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Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

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A job in illumination engineering involves the deliberate application of light to achieve some aesthetic or practical effect. Light sources include artificial light from lamps as well as daylight from windows and skylights. Artificial lighting represents a major component of energy consumption, accounting for a significant part of all energy consumed worldwide. A career in photonics means working on the generation, emission, transmission, modulation, signal processing, switching, amplification, detection and sensing of light. Because there are so many job opportunities in photonics, it can be considered a separate career path.

1Fluorochrome excitation and emission data were generated on a spectrophotometer and spectrofluorometer, respectively. Data for immunofluorescence fluorochromes were collected using conjugated antibodies.

3Fluorochrome spectral signatures were generated on the respective instrument platform using recommended set-up protocols. Spectral signatures are dependent on the cytometer configuration and settings used, and, as a result, results on individual instruments may differ from what is depicted herein.

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2Collection of pre-loaded configurations includes the following flow cytometers: BD Accuri™, BD FACSAria™ Fusion, BD FACSAria™ III, BD FACSCalibur™, BD FACSCelesta™, BD FACSJazz™, BD FACSLyric™, BD FACSMelody™, BD FACSVerse™, BD FACSymphony™ A1, BD FACSymphony™ A3, BD FACSymphony™ A5, BD FACSymphony™ A5 SE, BD FACSymphony™ S6, BD® LSR II, BD LSRFortessa™, BD LSRFortessa™ X-20.

For the purpose of recruiting and looking for a career, optics and lasers go together because of the size of the market for lasers. In 2004, approximately 131,000 lasers and 733 million diode lasers were sold with a value of over $5 billion. Since their invention in 1960, they have become ubiquitous with supermarket barcode scanners and laserdisc players. There are jobs with lasers in medicine (cosmetic and eye surgery), industry (cutting and welding), defense (marking targets and defending against missiles), and scientific research (spectroscopy and interferometry).

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The BD® Spectrum Viewer is an interactive tool that supports optimized fluorochrome selection, choosing filters and assessing potential spillover when designing flow cytometry panels. The latest and improved version of the tool offers several additional features: ability to view and compare excitation (Ex) and emission (Em) spectra with a broad and growing library of dyes and probes;1 an extensive collection of pre-loaded cytometer configurations;2 a stacked laser view and the ability to save and share your work; and the ability to visualize and analyze fluorochrome emission profiles on spectral flow cytometers3 with a new heat map option to more easily assess areas of overlapping emission. Explore the new BD® Spectrum Viewer below or access the legacy BD® Spectrum Viewer.

Use BD RC to go beyond the fluorochrome spectra and into panel design. The predefined Reagent Selector workflow guides you through the panel design process and provides a customized experience by filtering reagent choices according to your instrument configuration and reagent availability so that you only build a panel that is compatible with your instrument and available for ordering. Create your own custom panels or use one of our pre-optimized multicolor flow cytometry panels. Share the panel with collaborators and save time on the cytometer by translating your panel into a BD FACSDiva™ Software experiment directly in BD RC. Sign up for a free account, configure your cytometer and start designing your panel today at bdresearchcloud.com

A career in optical physics or engineering means knowing about the behavior and properties of light, including its interactions with matter and its detection by instruments. Since the discovery that light is electromagnetic radiation, optics has largely been regarded in theoretical physics as a subfield of electromagnetism. Some optical phenomena depend on the quantum nature of light and the interaction of light with matter. Geometric optics and physical optics are models that describe phenomena while ignoring properties of light that are insignificant for the system of interest. A job in optical science might require a background in electrical engineering, psychology, and medicine (particularly ophthalmology and optometry).

Careers in optoelectronics require an understanding of the quantum mechanical effects of light on semiconducting materials. Optoelectronics is the study and application of electronic devices that produce, detect, and control light. In this context, light often includes invisible forms of radiation such as gamma rays, X-rays, ultraviolet and infrared, in addition to visible light. Optoelectronic devices are electrical-to-optical or optical-to-electrical transducers, or instruments that use such devices in their operation. There are jobs in the design and manufacture of devices that use the photoelectric or photovoltaic effect. Such devices are photodiodes (including solar cells), phototransistors, photomultipliers, and elements for integrated optical circuits (IOC). The phenomena of photoconductivity is used in photo resistors and charge-coupled imaging devices. The phenomenon that is the basis for lasers is stimulated emission.