There are also often color-coded rings indicating different magnification values, e.g. black for 1 ×, yellow for 4 ×, green for 10 ×, etc.

Edmund Optics offers a wide variety of microscopy components including microscope objectives, inverted and stereo microscopes, or optical filters that are ideal for use in microscopy setups. Microscope objectives are available in a range of magnifications and include infinity corrected, finite conjugate, and reflective objectives in industry leading brands such as Mitutoyo or Olympus. Microscope objectives are ideal for a range of research, industrial, life science, or general lab applications. Microscopy filters are ideal for isolating specific wavelengths in fluorescence imaging applications.

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For such applications, chromatic aberrations are often no issue, so that one does not exploit the chromatic correction of the objective. Also, a wide field of view would not be required. On the other hand, a microscope objective for visible light may well not have ideal properties e.g. for launching near infrared light into a fiber, and its power handling capability is limited (but usually not specified). Therefore, a microscope objective may not be the ideal solution for such an application. However, it may have to be used, e.g. if no other lenses are available for reaching the required small spot size.

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In most cases, a microscope objective is mounted on the nosepiece of a microscope using a thread. Unfortunately, there are different thread sizes used by different manufacturers and for objectives of different kinds. In some cases, special adapters can be used for applying an objective to a microscope with different threads.

The first image shows the eyepiece view when using a 1.0X objective with a 10X eyepiece. It has a 34mm field of view. The second image shows the video field of view of about 16–4.7mm (COLCAM-NTSC camera with a 0.5X coupler). The third image shows the video view that approximates the eyepiece view. It uses a 0.5X objective with a 20X eyepiece.

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Particularly for objectives with high numerical aperture, a high image quality can be achieved only with substantial efforts for correcting various kinds of optical aberrations such as spherical, astigmatism, coma, field curvature, image distortion and chromatic aberrations. For example, plan-apochromatic objectives, having particularly sophisticated designs, provide optimum flat field correction combined with good achromatic properties.

What is the purpose of theobjectivelens in a lightmicroscope

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Note that oil immersion may not work properly e.g. when observing a biological sample in an aqueous solution and the oil is only between the cover slip and the objective. One may have to use special water immersion objectives for such cases.

Optical microscopes usually work based on imaging with visible light, i.e., in the wavelength region from 400 nm to 700 nm. Therefore, most microscope objectives are optimized for that wavelength range, with most emphasis on the region from 480 nm to 640 nm. However, there are objectives with an enhanced range of e.g. 400 nm to 950 nm, and others which work further in the infrared. For example, that is required for laser microscopes where infrared laser beams need to be transmitted.

Achromatic objectives–This objective brings red and blue light to a common focus, and is corrected for spherical aberrations for green. It is excellent for black and white viewing. If an objective is not labeled, it is achromatic.

The focal length of a lens is the optical distance (usually measured in mm) from the point where the light meets inside the lens to the camera's sensor.

Microscopes often contain multiple objectives on a rotatable nosepiece, for example a scanning lens with only 4 × magnification, an intermediate one (the small objective lens) with 10 × and a high-resolution large objective with 40 × or 100 × magnification. The eye piece may contribute another factor 5 or 10 in magnification, for example.

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Infinity Correction–When measuring from the back end of the objective to the primary focal plane, many microscopes are limited to a specific distance (160mm). More expensive microscope use a different series of lenses, prisms and mirrors to allow for an "infinite" distance between those two points. This is called infinity correction.

Older microscopes usually require finite-corrected objectives. Here, the object is supposed to be placed a little below the front focal plane of the objective, and the intermediate image occurs at a finite distance of e.g. 160 mm from the objective. Such an objective is designed for minimum image distortions in that configuration.

Objectives for dark-field illumination are tentatively larger, providing extra space for the illumination light; therefore, they are typically used with larger threads.

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What is the job of theobjectivelenses

The focal length of a microscope objective is typically between 2 mm and 40 mm. However, that parameter is often considered as less important, since magnification and numerical aperture are sufficient for quantifying the essential performance in a microscope.

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A microscope objective lens produces a real, magnified image of an object placed within its field of view. The image is then further magnified by the ocular ...

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Some microscopes allow the injection of illumination light through the objective to the sample. It is then important that there is no significant scattering of light in the objective.

Apochromatic objective–This is the most expensive objective. It is chromatically adjusted for four colors (deep blue, blue, green and red) and spherically corrected for deep blue, blue and sometimes green. This is the best choice for color viewing. These have a higher numerical aperture (N.A.) than achromats or fluorites.

Problem: The PZMIII or PZMIV stereo zoom microscope normally comes with a 1.0X objective and a 10X pair of eyepieces. The magnification is 6X to 50X, however the concept of magnification is difficult to visualize. Let's discuss what can be seen at the two zoom extremes. Imagine the visual circle to be a range of 34–4.2 mm. This microscope has a working distance of 100mm. Researchers working with small animals will have difficulty working in this tight space.

by F Vatansever · 2012 · Cited by 400 — Far infrared (FIR) radiation (λ = 3–100 μm) is a subdivision of the electromagnetic spectrum that has been investigated for biological effects.

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Solution: Instead of the standard configuration, setup the microscope with a 0.5X objective to increase the working distance to 187 mm. The result of using this lower power objective is that the magnification range decreases by one half and at the same time the field of view double. To restore the microscope system to the original condition (magnification and field of view), replace the 10X eyepieces with 20X eyepieces. The use of these two options restores the visual field of view and magnification range back to the original condition with the added benefit of a larger working distance.

There are also reflective objectives, containing curved mirrors and no lenses. They are naturally achromatic and may be advantageous for operation in extreme wavelength domains. Also, they can exhibit lower losses of optical power.

Objectivelens

Finite-corrected objectives are always designed for a certain tube length, e.g. according to DIN or JIS standard (which differ by 10 mm in tube length). Using an objective of the wrong standard may significantly deteriorate the obtained image quality.

A variety of microscope objectives are available. All objectives use lenses to focus light. Light is broken down into various wavelengths (colors) as it travels through a lens. The various wavelengths have different focal points. That means that red, green and blue appears to focus at different points. This is called chromatic aberration. Spherical aberrations are focal mismatches caused by the shape of the lens. Quality lenses are designed correct for chromatic and spherical aberration to bring the primary colors to a common focal point. These terms may help you determine the best objective for your application:

Microscope objectives are sometimes used for applications outside microscopy. For example, they can be used for tight focusing of laser beams, with spot sizes of a few micrometers or even below 1 μm. If the input beam is a collimated beam, an infinity-corrected objective will work best. The objective should have a numerical aperture which fits well to the beam divergence related to the required spot size. The input beam radius should also be chosen appropriately, i.e., calculated from the required spot size and the focal length. A difficulty may be to find out the focal length, as the objective barrel often only indicates the magnification, and the conversion to the focal length depends on the microscope design.

Lumenmicroscope

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Fluorite or semi-apochromat objectives–These lenses are chromatically corrected for red and blue, and the green focus is also close. They are spherically corrected for blue and green. This objective is better suited for color viewing or recording than achromatic objectives.

Most microscopes objectives are based on refractive optics, containing several lenses. For example, a simple low-NA objective may contain a meniscus lens and an achromat. A high-NA objective typically contains a more complicated combination of various types of lenses of hemispherical, meniscus, achromatic doublet and triplet type.

Note that a large magnification alone is not helpful if it only makes images larger without increasing the level of detail; see below the section on the numerical aperture.

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Unfortunately, perfect solutions are not available; therefore, one has to accept certain trade-offs, which lead to different optimized solutions for different applications. For example, optimum flat field properties are most important for measurement microscopes; one may then tolerate somewhat larger chromatic aberrations.

The objective lens is the most important part of a microscope and plays a central role in imaging an object onto the human eye or an image sensor for discerning the object’s detail. Microscope objectives are ideal for a range of science research, industrial, and general lab applications.

Although a microscope objective is sometimes called the objective lens, it usually contains multiple lenses. The higher the numerical aperture and the higher the required image quality, the more sophisticated designs are needed. High-end microscope objectives may also involve aspheric lenses.

The higher the magnification, the higher is also the required numerical aperture because this is the factor which ultimately limits the achievable image resolution. There are different ways of calculating the image resolution and are slightly different circumstances, but they lead to similar resolution values, which are roughly <$\lambda / (2 NA)$>, where <$\lambda$> is the optical wavelength (about 400 to 700 nm) and NA is the numerical aperture. For example, an NA of 1 allows for an image resolution of roughly 250 nm for green light. For low magnification, an NA of 0.1 may be fully sufficient.

The microscope objective is a key component for reaching high performance of a microscope. It is the part which is placed next to the observed object, usually in a fairly small distance of a few millimeters. Usually, the microscope objective produces an intermediate image in the microscope, which is then further magnified with an eyepiece (ocular lens). Particularly in cases with high magnification, most of the magnification is provided by the objective.

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Objectivelensmicroscope

At least for high magnifications, the influence of a cover slip in terms of chromatic and spherical aberrations can be quite important. Therefore, objectives for use in fields like biology, where cover slips are often needed, are designed with integrated cover slip correction. The correction is often done for a standard slip thickness of 170 μm. A deviation of only 10 μm can already be quite problematic for an objective with a high NA of e.g. 0.95. Some objectives allow the adjustment of the corrected cover slip thickness.

The design of a high quality microscope objective is a rather sophisticated task, for which substantial optics expertise and powerful optics design software are required. Such designs involve complicated trade-offs, which should be properly handled according to the importance of different aspects for a particular application.

What does theobjectivelens doonamicroscope

Shanghai Optics custom microscope objectives are designed with the assistance of CAD, Solidworks and Zemax software using top quality glass having highly specific refractive indices. This enables us to produce microscope objectives that are very low in dispersion and corrected for the most of the common optical artifacts such as coma, astigmatism, geometrical distortion, field curvature, spherical and chromatic aberration.

TIP: On the trinocular version of the PZMIII or PZMIV stereo microscope with the standard configuration (1.0X objective, 10X eyepieces) and with the optimal camera adaptor (0.5X on a ½” CCD camera) the video capture field of view is up to 40% less than the visual field. By using a 0.5X objective with 20X eyepieces the video capture area doubles, and the resulting video capture more closely matches the visual field of view.

Another application is launching light into a single-mode fiber or collimating light from such a fiber. Again, the objective should have an appropriate numerical aperture of the order of that of the fiber. For more details, see the article on fiber launch systems.

Note that some microscope designs count on the correction of some residual aberrations of the objective by the ocular lens.

Plan objective–These objectives produces a flat image across the field of view. The three objectives discussed above all produce a curved image. A plan-achromat, plan-fluorite or plan-apochromat are corrected.

Modern microscopes mostly require infinity-corrected objectives, where the intermediate image of the objective alone lies at infinite distance. Here, one requires an additional tube lens in the microscope for generating the intermediate image at the diaphragm of the eyepiece.

NOTE: If a 1/3” inch camera (6mm diagonal) is used on the 0.5X microscope adaptor you can apply the ratio of 6/8 for the reduction in the captured field.

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Chromatic aberrations essentially result from the wavelength dependence of focal length. They lead to colored image distortions. For conventional microscopy, they can be quite relevant, in contrast to other types of optical microscopy, e.g. certain types of laser microscopy. Best suppression of chromatic aberrations is achieved with apochromatic objectives.

Note that it is essential not only to have a good transmittance over the full wavelength range, but also achromatic performance. In conventional light microscopes, this is needed to avoid colored image distortions. In confocal multi-photon fluorescence microscopes, it is important to have the same focus positions for infrared laser light as for the fluorescence light.

The magnification of the image depends on the combination of the eyepiece and the objective used. This combination also affects the field of view. This example shows how these factors inter-relate.

Another practically important factor is the working distance, i.e., the distance between the objective and the object. Small working distances are generally required for objectives with high NA, but also can to some extent be optimized as a design goal (possibly somewhat compromising the NA or the correction). For objectives with oil immersion, a relatively small working distance is actually good, since otherwise one would require more of the immersion fluid, and that would be more difficult to hold in place.

The highest numerical apertures achievable with dry objectives, operated with air between the objective and the object, are approximately 0.95. Substantially higher values of e.g. 1.5 or even higher can be achieved with immersion objectives, where the gap between the object and the objective is filled with a liquid – water or some immersion oil with a higher refractive index, often somewhat above 1.5. Optimized immersion oils do not only have a high refractive index, but also a suitable viscosity and a low tendency for producing stains on the surfaces. They can be left on an objective over longer times without damaging it.