Long Working Distance Microscope Objectives are designed around 426 to 656 nm to provide a chromatic aberration-free image with flat field correction. The objectives are called “Plan” because they produce the flat image across the field of view. The “apochromat” objectives provide chromatic correction for three wavelengths and spherical correction for two wavelengths. In the case of white light, plan apochromatic objectives offer superior images for color photomicrography than achromatic objectives can provide.

In general, the focal length and working distance of objective lens varies very little with wavelength and is usually not specified at a particular wavelength. At most, over a specified wavelength range, one could potentially see only microns of deviation, and this amount would be even smaller for apochromatic objectives.

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Aperture

Simply take the Focal Length of the lens and divide it by the Diameter of the Entrance Pupil (maximum aperture opening) and viola! You have the F-Ratio. The example below shows this calculation on a Canon EF 50mm f/1.8 II.

Many times microscope objectives with high magnifications will have very short working distances to the focused spot. Because of this short distance, if one is not careful while focusing in on the targeted specimen, the end of the microscope objective may "crash" into the object under study. These Long Working Distance Objectives are ideal to use when the application requires there be room between the objective and the target for other equipment like pipettes or syringes. These objectives have some of the world's longest working distances.

F-stops

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Long Working Distance Microscope Objectives are designed around 426 to 656 nm to provide a chromatic aberration-free image with flat field correction. The objectives are called “Plan” because they produce the flat image across the field of view. The “apochromat” objectives provide chromatic correction for three wavelengths and spherical correction for two wavelengths. In the case of white light, plan apochromatic objectives offer superior images for color photomicrography than achromatic objectives can provide. In general, the focal length and working distance of objective lens varies very little with wavelength and is usually not specified at a particular wavelength. At most, over a specified wavelength range, one could potentially see only microns of deviation, and this amount would be even smaller for apochromatic objectives.

Focal length

The thread on the long working distance objectives are M26, so the M26-RMS adapter is required to step down to standard RMS threads.  Please make sure to purchase one for use with any RMS mount such as the LH-OBJ microscope objective holder.

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Fstops explained

Many times microscope objectives with high magnifications will have very short working distances to the focused spot. Because of this short distance, if one is not careful while focusing in on the targeted specimen, the end of the microscope objective may "crash" into the object under study. These Long Working Distance Objectives are ideal to use when the application requires there be room between the objective and the target for other equipment like pipettes or syringes. These objectives have some of the world's longest working distances.

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In addition, the lens with an aperture of f/2.8 will provide for a stronger out-of-focus effect in the background objects behind the subject in focus as shown below.

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Numerical aperture

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f-stop是什么

Many of you will already know what the F-Ratio means in the real world. From an in practice standpoint, the F-Ratio is simply telling you the maximum amount of light that a lens can allow in, as well as the potential rack-focus strength or bokeh in objects that are out of focus.

A T-stop is the measure of light that actually arrives at the sensor. Why is there a difference? Light doesn’t cleanly arrive at the sensor as some of it is reflected, and absorbed by part of the lens etc, and the T-stop accounts for this. So a T-Stop is, in effect, a reflection of the real speed of the lens.

Shutter speed

So while, for example, a lens’ aperture may be open to 1.4, the actual measure of light hitting the sensor may, in fact, be equivalent to 1.7. That actual number, 1.7, is the T-Stop, and some lenses, typically cinema lenses, will be rated as such.

Infinity corrected objectives are used in a wide variety of imaging and laser focusing applications. Light rays leaving the objective’s rear aperture are collimated, so that for imaging applications, a secondary lens (usually called a tube lens) is needed in order to focus the collected light from the specimen onto the sensor. The labeled magnification is calculated, assuming the objective is being used with a tube lens of a particular focal length by design. When a tube lens of a different focal length is used, the magnification will need to be adjusted accordingly. As an advantage over finite conjugate objective lenses, a variety of auxiliary optical components, such as optical filters and polarizers can be inserted between the infinity objective lens and the tube lens without altering how the beam propagates and forms the image down the optical path. In laser applications such as optical tweezers and laser cutting, laser beams entering the rear aperture of an infinity objective can be tightly focused to a diffraction-limited spot, providing concentrated optical power and excellent resolution.

Infinity corrected objectives are used in a wide variety of imaging and laser focusing applications. Light rays leaving the objective’s rear aperture are collimated, so that for imaging applications, a secondary lens (usually called a tube lens) is needed in order to focus the collected light from the specimen onto the sensor. The labeled magnification is calculated, assuming the objective is being used with a tube lens of a particular focal length by design. When a tube lens of a different focal length is used, the magnification will need to be adjusted accordingly. As an advantage over finite conjugate objective lenses, a variety of auxiliary optical components, such as optical filters and polarizers can be inserted between the infinity objective lens and the tube lens without altering how the beam propagates and forms the image down the optical path. In laser applications such as optical tweezers and laser cutting, laser beams entering the rear aperture of an infinity objective can be tightly focused to a diffraction-limited spot, providing concentrated optical power and excellent resolution.

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f-stop app

These objective lenses are an M Plan Apochromatic design which offers a flat field correction, long working distances and superior optical performance. These objective lenses offer some of the world’s longest working distances while providing a flat, chromatic aberration-free image throughout the entire field of view.

The thread on the long working distance objectives are M26, so the M26-RMS adapter is required to step down to standard RMS threads.  Please make sure to purchase one for use with any RMS mount such as the LH-OBJ microscope objective holder.

For example, a lens with a maximum aperture of f/2.8 will allow double the amount of light as a lens with a maximum aperture of f/4.0 as shown below.

While an F Number may suggest how much light may pass through the lens, it’s not entirely an accurate measure of how much really gets all the way through to the sensor due to light absorbance and reflection etc..

In this article, we are going to talk about the F-Ratio. What’s the F-Ratio you ask? Well, the F-Ratio is that little f-number written on your lens next to the focal length. For example, on Canon EF 50mm f/1.4 the f/1.4 number is the F-Ratio. But what exactly is the F-Ratio and how is it determined?

It’s important to understand however, that if you have a lens set to f/1.4 and it has a T value of 1.6, the T value has no bearing on depth of field. The fact that the F-stop is based on a physical measurement means it is constant.