Diffractiongrating in laser

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One of the characteristics of a microscope eyepiece is the microscopic Field of View commonly abbreviated FOV. The size of the FOV is expressed numerically in millimeters and is directly proportional to the combination of the eyepiece and the objective in use.

Grating in laserwavelength

The eyepiece, also referred to as an ocular, has a field stop as part of its design. The FOV is limited by this field stop and is defined as the diameter of the circle of light seen when looking into a microscope. The actual FOV is calculated by dividing the field number (FN) by the objective magnification. An eyepiece with FN=20 used with a 10X objective would have an FOV of 2.0 mm while an eyepiece where FN=26 would have an FOV of 2.6 mm. Widefield oculars have the advantage of showing a larger area of the sample at a given total magnification. Stereoscopic microscopes tend to offer a larger FOV even though their total magnification capabilities are less than compound microscope.

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