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Other wavelengths are also possible to be measured, however the specifications might change because all trials were conducted using 1050-1070 nm lasers.

Each optical path through the LBS-300HP-NIR provides uniform attenuation of any beam shape (Gaussian, flat-top, doughnut, etc.) while preserving the polarization and overall profile of the incoming laser beam thus providing accurate sample of incident beam. A 1.035-40 thread is provided behind each wedge along the axis of the output beam. These can be used to directly mount accessories with 1” lens tubes such as beam dumps or power/energy sensors.

Less than 0.0001% (1/10⁶) of the beam is reflected in one direction and less than 0.1 % in another while remaining 99.9% of incident laser beam is transmitted.

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Generally, any beam dump can be used. We recommend using Ophir Beam Dumps with high absorbance at 1070nm and locating it at least 10cm from LBS-300HP to avoid scattered light reaching beam profiler CCD.

Regular laser safety precautions should apply. Please note, that the main incident beam will exit the LBS-300HP refracted approximately 6°. Provision must be made to safely contain the transmitted beam by beam dump or power meter.

We recommend operating and storing the LBS-300HP in dust-free conditions, like a regular office or laboratory environment.Dust and other volatile particles on the wedge surface might cause reflection change and influence the measured beam profile.Harsh industrial environment operations will require a chamber for isolation.

The term fluorescence refers to one type of luminescence. Luminescence, broadly defined, is light emission from a molecule. There are several types of luminescence.

Fluorescence spectroscopy is an investigative method based on the fluorescence properties of the sample under study, and is used for quantitative measurements of chemical products.

Fluorescent molecules and materials come in all shapes and sizes. Some are intrinsically fluorescent, such as chlorophyll and the amino acid residue tryptophan (Trp), phenylalanine (Phe) and tyrosine (Tyr). Others are molecules synthesized specifically as stable organic dyes or tags to be added to otherwise non-fluorescent systems. There are entire catalogs of these available. Typically, organic fluorescent molecules have aromatic rings and pi-conjugated electrons in them. Depending on their size and structure, organic dyes can emit from the UV out into the near-IR.

These two spectral types (emission and excitation) are used to see how a sample is changing. The spectral intensity and or peak wavelength may change with variants such as temperature, concentration, or interactions with other molecules around it. This includes quencher molecules and molecules or materials that involve energy transfer. Some fluorophores are also sensitive to solvent environment properties such as pH, polarity, and certain ion concentrations.

Polarizing filter (photography) ... A polarizing filter or polarising filter (see spelling differences) is a filter that is often placed in front of a camera lens ...

A specification defines beam attenuation of a million, meaning less than 0.0001% (1/10⁶) of the beam is reflected towards beam profiler or power meter, however, this parameter can vary from unit to unit. In the majority of units, the reflection is even less than 0.0001%. The median reflection is 0.000075% and can vary by +/-20% (relative). The exact reflection from each surface is measured during the final production QC and documented in COC supplied with the unit. You can also verify the real reflection of your unit with Ophir support team.

Steady state fluorescence spectra are when molecules, excited by a constant source of light, emit fluorescence, and the emitted photons, or intensity, are detected as a function of wavelength. A fluorescence emission spectrum is when the excitation wavelength is fixed and the emission wavelength is scanned to get a plot of intensity vs. emission wavelength.

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In case of surface contamination, we recommend contactless cleaning of wedge surface by rinsing it with a chemically pure solvent such as Ethanol, Methanol, or IPA (Isopropyl Alcohol), followed by prolonged gentle nitrogen or pure compressed air purging.

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Electroluminescence, is when electrical energy or a strong electric field, stimulates the emission of a photon, such as in some lighting applications.

If contamination is not removed after cleaning, please consider whether it interferes with your laser beam. The spot might be peripheral and not in CA of the laser.

Due to such significant attenuation, some light scattered from wedges might interfere with correct beam profile measurement and cause offset. Thus, in case the setup allows it we recommend distancing the CCD from wedges by 50, 75, or 100 mm C-mount extension tube between LBS-300HP and beam profiler.

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Any contact with UVFS wedges installed in the LBS-300HP assembly can cause damage and reflection change resulting in beam profile distortion. Thus, it's recommended to avoid even the slightest contact with glass surface with neither hand, paper, or cloth.

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Fluorescence, specifically, is a type of photoluminescence where light raises an electron to an excited state. The excited state undergoes rapid thermal energy loss to the environment through vibrations, and then a photon is emitted from the lowest-lying singlet excited state. This process of photon emission competes for other non-radiative processes including energy transfer and heat loss.

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Operating and storing of LBS-300HP at conditions free of volatile particles, like a regular office or laboratory environment. Harsh industrial environment operations will require isolation.

Fluorescence spectroscopy uses a beam of light that excites the electrons in molecules of certain compounds, and causes them to emit light. That light is directed towards a filter and onto a detector for measurement and identification of the molecule or changes in the molecule.

Other wavelengths are also possible to be measured, however the specifications might change because all trials were conducted using 1050-1070 nm lasers.

Operating and storing of LBS-300HP at conditions free of volatile particles, like a regular office or laboratory environment. Harsh industrial environment operations will require isolation.

After removal, an additional laser output, having less than 0.1% of incident beam power will be reflected. That beam could be used for simultaneous measurement of laser power via Ophir Power Meter in addition to beam profiler.

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When the term “fluorescence” is used, the same methods of measurement can typically be applied to any of the above categories of luminescence.

Chemiluminescence, is defined as when chemical energy stimulates the emission of a photon, and this includes bioluminescence, as seen in fire flies and many forms of sea life.

The LBS-300HP-NIR is a patent pending technology beam splitter for high power lasers that allows measuring NIR (~1064 nm) focused or collimated laser beam profiles up to 5 kW or 15 MW/cm². It operates by reflecting a fraction of the incoming beam through the front surface of each of a pair of orthogonally oriented wedges. Less than 0.0001% of the beam is reflected towards the Beam Profiler Camera.

Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state.

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The beam blocker is designated to keep one wedge surface protected during handling and shipping. It can be left attached for low power laser operations. However, in the case of high-power laser irradiation scattered light might interfere with beam profile measurement and cause offset. In case of such offset, we recommend removing the beam blocker.

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The beam splitter is designated both for CW and pulsed lasers. The allowed energy density of 1064 nm pulsed laser should not exceed the equivalent of 15 MW/mm².

Here are a random sampling of a few common fluorophores that span the UV and Visible range. Some rare earth elements, or lanthanides, have higher electronic orbitals filled, where electrons transition due to metal ligand charge transfers happen between 4f-5d and even 4f-4f orbitals. (Bunzli, 1989) There are many molecules that are luminescent in nature such as a few of the amino acids, chlorophylls, and natural pigments. Others are highly engineered for very specific uses of fluorescence spectroscopy.

Generally, continues usage of LBS-300HP is limited only by temperature increase, thus in case the temperature is mild, there is no limitation.

The beam splitter is designated both for CW and pulsed lasers. The allowed energy density of 1064 nm pulsed laser should not exceed the equivalent of 15 MW/mm².

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Other molecules and materials such as fluorescent proteins, semiconductors, phosphors, and rare earth elements are among the commonly used fluorescent samples. Polymers with conjugated aromatics or dienes also commonly have fluorescent properties. Of course, new materials are being created all the time.

Less than 0.0001% (1/10⁶) of the beam is reflected in one direction and less than 0.1 % in another while remaining 99.9% of incident laser beam is transmitted.

If contamination is not removed after cleaning, please consider whether it interferes with your laser beam. The spot might be peripheral and not in CA of the laser.

A fluorescence excitation spectrum is when the emission wavelength is fixed and the excitation monochromator wavelength is scanned. In this way, the spectrum gives information about the wavelengths at which a sample will absorb so as to emit at the single emission wavelength chosen for observation. It is analogous to absorbance spectrum, but is a much more sensitive technique in terms of limits of detection and molecular specificity. Excitation spectra are specific to a single emitting wavelength/species as opposed to an absorbance spectrum, which measures all absorbing species in a solution or sample. The emission and excitation spectra for a given fluorophore are mirror images of each other. Typically, the emission spectrum occurs at higher wavelengths (lower energy) than the excitation or absorbance spectrum.

For the most comprehensive book on Fluorescence Spectroscopy please read Dr. Joseph Lakowicz’s book, Principles of Fluorescence Spectroscopy, third edition.

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According to tests, after 10 minutes at 2kW lasing the temperature is increased by 16 ºC and after 4 kW lasing increased by 23.4 ºC.

Generally, continues usage of LBS-300HP is limited only by temperature increase, thus in case the temperature is mild, there is no limitation.

The LBS-300HP-NIR uses specially treated material that provides extremely low reflection and high laser damage threshold, enabling beam attenuation by around a million times while keeping all beam parameters. This extreme attenuation enables measuring High Power laser beam profile focused on a beam profiler CCD.

According to tests, after 10 minutes at 2kW lasing the temperature is increased by 16 ºC and after 4 kW lasing increased by 23.4 ºC.

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