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The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C:

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Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei.

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A common variant is Lillie's trichrome. It is often errorneously called Masson's trichrome. It differs in the dyes used, their concentrations, and the immersion times.

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Masson's trichrome is a three-color staining protocol used in histology. The recipes evolved from the original Masson's formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue.

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This produces a magnified, virtual image. With a microscope, a relay lens system replaces the single lens; an objective and an eyepiece work in tandem to ...