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Disable the option to Fit Video in Window when your video-camera resolution is lower than your computer-monitor resolution.

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Follow the onscreen prompt to switch to the next-highest power objective and select the corresponding software lens (some microscopes may select the software lens automatically)

Enable the option to Fit Video in Window when your video-camera resolution is higher than your computer-monitor resolution.

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Do not check the box to Enable parfocal because parfocal correction is controlled by the microscope (not the software). Refer to your microscope manual for more information on adjusting parfocality. On a Zeiss microscope, use the touch screen to access Home > Configuration > Focus > Parfocality Adjustment.

When camera resolution is lower than computer-monitor resolution, the entire video is displayed in the top left of the main Neurolucida window, with black regions bordering the bottom and right of the video. If enabled, Fit Video in Window will stretch the live-camera feed to fill the software window; uncheck the box to prevent this stretching.

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In the Select Lenses for Calibration window, verify that the lenses are listed in descending order, from highest to lowest power.

Parcentric and parfocal calibration compensate for the deviations from parfocality (focal plane) and parcentricity (collimation) that are normally encountered between different microscope objective lenses. They are both critical for maintaining position when changing magnification.

When camera resolution is higher than computer-monitor resolution, only the top left of the live-camera image will be visible in the main Neurolucida window. Select Fit video in Window to reduce the size of the live-camera image so that all of it is visible onscreen.

An alert is displayed to remind you that, for each lens, you need to click the exact same point on the grid, regardless of where it appears on the screen.

Once you've calibrated the lenses and verified calibration accuracy, you're ready to perform the parcentric/parfocal alignment.