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Objective lensmagnification
Leica apochromats are objectives for applications with highest specifications in the visual range and beyond, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 1.0 x depth of field of the objective.
The optics of the most basic microscope includes an objective lens and ocular or eyepiece. The objective lens is closest to the sample, specimen, or object being observed with the microscope (see the schematic diagram below). For more information, refer to the article: Optical Microscopes – Some Basics Show schematic diagram
Whatarethe3objectivelenses on amicroscope
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Leica achromats are powerful objectives for standard applications in the visual spectral range, offering field flatness (OFN) up to 25 mm. The absolute value of the focus differences between red wavelength and blue wavelength (2 colors) is ≤ 2x depth of field of the objective.
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by J Sasián · 2021 · Cited by 10 — The distance between the objective lens and the thin wedge can be used to fine tune the correction because linear astigmatism depends on the marginal ray height ...
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Typesof objectivelenses
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Objective lensfunction
All Leica objectives are marked with codes and labels. These identify the objective, its most important optical performance properties, and the main applications it can be used for. For more information, refer to: Labeling of Objectives
To make it easier for you to find which Leica objectives work best for your microscope and application, you can take advantage of the Objective Finder
For standard applications, Leica Microsystems offers an extensive range of top-class microscope objectives. There are also Leica objectives which have been optimized for special applications. The highest-performance Leica objectives feature maximum correction and optical efficiency and have won several awards. All over the world, scientists are relying on Leica microscope objectives to gain insights into their area of research.
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What is objective lens in microscope
Leica microscope objective lenses are designed and made by our optics specialists to have the highest performance with a minimum of aberrations. The objectives help to deliver superior microscope image quality for many applications, such as life science and materials research, industrial quality control and failure analysis, and medical and surgical imaging.
Leica semi-apochromats are objectives for applications in the visual spectral range with higher specifications, offering field flatness up to 25 mm. The absolute values of the focus differences for the red wavelength and the blue wavelength to green wavelength (3 colors) are ≤ 2.5x depth of field of the objective.
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Stagemicroscopefunction
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Raman spectroscopy is based on scattering of incident light at an energy shifted by the vibrational energy (hν) of the molecule. Vibration modes for the same ...
Do you need an individual objective for your application? Then contact our Leica OEM Optic Center so that we can offer you a customized solution.
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The objective lens of a microscope forms a magnified, real, intermediate image of the sample or specimen which is then magnified further by the eyepieces or oculars and observed by the user as a virtual image. When a camera is used to observe the sample, then a phototube lens is installed after the objective in addition to, or even in place of, the eyepieces. The phototube lens forms a real image of the sample onto the camera sensor. The objective’s numerical aperture (NA), its ability to gather light, largely determines the microscope’s resolution or resolving power to distinguish fine details of the sample. Also, the working distance, the distance between the sample and objective, and the depth of field, the depth of the space in the field of view within which the sample can be moved without noticeable loss of image sharpness, both greatly depend on the properties of the objective lens. For more information, refer to: Collecting Light: The Importance of Numerical Aperture in Microscopy, How Sharp Images Are Formed, & Optical Microscopes – Some Basics & Labeling of Objectives
Field of view (FOV) is the extent of the observable, virtual or augmented world that is visible through a headset or device at a given moment.