Objective lensmicroscope

The Super High Resolution USAF targets provide the finest resolution commercially available on the market. With the minimum feature as small as 137 nanometers bar width, typically these features are only easily seen with a scanning electron microscope (SEM). With microscope systems going to higher and higher resolution levels, scientists require special tools like this to help test their most demanding optical systems. A typical application would be to test a high resolution microscope at the highest magnification.  This product would facilitate the testing of the microscope systems' top resolution.  Please note that the high resolution targets are the small features in the center of the diamond aperture. The patterns in the square area on the right side of the slide are not test targests.

Objective lensmicroscope function

Choose products to compare anywhere you see 'Add to Compare' or 'Compare' options displayed. Compare All Close

Immersion Oil:  Clear, finely detailed images are achieved by contrasting the specimen with their medium.  Changing the refractive index of the specimens from their medium attains this contrast.  The refractive index is a measure of the relative velocity at which light passes through a material.  When light rays pass through the two materials (specimen and medium) that have different refractive indices, the rays change direction from a straight path by bending (refracting) at the boundary between the specimen and the medium.  Thus, this increases the image’s contrast between the specimen and the medium.

The target area is quartz with 1,000 angstroms of chrome. The quartz substrate is fastened to the metal slide with UV curing epoxy.  Rarely, microscopic amounts of blocking wax may be present post manufacture.  If this is the case, please remove the fragments with D-limonene.  Acetone can work in this case.  However, be wary of using too much which may loosen the epoxy.  Soap and water will also work or any other cleaning agent used to clean microscope objective immersion oil. Do not soak in any solvent. See optic cleaning instructions for additional information.

Objective lens magnificationformula

The target area is quartz with 1,000 angstroms of chrome. The quartz substrate is fastened to the metal slide with UV curing epoxy.  Rarely, microscopic amounts of blocking wax may be present post manufacture.  If this is the case, please remove the fragments with D-limonene.  Acetone can work in this case.  However, be wary of using too much which may loosen the epoxy.  Soap and water will also work or any other cleaning agent used to clean microscope objective immersion oil. Do not soak in any solvent. See optic cleaning instructions for additional information.

If you are not testing a microscope and need to mount the resolution target in free space, the M-PPF50 mount works very well to mate to the HIGHRES targets.  There are two springs that can easily hold the HIGHRES target in place. Please note that the mount is metric, so the mount will only mate to a metric post. The posts, whether metric or english, will have the same diameter, so the post will be able to fit a metric or english post holder. For other mounting options, please check out spring-loaded optical filter mounts.

A typical application would be to test a high resolution microscope at the highest magnification.  This product would facilitate the testing of the microscope systems' top resolution.

Ocularlens magnification

One way to change the refractive index is by staining the specimen.  Another is to use immersion oil.  While we want light to refract differently between the specimen and the medium, we do not want to lose any light rays, as this would decrease the resolution of the image.  By placing immersion oil between the glass slide and the oil immersion lens (100X), the light rays at the highest magnification can be retained.  Immersion oil has the same refractive index as glass so the oil becomes part of the optics of the microscope.  Without the oil the light rays are refracted as they enter the air between the slide and the lens and the objective lens would have to be increased in diameter in order to capture them.  Using oil has the same effect as increasing the objective diameter therefore improving the resolving power of the lens.

Objective lens magnificationexample

Please take a few minutes to fill out a brief survey about your experience using the Virtual Edge: https://docs.google.com/forms/d/1yGbkF0KM92WBSk-IgS-EkjxkTKTQwhzuXmDsVpwRDoU/viewform

Low powerobjective lens

The Super High Resolution USAF targets provide the finest resolution commercially available on the market. With the minimum feature as small as 137 nanometers bar width, typically these features are only easily seen with a scanning electron microscope (SEM). With microscope systems going to higher and higher resolution levels, scientists require special tools like this to help test their most demanding optical systems.

Resolving power or resolution: the ability to distinguish objects that are close together.  The better the resolving power of the microscope, the closer together two objects can be and still be seen as separate.

Total magnification: In a compound microscope the total magnification is the product of the objective and ocular lenses (see figure below).  The magnification of the ocular lenses on your scope is 10X.

Low powerobjective magnification

Parfocal: the objective lenses are mounted on the microscope so that they can be interchanged without having to appreciably vary the focus.

The Virtual Edge by http://www.uwyo.edu/virtual_edge/ is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 United States License

High powerobjective lens magnification

Yes, opt-in.  By checking this box, you agree to receive our newsletters, announcements, surveys and marketing offers in accordance with our privacy policy

If you are not testing a microscope and need to mount the resolution target in free space, the M-PPF50 mount works very well to mate to the HIGHRES targets.  There are two springs that can easily hold the HIGHRES target in place. Please note that the mount is metric, so the mount will only mate to a metric post. The posts, whether metric or english, will have the same diameter, so the post will be able to fit a metric or english post holder. For other mounting options, please check out spring-loaded optical filter mounts.

Element Number Group Number 4 5 6 7 8 9 10 11 1 31.250 15.625 7.813 3.906 1.953 0.977 0.488 0.244 2 27.841 13.920 6.960 3.480 1.740 0.870 0.435 0.218 3 24.803 12.402 6.201 3.100 1.550 0.775 0.388 0.194 4 22.097 11.049 5.524 2.762 1.381 0.691 0.345 0.173 5 19.686 9.843 4.922 2.461 1.230 0.615 0.308 0.154 6 17.538 8.769 4.385 2.192 1.096 0.548 0.274 0.137

Element number is the number left or right to the lines. Group number is the number on top of each area. Element Number Group Number 4 5 6 7 8 9 10 11 1 16.0 32.0 64.0 128.0 256.0 512.0 1,024 2,048 2 18.0 35.9 71.8 143.7 287.4 574.7 1,149 2,299 3 20.2 40.3 80.6 161.3 322.5 645.1 1,290 2,580 4 22.6 45.3 90.5 181.0 362.0 724.1 1,448 2,896 5 25.4 50.8 101.6 203.2 406.4 812.7 1,625 3,251 6 28.5 57.0 114.0 228.1 456.1 912.3 1,825 3,649

Mounted in a black anodized aluminum microscope slide and created with nanometer scale elements, the Super High Resolution USAF Test Targets are the ideal testing solution for high resolution microscope systems.

Please note that the high resolution targets are the small features in the center of the diamond aperture. The patterns in the square area on the right side of the slide are not test targests.

Mounted in a black anodized aluminum microscope slide and created with nanometer scale elements, the Super High Resolution USAF Test Targets are the ideal testing solution for high resolution microscope systems.