C-mountlens

The LibreTexts libraries are Powered by NICE CXone Expert and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. Legal. Accessibility Statement For more information contact us at info@libretexts.org.

M42mount

A lens adapter allows you to attach a different brand of lens to your camera body, letting you use an otherwise incompatible lens with your camera.

Experiment with new lens combinations and pair any Canon (EF) mount lens with your C-mount camera.Our EF to C-Mount Camera Adapter is manual so most automatic functions – aperture, autofocus, AE metering and image stabilization – must be adjusted manually. Exposure settings need to be set manually too.

Csmount

Shop with confidence knowing you can return products within 30 days of purchase.Sale items are non-refundable unless faulty.

Nikon Fmount

Phase-contrast microscopy allows the visualization of living cells in their natural state with high contrast and high resolution. This tool works best with a thin specimen and is not ideal for a thick specimen. Phase-contrast images have a characteristic grey background with light and dark features found across the sample. One disadvantage of phase-contrast microscopy is halo formation called halo-light ring.

If you have an old second hand lens and you are unsure of the camera it was made for, take all the known details of the lens and search the web.

C mount1 32 un 2b

In summary, attach your lens to the adapter, and then your adapter to your camera body. Put all settings on your lens and camera into manual mode, and select ‘shoot without lens’ in your camera settings.

There are countless creative experiments to be explored by mixing different brands of lenses with your camera body. Lens adapters can also help your existing collection of lenses go further with any new cameras you collect. So you can invest in a new camera and make the most of your favourite lenses.

Telecentriclens

A phase ring in condenser allows a cylinder of light to pass through it while still in phase. Unaltered light hits the phase ring in the lens and is excluded. Light that is slightly altered by passing through a different refractive index is allowed to pass through. Light passing through cellular structures, such as chromosomes or mitochondria is retarded because they have a higher refractive index than the surrounding medium. Elements of lower refractive index advance the wave. Much of the background light is removed and light that constructively or destructively interfered is let through with enhanced contrast.

Lens mount

Phase-contrast microscopy is a method of manipulating light paths through the use of strategically placed rings in order to illuminate transparent objects. Dutch physicist Fritz Zernike developed the technique in the 1930s; for his efforts he was awarded the Nobel Prize in 1953.

Urth acknowledges the Traditional Custodians of this land, the Arakwal people of Bundjalung Country where we live and work. We pay our deep respect to past, present and emerging Bundjalung storytellers, artists, stewards and their deep connection to Country.

Phase-contrast microscopy visualizes differences in the refractive indexes of different parts of a specimen relative to unaltered light.

Partnering with a local Australian archival framer, Urth Art prints are framed with sustainably sourced timber to display the artwork, without damaging the environment. And to bring the gallery experience home, Urth Art prints are protected by art-grade acrylic glazing that prevents fading and discolouration.

T-mount

This page titled 3.3B: Phase-Contrast Microscopy is shared under a CC BY-SA 4.0 license and was authored, remixed, and/or curated by Boundless via source content that was edited to the style and standards of the LibreTexts platform.

In phase-contrast microscopy, parallel beams of light are passed through objects of different densities. The microscope contains special condensers that throw light “out of phase” causing it to pass through the object at different speeds. Internal details and organelles of live, unstained organisms (e.g. mitochondria, lysosomes, and the Golgi body) can be seen clearly with this microscope.

The easiest way to find and confirm your lens mount is to know the mount of the camera it was used on originally. A quick internet search of the camera model followed by lens mount is a sure way to confirm what lens mount you need. For example if you have an old Nikon lens and you know it was used on a Nikon FM2, you can easily find that the lens has a Nikon F-mount. This is particularly useful for lenses that are made for different mounts i.e Sigma and Tamron bring out the same lens with different mounts including EF, F, E so knowing the camera the lens was used on is a good shortcut.

Because you need to use your camera in full manual mode, you may need to familiarise yourself with this before shooting. You’ll soon work out which lenses you enjoy using with your camera. Analogue lenses can provide professional quality glass at a fraction of the cost of modern lenses so lens adapters can help you shoot with better lenses for less.