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Fluorescence imaging describes the field of imaging technologies designed to visualize the spatial distribution of substances that emit fluorescence. These techniques incorporate a light source of appropriate wavelength that is used to excite the fluorochromes and a detection system that can perform spectral and spatial separation to differentiate and spatially resolve fluorescence emitted from the fluorescence substances. In its broader sense fluorescence imaging for biomedical applications includes fluorescence microscopy techniques, macroscopic fluorescence imaging of biological specimens and assays, for example in fluorescent gels or plates, and other tissue imaging.

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Intes X, Ripoll J, Chen Y et al (2003) In vivo continuous‐wave optical breast imaging enhanced with indocyanine green. Med Phys 30:1039–1047

Fluorescence mediated tomography; Fluorescence molecular tomography, FMT; Fluorescence reflectance imaging, FRI; Fluorescence tomography; Fluorescence transillumination imaging

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Ntziachristos, V. (2008). Fluorescence Imaging. In: Baert, A.L. (eds) Encyclopedia of Diagnostic Imaging. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35280-8_967

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Ntziachristos V, Yodh AG, Schnall M et al (2000) Concurrent MRI and diffuse optical tomography of breast after indocyanine green enhancement. Proc Natl Acad Sci USA 97:2767–2772

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Ntziachristos V, Ripoll J, Wang LHV et al (2005) Looking and listening to light: the evolution of whole‐body photonic imaging. Nat Biotechnol 23:313–320

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