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The condenser is what I was referring to badly. You can optimized the light so that it focus in the peak plane for your specific objective and broaden after that point to just fill the exact diameter of your specific objective front glass opening. That would be highly customized to your objective lens and give you the best images.

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Just play with it and enjoy it. You first ruler shot is a great start. Just focus on learning how to focus it and give it more light than you think.

I'll probably be doing mostly reflected light, but it is nice to know what is needed for light transmitted through the specimen.

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A microscope has a light source, a diaphragm (controls the shape and amount of light like a lens aperture), a condenser (a lens that focuses that light on to the subject at the peak focus point for your objective), then a sample holding stage and your objective.

This covers 8mm x 6mm on a full frame camera & is a very simple set-up with a 160mm x M42 tube length & the camera on a focusing rail.

Forth & last, sorry for the poor image but the wind did it. At least it proves the 4x microscope objective is UVA capable, as the camera is.

Field curvature is normally not that important either for our rather 3-dimensional motifs that need photo-stacking anyway.

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Going the photomacrographic way, the Laowa 25mm f/2.8 provides 2.5-5X magnification with very good image quality. The Mitakon 20mm f/28 is much cheaper but only gives 4-4.5X.

A great Nikon or Leitz or Zeiss objective will cost hundreds to thousands just for the objective. But my microscope science career is mostly over and its time for fun.

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Some very poor images unfortunately as it was windy, but I wanted to see what to expect & particularly how the 4x microscope objective preforms in UVA, which I am pleased with.

A finite focus microscope objective relies upon the secondary optical system, the ocular, to give flat field correction.

Most finite Nikon Plan microscope lenses have most of their optical correction already in the objectives, making them more suited to be used as camera objectives.