Once you're set up the app presents to you the choice of objects, from galaxies and nebulae to stars and the Moon, that are currently high above the horizon and ideal for observing. The app has a built-in catalogue of about 5400 objects to choose from.

Figure 2(f) demonstrates autofluorescence in a thin section of Zamia (Zamia loddigesii) stem tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll. In the blue and green excitation regions, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 450 and 550 nanometers. Other endogenous fluorophores are excited by ultraviolet light with this filter combination. For the Zamia stem tissue illustrated above, note the presence of autofluorescence emission intensity in the blue, green, and red spectral regions, which is characteristic of the Nikon DAPI-FITC-Texas Red fluorescence filter combination.

Figure 2(a) illustrates the fluorescence emission profile from a thin section of rabbit lung tissue stained for nuclei, actin filaments, and glycoproteins with DAPI, Alexa Fluor 546-phalloidin, and Oregon Green 488 wheat germ agglutinin, respectively. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers, while the corresponding values for Oregon Green 488 are 496 and 524 nanometers. DAPI (4',6-diamidino-2-phenylindole) is an ultraviolet light-absorbing dye with an absorption maximum at 358 and an emission maximum in the blue region at 461 nanometers. Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen.

However, there are several changes. The new Nikon Electronic Eyepiece is very impressive. Branded with the camera-maker's logo, it's a lot clearer, more detailed, and much more involving than the eyepiece used on the original eVscope. Stars, nebulae and galaxies are presented against the jet black of space, and there's plenty of eye relief, which is good if you wear glasses. You can focus the eyepiece separately using a small knob.

There's a focus wheel on the underside of the eVscope. Although you can just manually focus on the Moon, it's best to use the Bahtinov mask. It's hidden in the lens cap. You put this plastic disc across the end of the telescope tube, point the eVscope at a bright star, then focus the telescope until you see three angled spikes of light. It's easy to do.

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The eVscope is so easy to set up. Once it's mounted on its tripod (complete with spirit level to get it flat) using two thumbscrews, you download the app, switch-on the telescope, tune-in to its WiFi Direct network, remove the lens cap and eyepiece cover, and press a button on the app to have indulge in 'Autonomous Field Detection'. All that means is that it cross-references what stars it sees with some built-in planetarium software, as well as using your phone's GPS to work out where it is and what it should be seeing. It works more quickly if you use the arrow keys on the app to gently point it south/south-east where the Milky Way is … the more stars, the better! Obviously, you need a clear night.

That eyepiece has a lot to work with. Inside the eVscope 2 is an all-new Sony IMX347 CMOS image sensor, which is capable of 3200x3200 pixel images. That's 7.7 megapixels, which is something of a leap from the 4.8 megapixel images offered by the original eVscope and the eVscope eQuinox. Crucially, the eVscope 2 also has a new, wider field of view, so as well as getting views of distant galaxies and nebulae you can also now get a full screen view of the Moon and the stunning Andromeda Galaxy. However, planets don't look good … aside from Jupiter.

Observe the fluorescence emission from a culture of normal human lung cells (MRC-5 line) stained with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation with blue emission).

FITCGFP

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View the emission intensity from a thin section of corn kernel (Zea mays) tissue using the Nikon DAPI-FITC-Texas Red filter set. For the corn kernel tissue illustrated in this section, note the presence of autofluorescence emission intensity in the blue, green, and red spectral regions (in some areas, mixed together to form white).

A culture of rat kangaroo kidney (PtK2 line) epithelial cells was immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin.

Fluorescence emission from a culture of albino Swiss mouse embryo cells (3T3 line) stained with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively, is illustrated in Figure 2(d). The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation with blue emission). Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen.

Available to buy now, following its launch in autumn 2021, the eVscope 2 costs €3,799 / £3,399 / $4,199, which is rather pricier than the debut product, the eVscope, which cost €2,999 / £2,599 / $2,999. Unistellar seems (wisely) to have scrapped its shipping charge, so there's free delivery in all major territories. There's a large tripod and a large, custom-made (and very comfortable) backpack included in the box.

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With a new, clearer Nikon eyepiece and a wider field of view, the Unistellar eVscope 2 smart 'scope can now be used to take sharper astro-photos of the Moon as well as distant galaxies, nebulae and stars clusters. However, its biggest attraction is that it deals expertly with light pollution.

Observe the fluorescence emission intensity from a culture of human osteosarcoma cells (U-2 line) stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the cellular mitochondrial network, cytoskeletal actin filaments, and nucleus, respectively.

A culture of rat kangaroo kidney epithelial (PtK2 line) cells was immunofluorescently labeled with primary anti-cytokeratin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy2. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers. Note the prominent staining of the cytokeratin network throughout the cytoplasm in these cells. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the mitochondrial network with MitoTracker Red CMXRos.

What is new to the eVscope 2 is more contrasty images with deeper blacks and richer star fields. We're talking here about better images – delivered as good quality PNG files – that are great for viewing on phones and tablets, and for sharing online. You can zoom in on them within the app up to 400x, but it's best not to go that far.

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to BODIPY FL.

FITCexcitation wavelength

The magic happens when you slew an object, which can take a while, though the eVscope is so accurate that we have no complaints. Once it has the object in its crosshairs you simply press the 'Enhanced Vision' button – a graphic of an eye – and it begins to take photos and stack them on top of each other. This is an advanced technique of astrophotographers and it helps produce incredible images even under light-polluted skies.

Fitc emission spectrumformula

A culture of bovine pulmonary artery epithelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to BODIPY FL is illustrated in Figure 2(b). The cell culture was simultaneously stained with Texas Red phalloidin and DAPI, which target the cytoskeletal actin network and DNA in the nucleus, respectively. The visible light absorption maximum of BODIPY FL is 505 nanometers and the emission maximum occurs at 513 nanometers, while the corresponding values for Texas Red are 595 and 620 nanometers.

You can watch either through the eyepiece (though don't touch the telescope) or at your phone or a tablet as the object in question gets brighter and more defined with time. Some faint nebulae are worth observing for a long time, others look great after just a few seconds. Either way, you can download them to devices and share them instantly.

A culture of Indian Muntjac deerskin fibroblast cells was stained with MitoTracker Red CMXRos and Alexa Fluor 488, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation and blue emission). Note the presence of high signal levels from the blue (DAPI) fluorophore, as well as the orange-red fluorescence exhibited by the tubular mitochondria and the green emission from actin filaments in the cytoplasm.

Examine the bright fluorescence emission intensity from a culture of bovine pulmonary artery epithelial cells stained with MitoTracker Red CMXRos, BODIPY FL conjugated to phallacidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nucleus, respectively. These combined probes all exhibit high signal levels with the Nikon DAPI-FITC-Texas Red triple band excitation filter combination.

Fluorescence emission intensity from a thin section of rabbit lung tissue stained for nuclei, actin filaments, and glycoproteins with DAPI, Alexa Fluor 546-phalloidin, and Oregon Green 488 wheat germ agglutinin, respectively.

With a new wider field of view and a few more pixels to play with, the eVscope 2 is a a souped-up smart scope that's great for polluted skies. This hugely likeable digital telescope serves as a great introduction – albeit a hugely expensive one – to the world of amateur astronomy and astrophotography, but without any of the hassle.

The DAPI-FITC-Texas Red triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and Texas Red with minimal crossover between adjacent emission bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles. The short wavelength signal channel of the filter set selects narrowly defined spectral regions for violet excitation and blue emission detection; a second, longer wavelength channel corresponds to blue excitation and green emission detection, and green excitation coupled to red emission constitutes the third band of the filter set. The short wavelength excitation transmission profile is deliberately reduced in size to provide better balance of the blue emission with the other fluorochrome signals, and to minimize photobleaching. This filter set is recommended when studying various combinations of fluorophores chosen from the following three groups: DAPI, blue fluorescent protein (BFP), or Hoechst dyes (violet excitation); FITC, green fluorescent protein (GFP), Cy2, or Alexa Fluor 488 (blue excitation); Texas Red or Alexa Fluor 594 (green excitation). The images presented in Figure 2 demonstrate the performance of this filter set with a variety of fluorescence probe combinations targeted at different intracellular locations.

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The eVscope seems, at first, to be identical to its predecessor. It has the same light grey tube and comes propped-up on a large tripod with three-section legs. Each is held steady by large, solid cams.

FITCcolor

It's one of a trio of improvements. With a new wider field of view and a higher resolution Sony sensor inside, the eVscope 2 is surely the most refined digital telescope yet. It's one of the best telescopes we've tried, and because it's so incredibly easy to use, it's also one of the best beginner telescopes (albeit one of the most expensive). Read on for our full Unistellar eVscope 2 digital telescope review.

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells.

The ambient LED glow over every city on Earth is an absolute nightmare for amateur astronomers, but somehow the eVscope's filters and stacking tech helps get over it. Being able to image distant nebulae while not half a mile from a busy city centre is a thing of wonder. However, light pollution does make a difference. For example, during nights with a bright Moon it's best to point the eVscope at targets on the other side of the night sky, where it's much darker (after you're snapped the Moon, of course). It's also why the eVscope ships with that excellent backpack; so you can easily take it to truly dark rural skies. Do that and it can see objects down to a limiting magnitude of 18.

John D. Griffin, Nathan S. Claxton, Matthew J. Parry-Hill, Thomas J. Fellers, Kimberly M. Vogt, Ian D. Johnson, Shannon H. Neaves, Omar Alvarado, Lionel Parsons, Jr., Michael A. Sodders, Richard L. Ludlow, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

FITClaser

Autofluorescence emission intensity from a thin section of Zamia (Zamia loddigesii) stem tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll.

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The Unistellar eVscope 2 is a digital telescope from the Maseille-based company. What exactly is a digital telescope? It's a regular telescope that's fully motorised and controlled exclusively via an app on a smartphone or tablet. However, as well as getting telescopes to slew to various deep sky targets just at the touch of a button, a digital telescope is fitted with a camera sensor, so in that way it's as much about astrophotography as amateur astronomy.

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FITCvs GFP

Fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) that were immunofluorescently labeled with primary anti-human golgin-97 mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein (through the isothiocyanate, FITC).

A culture of bovine pulmonary artery endothelial (BPAE line) cells was immunofluorescently labeled with primary anti-vinculin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3. The visible light absorption maximum of Cy3 is 552 nanometers and the emission maximum occurs at 570 nanometers. Note the prominent staining of the cellular attachment network in the central portion and periphery of these cells. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin.

Examine a thin section of rat epidermal skin tissue stained for DNA and glycoproteins with DAPI and tetramethylrhodamine (TMR) conjugated to wheat germ agglutinin, respectively. The visible light absorption maximum of TMR is 550 nanometers and the corresponding emission maximum occurs at 573 nanometers. In addition, the specimen was simultaneously labeled with Alexa Fluor 488 conjugated to phalloidin (targeting the actin network; green emission). Note the presence of both overlapping and discrete signals from all three of these fluorophores.

Observe the fluorescence emission from a culture of normal African green monkey kidney cells (CV-1 line) stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nucleus, respectively. Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen, as well as the unusual morphology of these cells.

Three emission (barrier) filter bandpass regions enable detection of blue, green, and red emission from the three fluorochromes simultaneously. Sharp wavelength transitions between transmission and reflection bands for each filter, and application of a dichromatic mirror having multiple bandpass transmission regions chosen to be complementary to the emission and excitation wavelengths, permit triple-band signal detection with minimal crossover (spectral bleed-through), interference, and noise.

FITCstructure

HeLa carcinoma cells were transfected with an EGFP-peroxisomal targeting signal 1 (PTS1) fusion protein and stained with Alexa Fluor 546-phalloidin. These fluorescent probes target the peroxisomes and cytoskeletal actin filament network, respectively. The visible light absorption maximum of the EGFP-PTS1 chimera is 488 nanometers and the emission maximum occurs at 507 nanometers. In addition, the specimen was simultaneously stained with Hoechst 33258 (targeting the DNA in the nucleus; blue emission). Note the presence of signal from the blue fluorophore, as well as the bright orange-red fluorescence exhibited by the cytoskeletal actin filaments and the intense green emission from peroxisomes in the cytoplasm.

Fluorescence emission intensity from a culture of albino Swiss mouse embryo cells (3T3line) stained with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively.

Yes, the eVscope is higher resolution than its forbear, but in part those extra pixels help to make up for its slightly wider field of view. So while the 7.7 megapixels are very welcome, you're not going to be wowed by ultra HD views.

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Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins.

Presented in Figure 2(c) is a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the presence of signal from the blue fluorophore (Alexa Fluor 350), as well as the bright green fluorescence of nuclei in the tissue specimen due to SYTOX Green, and the orange-red fluorescence (Alexa Fluor 568) from actin filaments in the brush border.

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A thin section of rabbit bone marrow tissue was stained for DNA and glycoproteins with DAPI and Oregon Green 488 conjugated to wheat germ agglutinin, respectively. The visible light absorption maximum of Oregon Green is 496 nanometers and the emission maximum occurs at 524 nanometers. In addition, the specimen was simultaneously labeled with Alexa Fluor 546 conjugated to phalloidin (targeting the actin network; yellow-orange emission).

Anna Scordato and Stanley Schwartz - Bioscience Department, Nikon Instruments, Inc., 1300 Walt Whitman Road, Melville, New York, 11747.

Examine the fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI. Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen.

Canine kidney epithelial cells (Madin-Darby; MDCK line) that were immunofluorescently labeled with primary anti-human golgin-97 mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein (through the isothiocyanate, FITC) are demonstrated in Figure 2(e). The visible light absorption maximum of FITC is 494 nanometers and the emission maximum occurs at 518 nanometers in the blue-green region of the spectrum. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and Alexa Fluor 568 conjugated to phalloidin (targeting the cytoskeletal actin network; orange-red emission). Anti-human golgin-97 antibodies recognize a 97-kiloDalton protein termed golgin-97, which is a member of the granin protein family and a peripheral membrane protein localized on the cytoplasmic face of the Golgi apparatus. Note the presence of green mitochondria in this specimen.

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The Unistellar eVscope 2 digital telescope has a new eyepiece, a wider field of view and puts higher-res views of galaxies, nebulae and stars clusters on your phone or tablet. Here's our review

Having created the first digital telescopes that use a camera sensor to deliver deep sky images to an app on a smartphone, Unistellar abandoned the idea of physically looking through a telescope with the launch of its eVscope eQuinox telescope in March 2021. However, not only does the eVscope 2, which launched in autumn 2021, see the return of an eyepiece, but it's made by no less a company than Nikon.

FITCfluorescence excitationemission

Ultraviolet and visible transmission spectral profiles for the Nikon DAPI-FITC-Texas Red filter combination are illustrated below in Figure 1. This filter set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and Texas Red probes when applied in combination (triple) staining techniques. The DAPI-FITC-Texas Red set incorporates an excitation filter with narrow bandpass windows in the violet (395 to 410 nanometers), blue (490 to 505 nanometers), and green (560 to 580 nanometers) spectral regions.

Examine the fluorescence emission intensity from a culture of human lung cells (MRC-5 line) stained with wheat germ agglutinin conjugated to Texas Red, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular Golgi network, cytoskeletal actin filaments, and nucleus, respectively. The visible light absorption maximum of Texas Red is 596 nanometers and the emission maximum occurs at 615 nanometers. Note the presence of significant signal levels from all three of the fluorophores employed to stain the specimen, and the high degree of localization for wheat germ agglutinin to the Golgi apparatus.

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