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Differential interference contrastmicroscope

Additional optical microscopy applications include brightfield illumination, darkfield illumination, phase contrast, and fluorescence.

Knowledge Center/ Application Notes/ Microscopy Application Notes/ Optical Microscopy Application: Differential Interference Contrast

Differential interference contrastmicroscopy principle

Introducing a Nomarski or Wollaston prism into a DIC optical microscopy setup separates polarized light into two rays that are polarized at 90° to one another. The focal point of the prisms rests outside the glass element, and allows active focusing in the upright DIC microscope system. A second prism is required to join the two light rays back together at a polarization of 135°.

Differential interference contrastvs phasecontrast

Differential Interference Contrast (DIC) is a polarization technique in optical microscopy that uses a polarizer, analyzer, and additional polarization optics such as a Nomarski or Wollaston prism. In simple DIC setups, the only required components are a polarizer and an analyzer. The polarizer is typically positioned below the specimen, and the analyzer above the objective lens. The polarization axis of each can be rotated to adjust contrast and throughput, but the two components typically act with respect to one another.

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Figure 1 is a darkfield illumination image with specialized polarized techniques applied to it. The tissue fibers exhibit added complexity, detail, and texture as the light scattered by it is controlled and captured differently by its state of polarization. The image shows details about absorption color, optical path boundaries, and refractive indices, along with whether or not a sample is isotropic and anisotropic. Polarization techniques in optical microscopy such as DIC are invaluable in the identification of unknown samples that exhibit birefringence.