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A common question when viewing a microscopy image on a large screen is: What is the total magnification I am viewing on my monitor?" In order to correctly calculate the answer to this question, we must know the answer to the following four questions.

If you have questions regarding calculating on-screen microscope magnification contact Microscope World and we will be happy to help.

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The next number we need to determine is the diagonal measurement of the monitor in mm (3). Typically a monitor's measurement is provided in inches. We will just simply convert the inches measurement to mm. For example, if you are using a 19" monitor, multiply this number by 25.4 to convert it to mm. 19" x 25.4 = 482.6mm diagonal monitor measurement.

The microscope c-mount adapter (2) is the piece used to connect the microscope and the microscope camera. The c-mount adapter will have a number printed on the side of it. In the image at right, the c-mount adapter has a 0.5x lens built into it.

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There are several haze removal products out there that can help with leftover ghost images.  Please keep in mind that haze removers tend to be pretty harsh chemicals, so you want to be careful while using them.  Certain industrial strength haze removers can eat through mesh if left on a screen too long, so don’t step away while dealing with ghost images. Whenever using a dehazer, make sure you follow the directions for it precisely.

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Partially underexposed emulsion is a very common cause for ghost images.  When put in contact with certain clean up chemicals, underexposed emulsion can become locked into the knuckles of the mesh, making it very difficult to remove. Be aware of what chemicals you’re using, and always try to avoid underexposing screens.  Although a slightly underexposed screen can be used without any apparent problem, come reclaim time, you may find yourself with a stubbornly locked screen.

We recommend the use of our Sgreen Stuff Dehazer and Degreaser.  Sgreen Stuff does the job of two chemicals, both a dehazer and a degreaser.  It uses a unique pumice additive that cleans the mesh of stains, and oil and contaminants at the same time, leaving you with a fully reclaimed and ready to use screen while being more environmentally friendly and easy on the nose.

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Aside from an underexposed screen, there are a number of other causes for ghost images. If your screen has low tension, you’ll need to set your off contact higher, which means you’ll end up using excessive pressure to get a good print. This can trap pigments in the mesh. The use of abraders during your screen prep can cause scratches in the surface of the mesh that creates areas for pigments to settle. Also, very similarly to underexposed emulsion, if you do not dry your screen properly prior to coating, you can end up with an emulsion ghosting.

Hazes, or ghost images, can happen to the best of us, but by paying attention to your stencil preparation, exposure process, and what chemicals you’re using, you can avoid the worst of them.

A ghost image is a stain in the mesh of the screen. This can occur from either the ink or emulsion used to create the image. Most printers experience both types of ghost image.  In both cases, there can be several reasons for the ghosting.

The microscope objective lens magnification is printed on the side of the lens as shown in the image at left (1). If you are using a stereo microscope the objective lens value would be printed on the zoom knob or on the objective turret that is turned to change the microscope magnification.

The final number we will need in order to determine the on-screen magnification is the size of the camera sensor in mm (4). The size of the sensor differs slightly from the size of the chip, so the best way to find this number is to use the chart located below. The microscope camera sensor sizes are listed at the top of the chart, and the corresponding diagonal sensor size in mm is shown below.

If we are using the 19" monitor we mentioned earlier, to convert this to mm we multiple 19 x 25.4 = 482.6mm screen size.  For this example, let's say we are using a microscope camera with a 1/2" camera sensor in it. Based on the chart above we would calculate digital magnification by using 482.6mm / 8.00 = 60.325.

Now we can find the total on-screen magnification by multiplying optical magnification x digital magnification. In our example 2 x 60.325 = 120.65x on-screen magnification.