to ten blades. Curved blades make for a perfectly circular opening, while straight blades create a more polygonal shape.

A diaphragm is defined as an opaque structure with a circular opening, called aperture, at the center, which is used to control the amount of light that passes through one point to another. The diaphragm is often referred to as an aperture stop, flare stop, or field stop.

As explained above, the microscope’s iris diaphragm controls the illumination and contrast of the magnified image of the specimen. The wider the diaphragm’s aperture, the higher the illumination and the lower the contrast, and vice versa.

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Other forms include a Zeiss rotating diaphragm, which is a circular plate with several apertures of varying sizes. Another is the Waterhouse diaphragm, which is a set of interchangeable diaphragms made of brass strips.

To a lesser extent, the microscope’s iris diaphragm also influences how focused the specimen image is, and how much the degree of contrast is not only between the specimen and the background, but also within the specimen itself.

There are many forms of iris diaphragms, the most common (and most modern) one being a set of metal or plastic blades or leaves shaped into a circle, where controlling the blades affect the size of the aperture.

As such, diaphragms typically feature variable sized apertures. These adjustable diaphragms are called iris diaphragms or irises.

Apart from microscopy, diaphragms have plenty of uses in a variety of other optical instruments, including cameras. As with microscopy, the basic principles of a diaphragm is the same in photography.

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The diaphragm is located between a light source and a lens, along the optical axis of the lens system, in order for it to regulate the amount of light coming from the light source and passing through the lens. The size of the diaphragm’s aperture is what determines the amount of light.

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The general rule is, the diaphragm’s aperture size is directly proportional to illumination, and conversely proportional to contrast, while the aperture shape is directly proportional to focus. Below is a more detailed explanation of how it works:

Therefore, the iris diaphragm should be wide enough so that the image is not too dark, but not too wide as to wash out the details of the specimen being viewed.

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Increasing the amount of light passing through by widening the aperture of the microscope’s iris diaphragm will increase the illumination of the specimen, making for a brighter image. However, this lessens the amount of contrast on the image, since the light is not as concentrated.

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The more number of blades and the rounder the opening is, the better, more focused, and higher contrast the resulting image is. As such, these iris diaphragms are more expensive, and can be found on more sophisticated devices.

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You can adjust the diaphragm by turning it clockwise to close it, or counterclockwise to open it. Only open the iris diaphragm of the microscope to a point where the light passing through barely extends beyond the microscope’s field of view.

The diaphragm can be found near the bottom of the microscope, above the light source and the condenser, and below the specimen stage. This can be controlled through a mechanical lever, or with a dial fitted on the diaphragm.