Professor Charles Adams works in the Department of Physics at the University of Durham, is a Member of the Centre for Atomic and Molecular Physics and is Director of the Joint Quantum Centre.

Custom, low loss optics in the laser ring resonator enable output power levels >5 W. There is no compromise from having a single device covering 300 nm, and exceptional power levels are found at the edges of the tuning range. High powers of >2.5 W are possible in SHG.

M Squared’s SolsTiS laser platform transformed our quantum optics experiments, helping to produce several documented breakthroughs. In fact, we were so impressed with its performance and versatility that we went on to purchase another four.

Confocalmicroscopy protocol

Located in room D28 of the Lyman Duff Medical Building, the Department of Microbiology and Immunology is equipped with a Fluoview 1000 laser-scanning confocal microscope and associated computing platform from Olympus. The FV1000 microscope in our department contains 458/488/515/543/633 nm wavelength lasers allowing for simultaneous multi-colour imaging, 10-100x objectives, live imaging capability and has been recently upgraded (2014-2015) with high resolution gadolinium detectors, a mechanical stage and a new computer equipped with stitching software for whole organ image capture. In addition, the microscope has been recently been equipped with an LED light source for epifluorescent microscopy to meet a range of experimental needs.

Fully automated with wavelength tuning and locking via a web interface or published set of TCP/IP controls. Option to use third-party applications such as LabVIEW and MATLAB. Resonator elements are also accessible through external voltage inputs for active feedback.

Confocalmicroscopy applications

This SolsTiS extension adds a frequency quadrupling feature to SolsTiS, producing a narrow linewidth, tunable output in the ultraviolet wavelengths.

Individuals interested in using our facility must receive on-site training with our imaging consultants MIA CELLAVIE Inc. prior to gaining room access and microscope use. Training sessions will take 6-8 hours and are $560/person (one additional person may be trained per session for an additional $130). This one time non-refundable fee, paid up front, will be recouped by the user in the form of 25 non-consecutive hours of free use.

Professor Miles Padgett holds the position of Professor and Kelvin Chair of Natural Philosophy at the University of Glasgow’s School of Physics and Astronomy.

Confocalmicroscopy principle

Smallest laser in its class. A sealed resonator eliminates dust contamination and enables robust, reliable performance. Materials minimise effects from vibrations and thermal variations, resulting in a stable, low-frequency drift laser. Anti-humidity system and purge ports allow trouble-free operation across atmospheric absorptions.

Jan 27, 2017 — ... optical images to electronic signals. It ... Richie Merkz. 57 views•2 slides. This document ... A lens is a transparent single piece to focuses your ...

Ultra-narrow linewidths from <50 kHz absolute linewidth, with options to achieve Hertz level linewidth via an external, ultra stable reference. Overall, SolsTiS is the quietest and most stable TiS laser available with free running linewidths close to 50 kHz.

Equinox is a single frequency CW 532 nm laser (up to 18W). It’s inherently stable, low noise, narrow linewidth and naturally compact, robust and fully-automated.

A compact frequency conversion module that extends the range of SolsTiS output wavelengths via frequency doubling in a resonant cavity with optimised conversion efficiency.

The condenser is adjusted to match the cone of light to the numerical aperture of the objective at the working distance required. Microscope ...

The cost of additional use is $20/hour for Full and Associate Members of the Department and $40/hour for Non-members. For more training details or to schedule an appointment, please contact our Facility Manager:

Confocalfluorescence microscopy

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Continuous tuning from 700 - 1000 nm with a single optics set with custom wavelength ranges available <700 nm or >1000 nm. Optional integrated frequency conversion modules can further extend this to cover 210 – 4 µm.

Confocalmicroscopy diagram

*The Center for Ultracold Atoms (CUA) comprises a community of scientists from Harvard University and the Massachusetts Institute of Technology (MIT). The CUA is supported by the National Science Foundation (NSF).

A range of extensions are available to enhance the wavelength coverage of the system, helping you to explore new regions.

Confocallaser scanning microscopy

Appointments to use this instrument are taken using an on-line booking system. Services provided by this facility including training of new users to ensure sufficient background knowledge to successfully design experiments, operate the equipment properly and with confidence. The facility also offers technical consultation on experiment design and analysis.

Integrated extension modules are available to extend the tuning range from 210 nm to 4000 nm. These feature interlocking base plates and connecting lens tubes that enclose any beam paths not part of the final output. Beam pick off and fiber launch modules available.

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Confocalmicroscopy ppt

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Professor Charles Adams works in the Department of Physics at the University of Durham, is a Member of the Centre for Atomic and Molecular Physics and is Director of the Joint Quantum Centre.

The System Gain of a camera determines the rate of conversion between the number of electrons (e-) recorded into digital units or grey levels in an image. Gain ...

The SolsTiS External Mixing Module provides fully automated tuning in the visible (500-680 nm) and IR (1.1-4.5 µm) with further extension options into the UV (250-350 nm).

Continuous, single mode, high-resolution scans over >25 GHz, up to 300 nm (with TeraScan option). Automatically stitch together consecutive segments to achieve high-resolution scans over >100 nm with high repeatability and linearity.

M Squared’s lasers are both remarkable in their performance and beautiful in their design. They have enabled my Group’s research over the last decade and will continue to transform many areas of science and technology.

Woodchuck USA is a Saint Paul-based company that specializes in creating custom wood gifts, journals, and flasks. Their products are all made in the USA and ...

Award-winning SolsTiS is a next generation continuous-wave Ti:Sapphire laser designed to meet the needs of pioneering scientists looking for high performance, ease of use, system flexibility and reliability. This fully automated, compact system features a completely sealed, alignment-free cavity with hands-free operation, an unprecedented tuning range, unrivalled power, and the ultimate narrow linewidth, low noise output. SolsTiS has options of high power output up to 5W, linewidths <50 kHz and amplitude noise of less than 0.05%. SolsTiS is made to order giving you the ability to specify your linewidth, output power and wavelength range. Fully integrated accessories such as beam pick-off and fiber coupling are available.

Ultra stable output with relative intensity noise 0.075 % RMS with exceptional stability on longer timescales. Automatic locking of cavity elements allows continuous running over long time durations with no interruptions.

Confocal imagingprice

M Squared’s SolsTiS laser platform transformed our quantum optics experiments, helping to produce several documented breakthroughs. In fact, we were so impressed with its performance and versatility that we went on to purchase another four.

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Laser-scanning confocal microscopy is a technology that allows for high resolution, optical sectioning of individual cells in culture or embedded within tissues. The wide range of fluorescent probes and detectors enables rapid analysis of multiple parameters within one sample. The advantage of this technology over wide-field epifluorescence microscopy is that it uses point illumination to enhance image resolution and control the depth of the focal plane within tissues. The use of lasers of different wavelengths to scan tissues and excite fluorescent cell labels both horizontally and vertically allows 3D rendering of cells within whole tissues. Using this instrument, investigators can accurately measure cell size and study cell morphology, membrane and intracellular protein expression as well as cell division and death. A particular benefit of this technology is that it combines these cellular parameters with detailed information about the location of specific cell types within complex tissues. This information elucidates how intercellular interactions impact organ-specific states of health and disease. Check the tutorial on the basics of confocal immunofluorescence microscopy.