Cy5spectrum

A culture of male pig kidney cells (Sus scrofa; LLC-PK1 line) was immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5. Note the bright fluorescence intensity exhibited by the intermediate filament network, which extends throughout the cytoplasm in these epithelial cells.

Now this is more like it. A sinister outline of a child. This must be a ghost, surely, just like this one in the Mirror, which turned out definitely to be a ghost. Tim Sandys takes up the story.

“There is no light source in this cave. After examining the picture afterwards I surprised what showed up. First I thought something was wrong with my camera, but pictures before the snapshots and after the snapshots where fine. The other picture shows streams of misty light descending from the roof of the cave and bouncing off the cave floor.”

Canine kidney epithelial (Madin-Darby; MDCK line) cells were stained with phalloidin conjugated to Alexa Fluor 633, which targets the intracellular cytoskeletal F-actin network. Phalloidins are a member of the phallotoxin group of bicyclic peptides isolated from the deadly Amanita phalloides mushroom.

Anna Scordato and Stanley Schwartz - Bioscience Department, Nikon Instruments, Inc., 1300 Walt Whitman Road, Melville, New York, 11747.

Texas Redexcitation emission

“Taken at Chistlehurst caves in Kent whilst with my two young daughters,” says this anonymous reader. “Noticed the image when I uploaded it to my laptop.”

“Being a suitably dark and stormy night, the lights were occasionally flickering. The lighting system is a shambles, working intermittently and highly prone to problems thanks to its unpredictable motion-activated sensors. The lights were doing odd things. Clicking and burring - fluorescent tubes were sputtering and clacking.”

This filter combination is optimized for use with popular fluorescent probes such as Cy5, Alexa Fluor 647, and allophycocyanin (APC), when the dyes are used alone or in multiple labeling techniques, and is also recommended for applications with other fluorophores excited by orange to red wavelengths. The bandpass emission filter functions to reduce or eliminate interference from emission in the infrared spectral region beyond approximately 740 nanometers. The Cy5 HYQ filter set is recommended when studying the following fluorophores: Alexa Fluor 633 and 647, APC, BODIPY 650/665, Cy5, Cy5.1.8, DiD (indodicarbocyanine), DiIC1(5), DiSC3(5), MitoTracker Deep Red 633, NeuroTrace 640/660, Nile Blue, SYTO (derivatives 60, 62, and 63), TO-PRO-3, and TOTO-3. The images presented in Figure 2 demonstrate the performance of this filter combination with a variety of orange and red-absorbing fluorescence probes targeted at different intracellular locations.

Observe the fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells that were immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647. Note the bright fluorescence intensity exhibited by the intermediate filament network, which extends throughout the cytoplasm in these fibroblast cells.

“The quality of the image, the history of the building and the fact that it creeps most people out late at night, all these pandered to the arcane narrative; the familiar trope of a ghost story. I suspect someone was working up on the second floor in Printmaking. They likely had their bored child along for the ride who decided to go exploring downstairs and was greeted to the site of some creepy guy with a phone.”

The modulation transfer function (MTF) is a parameter for assessing the performance of a lens.

Guardian readers have been sending in their decidedly unconvincing pictures of ghosts. We investigate the ten most puzzling images and decide once and for all whether ghosts exist

“A possible otherworldly origin is seemingly suggested by the fact that this patch of South East London is especially renowned for its spooky goings on. The photo is taken outside one of a trio of drinking establishments known locally as “the New Cross Triangle.”

We were inundated with spooky responses from around the world. Were any of them actually of ghosts? Let’s run through them, as one can with ghosts, and see.

Cy3excitation emission

The USB 3.0 interface ensures swift data transmission, enabling real-time imaging and enhancing the efficiency of vision systems. USB 3.0 Cameras are ideal for ...

Swiss albino mouse embryo cells (3T3 line) stained with the carbocyanine dye DiD, which targets lipophilic organelles, including the plasma membrane, are presented in Figure 2(d). The visible light absorption maximum of DiD is 644 nanometers and the emission maximum occurs at 665 nanometers. Carbocyanine probes are often utilized in apoptosis experiments and as tracers for cellular migration.

Penises are really hard to describe. They're hard, so steel works. Nobody expects actual steel and steel is used to describe other hard things, ...

The Cy5 HYQ filter combination is designed with steep passband transitions, allowing the benefits of wide bandpass regions while maintaining sufficient signal separation to avoid an increased noise level relative to the detected emission. Such sharp filter transitions are created by increasing the number of interference cavities in both the excitation and emission filters, enabling a closer spectral proximity between the two bandpass regions. The resulting wider and steeper filter passbands allow the set to provide more excitation energy and capture a higher level of fluorescence signal. Because the range of fluorescence wavelengths transmitted by the Cy5 HYQ combination extends into the near-infrared spectral region, which is either invisible or only faintly visible to the human eye, detectors with near-infrared sensitivity must be used to maximize the captured fluorophore emission.

Our first ghost comes all the way from Australia, where hauntings by spooky apparitions are given an extra fright factor by being upside down.

Cy5absorbance

Compare the fluorescence emission intensity from the culture of rat kangaroo kidney cellular cytokeratin labeled with Cy5 to those listed above labeled with Alexa Fluor 647. The two fluorescent probes (Alexa Fluor 647 and Cy5) have similar absorption and emission spectral profiles and produce comparable results with the Nikon Cy5 HYQ filter combination.

Examine the fluorescence emission intensity from a culture of rat kangaroo kidney epithelial cells (PtK2 line) that were immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647.

The polarizing effect is particularly strong when sunlight is scattered through 90° (Fig. 14-13). On a clear day, when there is little water vapor or dust in ...

Cy5vs Alexa 647

Center thickness is measured across the mechanical axis of the lens, defined as the axis exactly between its outer edges. ... mirror, window, prism, or plano-lens ...

Fluorescence emission in a culture of rat kangaroo (PtK2 line) epithelial kidney cells that were immunofluorescently labeled with primary anti-vimentin mouse monoclonal antibodies followed by goat anti-mouse F(ab')2 fragments conjugated to biotin is demonstrated in Figure 2(c). The biotin secondary antibody was subsequently absorbed with a streptavidin conjugate of QDot 655. The visible light absorption extinction coefficient of QDot 655 occurs over a wide range that increases with decreasing wavelength. However, unlike the case with organic chromophores, fluorescence emission features a symmetrical profile in quantum dots (centered at 655 nanometers for this species).

Cy5Structure

Examine a culture of bovine pulmonary artery endothelial cells stained with MitoTracker Deep Red 633, which targets the intracellular mitochondrial network. The visible light absorption maximum of MitoTracker Deep Red 633 is 644 nanometers and the emission maximum occurs at 665 nanometers.

An adherent culture of rat kangaroo kidney epithelial cells (PtK2 line) was immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5. Note the bright fluorescence intensity exhibited by the fibrous vimentin intermediate filament network, which extends throughout the cytoplasm in these epithelial cells.

by RE Parks · 1973 · Cited by 4 — Recently Frank Cooke, Inc., was asked to make three aspheric lens elements, the aspheric surfaces being described by spline functions.

“I was pottering in my studio which is located in the basement level of my building. Now part of the postgraduate art school here in Glasgow, it once was a hospital as is very apparent from its slightly imposing medical trappings.”

The cells (3T3 line) were immunofluorescently labeled with primary anti-human golgin-97 mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5. Anti-human golgin-97 antibodies recognize a 97-kiloDalton protein termed golgin-97, which is a member of the granin protein family and a peripheral membrane protein localized on the cytoplasmic face of the Golgi apparatus.

“This poltergeist appeared behind us during this photograph taken at the South East Asia games in Singapore at the National Stadium in Kallang, says Jason Elliott. It’s not a poltergeist.

Essentially, visible light passes through glass, but glass acts like a mirror for infrared wavelengths (which is why thermal imaging camera lenses are often ...

“In 2011 I went to a party with a group of nursing students. We visited the old Manly Quarantine Station and did a ghost tour. I don’t believe in ghosts but I did enjoy hearing all the macabre stories from yesteryear. One recurring story was of visitors being followed at night by disembodied blazing red eyes. I got bit of a surprise when I checked my photos the next day.”

“On our way to the top of Blarney Castle, I snapped two pictures of a dark cave carved into the side of the castle which was used to house prisoners,” says Alexandra Bacchetti

Cathode Ray Tube (CRT):. CRT stands for Cathode Ray Tube. CRT is a technology used in traditional computer monitors and televisions. The image on CRT display is ...

DaLeftHook left nothing to chance, and dared friends (note: not himself) to go to Long Beach Cemetary at midnight. These brave, unnamed friends took this picture, apparently of a gravestone. They were never seen again.

Used in conjunction with the BJU Press Chemistry Lab Manual, The Logos Science Chemistry Addendum and Lab Kit contains information, equipment, and supplies ...

Cy5color

“Spooky apparition seen in Rochester cemetery, Medway,” says Matt McMillan, who has optimistically captioned his photo ‘Ghost of a Squirrel’.

Fluorescence emission intensity from a culture of rat kangaroo (PtK2 line) epithelial kidney cells that were immunofluorescently labeled with primary anti-vimentin mouse monoclonal antibodies followed by goat anti-mouse F(ab')2 fragments conjugated to biotin.

Autofluorescence emission intensity from starch grains and other components in a thin section of potato (Solanum tuberosum) tuber tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll.

“Indeed, it has been proposed that a pale, squinting gentleman regularly spotted nearby, sporting a pointy beard and silken breeches, might not be a local art student as first thought, but rather could be none other than the phantom of murdered Tudor playwright Christopher Marlowe himself!”

Ultraviolet, visible, and near-infrared transmission spectral profiles for components of the high-performance Nikon Cy5 HYQ filter combination are illustrated below in Figure 1. Each individual component in this filter set is specifically designed to optimize detection of fluorescence from the fluorochrome Cy5 (along with spectrally similar probes), and the set also provides excellent performance for the majority of fluorophores excited by red wavelengths. The filter combination utilizes wide passband excitation and emission filters to provide maximum signal level, as is characteristic of the Nikon HYQ filter combination group.

“I decided to make a slow-motion capture of the corridor lights flicking on and off. I shot several clips over a twenty minute period. As I reviewed the footage, I became aware that some shots had the figure of a little girl standing at the end of the corridor, seemingly looking at me from around the door.”

Inspired by an early silly season spate of spooky ghost stories in the press, we asked our readers to provide their own ghost pictures. We asked this despite knowing full well that ghosts aren’t real, as comprehensively proved by the BBC’s Ghostwatch investigation in 1992.

Presented in Figure 2(a) is the fluorescence emission profile from a culture of Indian Muntjac deerskin fibroblast cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5. The visible light absorption maximum of Cy5 is 649 nanometers and the emission maximum occurs at 670 nanometers. All of the images in Figure 2 were captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the microtubule network in Figure 2(a).

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy5.

Fluorescence emission intensity from a culture of HeLa cells that were immunofluorescently labeled with primary anti-histone (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647.

Fluorescence emission intensity from a culture of male pig kidney epithelial cells (Sus scrofa; LLC-PK1 line) stained with MitoTracker Deep Red 633, which targets the intracellular mitochondrial network.

Autofluorescence emission intensity from starch grains and other components in a thin section of potato (Solanum tuberosum) tuber tissue is demonstrated in Figure 2(f). Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the red absorption region, chlorophyll has an absorption band with a low extinction coefficient, but still produces a detectable level of fluorescence at emission wavelengths of 650 nanometers and above (deep red region). For the starch grains illustrated above, note the presence of autofluorescence emission intensity in the red spectral region.

Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins.

“If you look closely in this picture, you can make out a ghostly figure standing in the middle-left window of the pub, just above the sign,” claims endymionsleeps.

Figure 2(e) illustrates fluorescence emission in a culture of HeLa cells that were immunofluorescently labeled with primary anti-histone (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647. The visible light absorption maximum of Alexa Fluor 647 is 649 nanometers and the emission maximum occurs at 666 nanometers (in the deep red region of the visible light spectrum). The primary antibody, produced from purified nuclear fractions of HeLa cells, binds specifically to an antigen determinant that is present on all five histone proteins (H1, H2A, H2B, H3, and H4).

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“Supposedly the nickname stems from the fact that many people have been known to enter “the Triangle” for a swift half, only to disappear for days on end, emerging confused and with little memory of what has transpired. The locality has been noted for its frequent ghost sightings.”

A culture of bovine pulmonary artery endothelial cells was stained with phalloidin conjugated to Alexa Fluor 633, which targets the intracellular cytoskeletal F-actin network. The visible light absorption maximum of Alexa Fluor 633 is 621 nanometers and the emission maximum occurs at 639 nanometers.

Cy7excitation emission

You have to hand it to this ghost. Unlike the other ghosts on offer, it looks like a ghost. But it loses points for being seen during the daytime, as no self-respecting ghost ever would. Also, it’s joined this tourist snap a little too readily to convincingly be a tormented soul, trapped between this world and the next. And yet. And yet...

A culture of male pig kidney epithelial cells (Sus scrofa; LLC-PK1 line) stained with MitoTracker Deep Red 633 is presented in Figure 2(b). The visible light absorption maximum of MitoTracker Deep Red 633 is 644 nanometers and the emission maximum occurs at 665 nanometers. Fluorescence emission intensity exhibited by the tubular mitochondrial network extends throughout the cytoplasm of the central cell in the image.

The Cy5 HYQ set employs a bandpass emission (barrier) filter (pass band region between 663 and 738 nanometers) instead of a longpass variety, in order to limit interference from fluorophores emitting outside the intended wavelength region, and to reduce autofluorescence. The 75-nanometer emission window is combined with a wide 60-nanometer excitation passband (590 to 650 nanometers) to allow selective excitation and detection of specific popular fluorophores, which have absorption peaks in the orange to red spectral region. A longpass dichromatic mirror (660-nanometer cut-on wavelength) is provided in the Cy5 HYQ filter combination.

Observe the fluorescence emission intensity from a culture of canine kidney epithelial (Madin-Darby; MDCK line) cells stained with the carbocyanine dye DiD, which targets lipophilic organelles, including the plasma membrane. Carbocyanine probes are often utilized in apoptosis experiments and as tracers for cellular migration.

John D. Griffin, Nathan S. Claxton, Matthew J. Parry-Hill, Thomas J. Fellers, Kimberly M. Vogt, Ian D. Johnson, Shannon H. Neaves, Omar Alvarado, Lionel Parsons, Jr., Michael A. Sodders, Richard L. Ludlow, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

Fluorescence emission intensity from a culture of albino Swiss mouse embryo cells (3T3line) stained with the carbocyanine dye DiD, which targets lipophilic organelles, including the plasma membrane.