The spelling transwoman (written as a single word) is occasionally used interchangeably with trans woman (where trans is an adjective describing a kind of woman). However, this variant is often associated with views (notably gender-critical feminism) that exclude trans women from women, and thus require a separate word to describe them.[12] For this reason, many transgender people find the spelling offensive.[12][13] Some prefer to omit trans, and be called simply women.[10]

In 2015, a false statistic was widely reported in the United States media stating that the life expectancy of trans women of color is only 35 years.[64] This appears to be based on a comment specifically about Latin America in a report by the Inter-American Commission on Human Rights, which compiled data on the age at death of murdered trans women for all of the Americas (North, South, and Central), and does not disaggregate by race.[64][65][66]

The European study found that sexual orientation did not change over the 12 months.[28] A 2018 study found that the most common sexual partner for trans women was cisgender women prior to transitioning. Trans women who had been for transitioning for ten years or more were more likely to report a shift in their sexual orientation.[29]

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A 2011 survey of roughly 3000 trans women living in the United States, as summarized in the report "Injustice at Every Turn: A Report of the National Transgender Discrimination Survey", found that trans women reported that:[26][specify]

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Trans women vary greatly in terms of sexual orientation.[22][23][24][25] A survey of roughly 3,000 American trans women showed 31% of them identifying as bisexual, 29% as "gay/lesbian/same-gender", 23% as heterosexual, 7% as asexual, as well as 7% identifying as "queer" and 2% as "other".[26] A 12-month survey of trans women in Europe found that 22% identified as heterosexual, 10% were attracted almost exclusively to men, 3% were mostly attracted to men, 9% were bisexual, 7% were mostly attracted to women, 23% were almost attracted exclusively to women, and 20% were lesbian. A smaller 2013 study of Italian trans women found that 82% identified as heterosexual.[27]

Both excitation filters and emission filters play crucial roles in fluorescence microscopy; they have distinct functions and target different aspects of light:

Trans women may undergo a variety of gender-affirming surgeries as part of their transition process. These surgeries may include vaginoplasty, vulvoplasty, orchiectomy, breast augmentation, and facial feminization surgery.[45]

Usually set at a 45-degree angle, the dichroic filter reflects and transmits light in specific bands for excitation and emission. It’s also handy for separating different light colours while keeping out unwanted light.

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Trans women may elect to undergo fertility preservation through semen cryopreservation via masturbation or testicular sperm extraction.[46]

Trans women face significant discrimination in many areas of life—including in employment and access to housing—and face physical and sexual violence and hate crimes, including from partners. In the United States, discrimination is particularly severe towards trans women who are members of a racial minority, who often face the intersection of transmisogyny and racism.

Discrimination against trans women has occurred at the Michigan Womyn's Music Festival after the Festival set out a rule that it would only be a space for cisgender females. This led to protests by trans women and their allies, and a boycott of the Festival by Equality Michigan in 2014. The boycott was joined by the Human Rights Campaign, GLAAD, the National Center for Lesbian Rights, and the National LGBTQ Task Force. The "womyn-born-womyn" intention first came to attention in 1991 after a transsexual festival-goer, Nancy Burkholder, was asked to leave the festival when several women recognized her as a trans woman and expressed discomfort with her presence in the space.[58][59]

Some trans women may seek to feminize their voice through transgender voice therapy, as hormone therapy does not significantly affect the voice of trans women. The aim of voice therapy (in the context of transitioning) is frequently to change the fundamental frequency, resonant frequency, and phonatory pattern to reflect that of cisgender women.[42] This can be accomplished through speech therapy, or surgeries (including feminization laryngoplasty). Throughout multiple studies, voice therapy has generally been shown to increase vocal satisfaction of the patient and a greater listener perception of a feminine voice.[43][44]

The microscope has filters to let in only the light that matches the glowing material. This light hits the atoms in the sample and makes them give off light, too. To see this light better, another filter blocks out the intense light used for lighting up the sample.

Transfeminine (or transfemme) is a broader umbrella term for assigned-male trans individuals with a predominantly feminine identity or gender expression. This includes trans women, but is used especially for AMAB non-binary people, who may have an identity that is partially feminine, but not wholly female.[11]

The VLP 5 is a visible red laser source designed for detecting and highlighting breaks, tight bends, splices, or defective connectors.

In her book Whipping Girl, trans woman Julia Serano refers to the unique discrimination trans women experience as "transmisogyny".[57]

The term transgender women is not always interchangeable with transsexual women, although the terms are often used interchangeably. Transgender is an umbrella term that includes different types of gender variant people (including transsexual people).

While the relationship is not completely understood,[46] feminizing hormone therapy appears to reduce the ability to produce sperm.[47] Individuals who have been on hormone therapy for an extended period of time have been shown to have a lower total sperm count than males not on hormone therapy.[48] Cessation of hormone replacement therapy has been associated with a renewed level of fertility.[49][50]

Trans representation in television, film, news, and other forms of media was slim before the 21st century. Early mainstream accounts and fictional depictions of trans women almost always relied on common tropes and stereotypes.[69] However, portrayals have steadily grown and improved in tandem with activism.

Feminizing hormone therapy is a type of hormone therapy focused on turning the secondary sex characteristics of a person from masculine to feminine. Feminizing hormone therapy often includes a mix of estrogens, antiandrogens, progestogens, and gonadotropin-releasing hormone modulator,[1][39] though the most common approach is an estrogen in combination with an antiandrogen.[40][41] Feminizing hormone therapy can induce effects including breast development, softening of the skin, redistribution of body fat towards a gynoid fat distribution, decreased muscle mass/strength, and changes in mood.

Analogy: Imagine editing a picture at a party, removing the flashing lights (excitation light) and keeping only the colours of the glowing objects (emission wavelengths), allowing you to see them.

These are the tiny guests at the party. They only get excited by specific colours of light (excitation wavelengths). When the allowed colours reach the fluorophores, they get “excited” and start glowing with their different colours (emission wavelengths). After the fluorophores get excited, another filter (emission filter) helps us see them by blocking out the original light (like turning off the disco ball).

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They stick fluorescent tags onto molecules in cells, like proteins or DNA, to see where they are and how many there are. This helps figure out how cells are organized and find particular parts.

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Scientists can attach unique tags to things they want to see and use filters to make only those things show up in the picture. Confocal fluorescence microscopy is used a lot to show 3D stuff. It uses intense light, like lasers, to focus on tiny points in the sample. Then, it puts all those points together to make a 3D picture.

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Gender-affirming care for trans women may include feminizing hormone therapy, transgender voice therapy, and gender-affirming surgery (often referring to vaginoplasty, but may also include tracheal shave, orchiectomy, facial feminization surgery, breast augmentation, and vulvoplasty).[38]

Tucking is also associated with lower quality sperm production because of the increased temperature of the testicles, causing premature sperm death.[51][52][53]

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One type of violence towards trans women is committed by perpetrators who learn that their sexual partner is transgender, and feel deceived ("trans panic"). Almost 95% of these crimes were committed by cisgender men towards trans women.[68] According to a 2005 study in Houston, Texas, "50% of transgender people surveyed had been hit by a primary partner after coming out as transgender".[62]

In Thailand, kathoey refers to a trans-feminine individual, though the term "transgender" is infrequently used to refer to those with this identity.[16] The term is sometimes translated to "ladyboy" in English.[17] Most trans-feminine Thai individuals simply referred to themselves as women, or phuying praphet song, meaning "another type of woman."[18]

Trans women face a form of violence known as trans bashing. The Washington Blade reported that Global Rights, an international NGO, tracked the mistreatment of trans women in Brazil, including at the hands of the police.[60] To commemorate those who have been murdered in hate crimes, an annual Transgender Day of Remembrance is held in various locations across the United States, Canada, Western Europe, Australia, and New Zealand, with details and sources for each murder provided at their website.[61]

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In several Latin American countries, the word travesti is sometimes used to designate people who have been assigned male sex at birth, but develop a female gender identity. The use of travesti precedes transgender in the region; its distinction from trans woman is controversial and can vary depending on the context, ranging from considering it a regional equivalent to a third gender.[14][15]

More light transmission for brighter signals but also more background noise. Less light throughput but improved signal specificity (distinguishing between close-emitting fluorophores). If using many fluorophores, select filter sets designed to cut spectral overlap and ease the clear separation of signals from each fluorophore.

According to Trans Murder Monitoring, between Oct 1, 2022 and September 30, 2023, 321 trans and gender-diverse individuals were killed, with trans women or trans-feminine individuals accounting for 94% of the deaths.[63]

Optics filters play a vital role in controlling the wavelengths of light that interact with specimens in optical instruments. Excitation filters allow specific wavelengths of light to illuminate a sample. At the same time, emission filters unwanted wavelengths from the emitted light. This article compares excitation and emission filters, elucidating their functions, types, and applications.

They can use fluorescently labelled antibodies to find harmful things like bacteria or viruses in cells or tissues. This helps diagnose diseases and see how pathogens affect the body.

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They can watch molecules move around and interact in real time. This helps us understand how proteins move around in cells, what organelles do, and how cells divide.

Excitation and emission filters are like the superheroes of fluorescence microscopy. They help scientists see tiny things in cells and tissues by making specific molecules glow. These filters are crucial for spectroscopy systems, fancy machines used in biology and medicine to study these glowing molecules.

An excitation filter is like a colour-coded bouncer in light and science. It’s used in special microscopes to study tiny, glowing molecules called fluorophores. Imagine a bright light shining like a party disco ball, with all different colours of light mixed. This filter only lets in specific light colours, like letting people wearing blue shirts into the party. These particular colours are called excitation wavelengths.

Choose an excitation filter centred around the peak absorption wavelength of your fluorophore. An emission filter centred around your fluorophore’s peak emission wavelength. Consider the difference between the absorption and emission peaks (Stokes shift) to cut overlap. And ensure the best signal separation.

The dichroic beamsplitter, also known as a dichroic mirror, is a filter that limits the angle at which light can be viewed to certain parts of the electromagnetic spectrum. It lets through specific wavelengths while blocking others.

According to a 2009 report by the National Coalition of Anti-Violence Programs, quoted by the Office for Victims of Crime, 11% of all hate crimes towards members of the LGBTQ community were directed towards trans women.[62]

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Most fluorescence microscopes in biology shine light on the sample from above and look at the glow from above, too. They often use bright lamps like Xenon or Mercury lamps.

Function: Acts like a finishing touch artist, blocking out the remaining excitation light and allowing only the desired emission wavelengths (different colours of light) emitted by the excited fluorophores to reach the detector.

In the 2020 film Disclosure: Trans Lives on Screen, director Sam Feder explores Hollywood's history of trans representation and the cultural effects of such depictions. Many notable 21st century trans actresses and celebrities shared their stories in the film, including Laverne Cox, Alexandra Billings, Jamie Clayton, AJ Clementine, and more.[70]

Gender-affirming care may include social or medical transition. Social transition may involve changes such as adopting a new name, hairstyle, clothing style, and/or set of pronouns associated with the individual's affirmed gender identity.[2] A major component of medical transition for trans women is feminizing hormone therapy, which causes the development of female secondary sex characteristics (breasts, redistribution of body fat, lower waist–hip ratio, etc.). This, along with socially transitioning, and receiving desired gender-affirming surgeries can relieve the person of gender dysphoria.[3][4] Like cisgender women, trans women may have any sexual orientation.

The primary filters in fluorescence microscopy are mounted in specific cubes. In modern microscopy technology, these filters are often interference filters to improve imaging quality and precision.

Excitation and emission filters are essential in optical systems, particularly in fluorescence microscopy and spectroscopy. Understanding the differences between these filters is crucial for optimizing experimental setups and achieving accurate results.

It’s like editing a photo taken at a party, where the emission filter removes the flashing lights and highlights only the colours of the hats people wear, allowing you to see them. So, the emission filter is essential for improving the image by eliminating unwanted background noise. And showcase the specific glowing molecules scientists want to study.

Some famous trans women in television include Laverne Cox (playing Sophia Burset on Orange is the New Black), Hunter Schafer (playing Jules Vaughn in Euphoria), Josie Totah, and Caitlyn Jenner (from Keeping Up with the Kardashians).[71][72] Pose, an American television show, depicts the lives of several trans women.[73]

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It acts like a spotlight, only letting through the colours emitted by the excited fluorophores while blocking out all the other light. This creates a clear view of the glowing molecules against a dark background, making it easier for scientists to study them.

Function: An excitation filter works like a gatekeeper for light entering the sample. It only lets certain types of light through, called excitation wavelengths. These wavelengths are picked because they get absorbed by the glowing molecules (fluorophores) in the sample, making them excited.

A fluorescence microscope is like a regular microscope but has extra features to improve it. Instead of normal light, it uses bright light to make certain parts of a sample glow. This glow helps us see tiny things like germs or makes 3D features more accessible.

Trans women may also be short for transsexual women. Transsexual is a subset of transgender,[8][9] referring to people who desire to medically transition to the sex with which they identify, usually through sex reassignment therapies, such as hormone replacement therapy and sex reassignment surgery, to align their body with their identified sex or gender. The term is rejected by some as outdated, though others within the trans community still identify as transsexual.[10]

In fluorescence microscopy, the emission filter is like a particular artist putting the final touches on a painting. Once the excitation filter has excited the tiny glowing molecules (fluorophores) with specific light colours, the emission filter steps in.

Scientists can watch how drugs affect cells under a microscope in real time. This helps them develop new medicines and see how they work in cells.

In 2016, 23 transgender people suffered fatal attacks in the United States. The Human Rights Campaign report found some of these deaths to be direct results of an anti-transgender bias, and some due to related factors such as homelessness.[67]

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Amongst Native Hawaiians and Tahitians, māhū are people of a third gender who possess spiritual and social roles.[19] The term has historically been applied to people assigned male at birth, but now may refer to a large variety of gender identities.[20] The term is sometimes seen as disparaging or a pejorative, similar to faggot.[21]

Consider the type of light source (e.g., mercury lamp, LED) and any existing limitations in your microscope’s configuration.

Transgender (commonly abbreviated as trans)[5] is an umbrella term for people whose gender identity or gender expression are different from those typically associated with members of the sex they were assigned at birth.[6] Transgender women, sometimes called male-to-female (MTF, M2F), are those who were assigned the male sex at birth (AMAB), but who identify and live as women.[7]

By understanding how these work, scientists can make sure they get accurate data. This helps them know how cells and molecules behave, which can lead to exciting discoveries.

In a 2008 study, no statistically significant difference in libido was detected between trans women and cisgender women.[30] As in males, female libido is thought to correlate with serum testosterone levels[31][32][33][34] (with some controversy)[35] but the 2008 study found no such correlation in trans women.[30][36] Another study, published in 2014, found that 62.4% of trans women reported their sexual desire had decreased after sexual reassignment therapy.[37]

Like all gender variant people, trans women often face discrimination and transphobia,[26]: 8  particularly those who are not perceived as cisgender.[54] A 2015 survey from The Williams Institute found that, of 27,715 transgender respondents, 52% whose families had rejected them attempted suicide, as did 64.9% of those who were physically attacked in the past year.[55]

Analogy: Think of an excitation filter like a bouncer at a nightclub. This bouncer only lets people wearing specific coloured shirts (representing excitation wavelengths) enter the club. These particular colours are chosen because they excite the glowing molecules (like partygoers) inside, making them lively and bright.

By using different colours of fluorescent tags, scientists can label and tell apart different types of cells in one sample. This is handy for studying tissues with lots of different cell types.

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The sample is usually tagged in a fluorescence microscope with a particular substance called a fluorophore. Then, it’s lit up with intense light. The fluorophores absorb this light, making them glow with a different, weaker light. Filters in the microscope only let this softer light through, so you can see what’s glowing.

Transgender women (often shortened to trans women) are women who were assigned male at birth. Trans women have a female gender identity and may experience gender dysphoria (distress brought upon by the discrepancy between a person's gender identity and their sex assigned at birth).[1] Gender dysphoria may be treated with gender-affirming care.

The American National Coalition of Anti-Violence Programs' report of 2010 anti-LGBTQ violence found that of the 27 people who were murdered because of their LGBTQ identity, 44% were trans women.[56] Discrimination is particularly severe towards non-white trans women, who experience the intersection of racism and transphobia.

Choosing suitable filters is super important. It’s like picking the perfect outfit for a special occasion. Scientists need to consider things like the colour of the molecule. They’re studying the brightness they need and how much background noise they want to block out.

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