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f-number formula
Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform in both prokaryotes and eukaryotes. The short isoform creates significant background fluorescence that biases the outcome of expression studies. In this study, we identified the short protein isoform, traced its origin, and determined the extent of the issue within the family of red fluorescent protein. Our analysis showed that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. We provided a re-engineered mCherry variant that lacks background expression as an improved tool for imaging and protein expression studies.
Numerical aperture formula
To keep the object focussed, the distance of lens and sensor must be increased. This is equivalent with adding distance rings reagrding the infinity position of the lens. As result the images gets darker the field of view gets smaller , the therefore the magnification changes.
numerical aperturetof-number calculator
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The primary function of a microscope is to study biological specimens. A microscope solely functions on two concepts – magnification and resolution.
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f-number calculator
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MH-M was employed by the company United Scientists CORE (Limited). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
F number to naformula
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Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include ...
Numerical aperture of optical fiber
where working F-number or effective F-number. image side numerical apertur, the magnification for this focus distance and is the pupil magnification.
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Numerical aperture
In Type II cut BBO crystals, the crystal is oriented in such a way that the interacting photons have perpendicular polarization directions. This contrasts with ...
The F-number is only defined for objects at infinite distance. The sensor has according the definition of the focal length exactly the distance “focal loength” from the principal plane when the lens is focussed to infinity.
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35 years later, that successful ball bearing design is now part of a larger family of inventive products and services related to linear motion technologies.
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Keywords: alternative translation initiation site; fluorescent protein; fusion protein; gene expression; mCherry; reporter gene.
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