Oil immersionobjective microscope function

Condenser Lens: The purpose of the condenser lens is to focus the light onto the specimen. Condenser lenses are most useful at the highest powers (400x and ...

Nosepiecemicroscope function

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Your eyes are amazing organs. Did you know if you have blue eyes, you share an ancestor with all other blue-eyed people on the planet? That’s because blue eyes are a genetic mutation that appeared more than 6,000 years ago. All humans originally had brown eyes.

What isobjectivelens inmicroscope

Low Power Objective (10X): This next shortest objective is probably the most useful lens for viewing slides.  Almost any feature you need to observe in this course can be located with the 100X total magnification this objective provides.  As an added benefit, the low power objective is always safe to use as it cannot be lowered to the point of contacting and thus possible breaking a slide.  This is also true of the scanning objective mentioned above.

Objectivelens magnification

High Power Objective (40x): This objective (sometimes called the "high-dry" objective) is useful for observing fine detail such as the striations in skeletal muscle, the arrangement of Haversian systems in compact bone, types of nerve cells in the retina, etc.

A literal illusion is when the image you see is different from the images that make it up. For example, the illustration Hill created which is actually two images strategically drawn to look like one fluid image is a literal illusion. Think of it as a reversible figure. The end result you see in a literal illusion is based on your perception. Both images exist.

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Scanning Objective (4X): This shortest objective is useful for getting an overview of the slide (especially handy with some of the slides that contain whole organs like a section of the spinal cord, lung, digestive tract, ovary, etc.).

Stage clipsmicroscope function

There are three main types of optical illusions including literal illusions, physiological illusions and cognitive illusions. All three types of illusions have one common thread.  The perception of the image given to the brain doesn’t measure up. That’s why optical illusions are referred to as a “trick” of the eye.

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Scanningobjectivelens

These types of optical illusions are more complex because they reply on the over stimulation of the brain’s senses. The eye “sees” so much light, movement, color, dimension and size that it confuses the brain. The mind benders you see like geometric illusions and impossible pictures are physiological optical illusions.  At first glance, a two-dimensional figure looks like it’s three-dimensional.  That’s because the brain immediately interprets it to be that way. Upon further investigation, the brain realizes what the eye is actually seeing.  The image in question does not exist in nature.

If you take a step beyond the interesting stories your eyes tell, you will discover how they actually work. Your eyes work in constant unison with your brain, which is why you see the world as you do.

Although your microscope may differ in some details from the one described in the web page above, most microscopes will have one or more of the following objective lenses.  You should acquaint yourself with each of these objectives and their uses.

Stagemicroscope function

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C-mount and CS-mount are medium format screw mount lenses. Unlike board mount lenses, these lenses typically feature variable focus and aperture capabilities.

Mediumpower objective microscope function

Plus, your brain controls your entire nervous system. You would think it always reigns supreme, but sometimes it doesn’t. Once in a while your eyes are capable of tricking your brain into seeing things that aren’t really as they appear. Or are they? This is called an optical illusion.

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Oil Immersion Objective (100X): This longest objective is used for observing the detail of individual cells such as white blood cells, the cells involved in spermatogenesis, etc.  The lens must be used with a specially formulated oil that creates a bridge between the tip of the objective and the cover slip.  Since the refractive indices of air and this lens are different, the lens will not work without this special oil!

For example, the retinas in your eyes – the layer where visual images are formed – actually “sees” images upside down and backwards. Your brain flips them, so you see images right side up and reversed. Every single time. Yes, your brain is the original Photoshop genius.

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The fascination with optical illusions has spanned the globe for centuries. Greek philosophers like Plato described them as tricks played on us by our senses and our minds. By the 19th century, a famous cartoonist W.E. Hill embraced the phenomena of optical illusions by skillfully sketching a picture that was simultaneously two images. The figure you saw was determined by your perception. It was either a young girl or an old woman.

To use the lens, first make sure the specimen is in focus under the high power (40X) objective.  Next, move the high power objective out of position, place a small drop of oil on top of the cover slip above the specimen to be viewed and move the oil immersion lens into place.  Use the fine adjustment knob to bring the specimen into focus.  When you are finished, make sure to clean the lens and slide with lens paper.  Also, it is extremely important that this oil does not contact any of the other objectives.  If this should happen, clean the lenses immediately!

This type of illusion is the most researched by scientists and psychologists because it is the most complex type of trick the eye can play on the brain.  Unlike other optical illusions, these illusions rely on what the subconscious mind thinks and how it relates one object to another.  In other words, what you see is believed to give insight into the depths of your thinking. A cognitive optical illusion uncovers what your brain infers and understands about something that has not been explained.

Our Department of Ophthalmology is pleased to offer Optical Coherence Tomography (OCT), a highly sensitive diagnostic test used in the evaluation of conditions ...