Gillespie, Claire. "What Are The Functions Of The Objective Lenses?" sciencing.com, https://www.sciencing.com/functions-objective-lenses-6470088/. 27 April 2018.

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Gillespie, Claire. (2018, April 27). What Are The Functions Of The Objective Lenses?. sciencing.com. Retrieved from https://www.sciencing.com/functions-objective-lenses-6470088/

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Gillespie, Claire. What Are The Functions Of The Objective Lenses? last modified March 24, 2022. https://www.sciencing.com/functions-objective-lenses-6470088/

Before you use a microscope, it helps to know what all the different parts are for. Many people believe that the objective lenses are the most important components of a microscope. Basically, without them, your microscope experience would be very disappointing.

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Uncertainty in charge-coupled devices (CCDs) as UV-vis spectrophotometric detectors is studied here considering that it highly affects the limit of detection of analytical methods. Opposite to photomultiplier-type detectors (PMDs) and diode-array detectors (DADs), where uncertainty is mainly dependent on the photonic signal (shot noise), in CCD detectors uncertainty may come from both independent and dependent effects upon the photonic signal. Shot noise is specially important for high photonic signals in the detector (those for low absorbances) while the uncertainty that is independent of the signal is specially important for low photonic signals in the detector (those for high absorbances). That is, the main source of uncertainty is different depending on the value of the experimental measurement. On the other hand, temperature does not practically affect absorbance measurements, though it is very important for emission measurements (fluorescence, Raman, scattering, etc.). Mathematical equations for uncertainty are proposed with excellent fittings to the experimental data. The equation parameters can be experimentally determined from non-linear regression analysis and used to characterize spectrometers or to test their performance. In order to help buyers and users, some recommendations are finally given considering, among others, cooling, slit, attenuator or fiber optic assemblies.

Most microscopes come with at least three objective lenses, which provide the majority of image enhancement. The function of objective lenses is to magnify objects enough for you to see them in great detail.

The longest objective lens is an oil immersion objective lens, which magnifies 100x. The total magnification is 1000x if the eyepiece lens is 10x power. The oil immersion objective lens is used for examining the detail of individual cells, such as red blood cells. This lens requires a special oil to form a link between the edge of the objective and the cover slip. Before you use an oil immersion objective lens, ensure the specimen is in focus under the high-power objective lens. After you remove the high-power objective, put a tiny amount of oil onto the cover slip above the specimen, and then move the oil immersion lens into position.

Every microscope has an eyepiece lens, which is the lens at the top that you look through. A tube connects the eyepiece lens to objective lenses, which enhance the magnification power of the eyepiece lens. The eyepiece lens is usually 10x or 15x power (i.e., what you look at appears to be 10 times or 15 times closer than it actually is). A rotating nosepiece or turret holds two or more objective lenses, and you can easily switch between them to change power. A microscope's stage is the flat platform that holds the slides. Some microscopes also have a condenser lens, which focuses the light onto the object, and a diaphragm or iris, which is a revolving disk with holes of varying sizes. The iris is used to vary the intensity and size of the light that is streamed upward into the slide.

A scanning objective lens that magnifies 4x is the shortest objective and is useful for getting a general overview of a slide. A low-power objective lens magnifies 10x, but remember that it is coupled with an eyepiece lens, so the total magnification is 10x times the power of the eyepiece lens. A high-power objective lens magnifies 40x, with total magnification 400x if the eyepiece lens is 10x power, and it is ideal for observing very fine detail, such as nerve cells in the retina or the striations in skeletal muscle.