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Of course, the 395nm LED also emits at wavelengths below 395nm as well, and the light energy at these wavelengths are very effective at creating fluorescence effects or initiating UVA reactions. But, as the chart shows, it is important to notice that a significant portion of the light energy is being emitted in the visible, violet region.

When illuminating a 365nm LED, however, you will likely notice a dull, bluish-white color emitted. This is caused by visible light "leakage" where minuscule but visible amounts of visible wavelength energy (i.e. white light) are also emitted. The proportion of this visible light energy is so small that it is shown as "0" in the spectral charts, but in some applications such as UV photography, the visible light is indeed detectable and may be a nuisance. In such cases, additional visible light filtering techniques may be necessary.

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Due to the way UV LED diodes are manufactured, 365nm wavelength chips tend to be less efficient and more costly. The exact difference in price will depend on the product type, but in general, 365nm UV LEDs do come with a price premium, and as such, 395nm UV LED products are better suited for those on a budget.

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In addition to 365nm UV lights having the advantage of not emitting violet light, many objects will fluoresce strongest at 365nm, as evidenced by absorption spectrum measurements.

Conversely, looking at the 365nm spectral output, you will notice that virtually all of the light energy is within the invisible UVA range only, with emission energy tailing off before reaching 400nm. This will maximize the amount of UVA energy compared to visible light energy and is preferred for most UVA applications.

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However, the field number always assumes no magnification so to calculate the actual FOV, the field number should be divided by the objective magnification:.

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Before we get into answering the question of whether 365nm or 395nm is better for your application, it is important to understand the basic principles of wavelength and light.

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The main difference is that the 395nm LED emits much more visible light than the 365nm LED. The 395nm LED emits a pronounced violet-colored light, while the 365nm LED emits a dull, bluish-white light (the result of residual light energy that "tails" off into the visible spectrum). Both types emit in the UVA wavelength range and are generally capable of producing "blacklight" or curing effects.

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The 395nm LED emits strongest at 395nm (as defined by the term peak wavelength), but it also emits quite a bit of energy at 400nm, and even 410nm. These wavelengths are solidly in the visible, violet portion of the spectrum.

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At a basic level, then, you should note that 395 nm is about 30 nm closer to visible light (violet) than 365 nm is. Or, in other words, 365 nm is "deeper" into the UV spectrum than 395 nm is.

The result is that 365nm light may be better suited for applications where stronger fluorescence effects are desired. Coupled with the advantage of having a lower amount of visible, violet light emitted, 365nm light can be considered an optimal choice where performance is of paramount importance.

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As you may recall, different wavelengths of visible light appear as different colors to us. 450 nanometer light, for example, is a royal blue color, while 630nm is a punchy, red color. The light that has a wavelength between 400nm and 800nm is visible, while light that falls below 400nm is considered invisible, ultraviolet light.

Both 365nm and 395nm options are within the UVA wavelength range. Generally speaking, UVA lights are useful for creating and observing fluorescence effects, as well as for plastics and paint curing. UVA wavelengths are safer when compared to stronger UVB and UVC rays.

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The microscope objective is a key component for reaching high performance of a microscope. It is the part which is placed next to the observed object.

If you're looking for some UVA lights, you may come across two specifications - 365nm or 395nm. What does that mean, and which one is best? Read on to find out more!

Even though lights at wavelengths below 400nm are not visible to the human eye, the wavelength value is still nonetheless very important. For example, 365nm wavelength UV belongs to a type of ultraviolet known as "UVA," while 290nm wavelength UV is considered "UVB." The wavelength is what determines the type of UV, and this is an important distinction among UV categories in terms of application and even safety precautions that are necessary.

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Why is there a difference in the amount of visible light? The chart above shows the spectral output diagram of both the 365nm (narrow dotted line) and 395nm (wide dotted line) LEDs. What you will notice is that both 365nm and 395nm LEDs emit over a range of wavelengths both above and below their respective wavelengths. In other words, 395nm LEDs don't emit at only 395nm.