Numerical aperture formula

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Acceptance angle

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The numerical aperture of a lens is a measure of the ability of the lens to gather and focus light. It is defined as the sine of the maximum angle of light that can enter the lens and still be focused to a point on the image plane.

The numerical aperture of a lens is a physical property and cannot be changed. However, by using different techniques such as immersion microscopy or using different lenses with varying NA, the effective numerical aperture can be altered.

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A higher numerical aperture results in a shallower depth of field, meaning that only a small portion of the specimen will be in focus at a time. Conversely, a lower numerical aperture results in a deeper depth of field, allowing more of the specimen to be in focus at once.

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A higher numerical aperture generally results in better resolution, as a larger NA allows for a smaller focused spot size, resulting in finer detail in the image. However, other factors such as lens quality and lighting conditions also play a role in resolution.

What's the Difference Between an Ocular Lens and an Objective Lens? Most microscopes used in schools and labs have at least two, and usually more, lenses ...

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The numerical aperture of a lens is calculated by dividing the refractive index of the medium in which the lens is located by the distance from the lens to the focal point.

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Sep 18, 2023 — Focal length is important because it relates to the field of view of a lens – that is, how much of the scene you'll capture. It also explains ...