Microscopyu

The Olympus FluoView FVMPE-RS multiphoton microscope allows fluorescence imaging at higher depths within the specimen. The upright microscope provides space for large samples and a high degree of motorization. With its ultra-stable arch-like structure, the gantry microscope system offers a high degree of flexibility to suit different samples. This is ideal for in vivo observation requiring maximum space. To facilitate simultaneous two-color excitation, it is equipped with Spectra-Physics Insight X3 tunable ultrafast lasers, having a dual output (I) tunable laser output from 690nm to 1300 nm (II) Fixed laser line at 1045. In addition to the high NA 10X and 25 X objectives, the microscopeis equipped with a computer-controlled correction collar system that can automatically adjust to compensate for spherical aberration during deep observation of thick samples. There are 4 non-descanned detectors on the microscope, 2 PMTs and 2 GAAsP detectors. The motorized stage can be used to image from slides, dishes and small animals. The Core also has an E-Z Anesthesia machine to help intra-vital imaging experiments.

Typically, a production line includes multiple washing and rinsing baths, including ultrasonic cleaning to remove any traces of surface contaminants. This is followed by air drying and heating of the lenses in special ovens to further remove unwanted moisture and gases from the lens surface.

When cleaning AR-coated lenses, use only products that your optician recommends. Lens cleaners with harsh chemicals may damage the anti-reflective coating.

Most premium anti-reflective coatings include a "hydrophobic" surface layer that prevents water spots from forming and makes the lenses easier to clean. Some AR coatings also include an "oleophobic" surface layer that repels skin oils and makes it easier to remove smudges from the lenses.

One type of optical coating is an anti-reflective coating, which reduces unwanted reflections from surfaces, and is commonly used on spectacle and camera lenses ...

Feb 5, 2009 — "IR cut" and "hot mirror" seem to be used to mean the same thing -- decreasing IR that would get to the sensor. But there are many ways to do ...

Some eyeglass lenses have factory-applied AR coating on both lens surfaces. Other lenses, particularly progressive lenses and other multifocal lenses (i.e., bifocals and trifocals), have the coating applied after the lenses have been customized to your eyeglass prescription by an optical lab.

In addition, the microscope is equipped with an Inner Focus Articulating Nosepiece that can be mounted on the microscope to provide more degrees of freedom using a moveable arm while also facilitating rapid axial focusing with no mechanical movement. The software aids in acquiring large mosaic tiles to image large regions. Together with all these features, the microscope can be used for high-sensitivity, high-resolution deep tissue imaging, intra-vital imaging, optogenetics, electrophysiology, label-free imaging (SHG, THG), etc.

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While the lens racks are rotating in the coating chamber, a power source within the machine focuses a beam of electrons onto a small crucible that contains a series of metal oxides in separate compartments. When bombarded by the beam of this electron "gun" in succession, the metal oxides are transformed into vapors that fill the coating chamber and adhere to the lenses in a specific order to form a precise multilayer AR coating.

Both benefits are due to AR coating's ability to eliminate reflections of light from the front and back surface of eyeglass lenses.

Anti-reflective coatings are incredibly thin. The entire multilayer AR coating stack generally is only about 0.2 to 0.3 microns thick, or about 0.02 percent (two one-hundredths of 1 percent) of the thickness of a standard eyeglass lens.

Anti-reflective coatings (also called AR coating or anti-glare coating) improve both your vision through your lenses and the appearance of your eyeglasses.

Multiphotonmicroscopy

Aug 9, 2017 — It merely means that the system is limited by the diffraction characteristics of an aperture with the same central obstruction. The aberrations ...

Today's modern anti-reflective coatings can virtually eliminate the reflection of light from eyeglass lenses, allowing 99.5 percent of available light to pass through the lenses and enter the eye for good vision.

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Two-photon microscopy

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The first step in the AR coating process is to meticulously clean the lenses and inspect them for visible and microscopic surface defects. Even a tiny smudge, piece of lint or hairline scratch on a lens during the coating process can cause a defective AR coating.

The Olympus FluoView FV1000MPE is a multi-photon laser scanning microscope that allows fluorescence imaging deep within specimens. It has a Coherent Chameleon Vision II laser, tunable from 680nm to 1080nm, as well as a 473nm laser diode for single photon excitation. It has four external (non-descan) detectors, as well as three confocal PMTs and one transmitted light detector. It is capable of timelapse and FRET imaging experiments. The motorized stage contains adapters for different dishes, slides, and small animals to accommodate a wide range of experimental setups, and is also capable of automated montage acquisition and stitching to generate high resolution images of large areas. Dipping lenses allow imaging directly into specimens in media.

Nov 13, 2015 — Negative eyepieces have two lenses: the upper lens, which is closest to the observer's eye, is called the eye-lens and the lower lens (beneath ...

The Olympus FluoView FV1000MPE is a multi-photon laser scanning microscope that allows fluorescence imaging deep within specimens. It has a Coherent Chameleon Vision II laser, tunable from 680nm to 1080nm, as well as a 473nm laser diode for single photon excitation. It has four external (non-descan) detectors, as well as three confocal PMTs and one transmitted light detector. It is capable of timelapse and FRET imaging experiments. The motorized stage contains adapters for different dishes, slides, and small animals to accommodate a wide range of experimental setups, and is also capable of automated montage acquisition and stitching to generate high resolution images of large areas. Dipping lenses allow imaging directly into specimens in media. User ProtocolMosaic Protocol

The lenses are then loaded into special metal racks with spring-loaded openings so the lenses are held securely but with virtually all lens surfaces exposed for the coating application. The racks are then loaded into the coating chamber. The door of the chamber is sealed, and the air is pumped out of the chamber to create a vacuum.

Each AR coating manufacturer has its own proprietary formula, but generally all anti-reflective coatings consist of multiple microscopic layers of metallic oxides of alternating high and low index of refraction. Depending on the AR coating formula, most lenses with anti-reflective coating have a very faint residual color, usually green or blue, that is characteristic of that particular brand of coating.

The energy increase or decrease is related to the vibrational energy levels in the ground electronic state of the molecule. The observed Raman shift of the ...

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The Olympus FluoView FVMPE-RS multiphoton microscope allows fluorescence imaging at higher depths within the specimen. The upright microscope provides space for large samples and a high degree of motorization. With its ultra-stable arch-like structure, the gantry microscope system offers a high degree of flexibility to suit different samples. This is ideal for in vivo observation requiring maximum space. To facilitate simultaneous two-color excitation, it is equipped with Spectra-Physics Insight X3 tunable ultrafast lasers, having a dual output (I) tunable laser output from 690nm to 1300 nm (II) Fixed laser line at 1045. In addition to the high NA 10X and 25 X objectives, the microscopeis equipped with a computer-controlled correction collar system that can automatically adjust to compensate for spherical aberration during deep observation of thick samples. There are 4 non-descanned detectors on the microscope, 2 PMTs and 2 GAAsP detectors. The motorized stage can be used to image from slides, dishes and small animals. The Core also has an E-Z Anesthesia machine to help intra-vital imaging experiments. In addition, the microscope is equipped with an Inner Focus Articulating Nosepiece that can be mounted on the microscope to provide more degrees of freedom using a moveable arm while also facilitating rapid axial focusing with no mechanical movement. The software aids in acquiring large mosaic tiles to image large regions. Together with all these features, the microscope can be used for high-sensitivity, high-resolution deep tissue imaging, intra-vital imaging, optogenetics, electrophysiology, label-free imaging (SHG, THG), etc. User Protocol

Also, don't attempt to clean AR-coated lenses without wetting them first. Using a dry cloth on a dry lens can cause lens scratches. And because anti-reflective coating eliminates light reflections that can mask lens surface defects, fine scratches often are more visible on AR-coated lenses than on uncoated lenses.

The visual benefits of lenses with anti-reflective coating include sharper vision with less glare when driving at night and greater comfort during prolonged computer use (compared with wearing eyeglass lenses without AR coating).

Applying anti-reflective coatings to eyeglass lenses is a highly technical process involving vacuum deposition technology.

Deep tissue two-photon microscopy

The technology uses fiber-optic cable, which amazingly can send data as fast as about 70% the speed of light. In addition, fiber-optic cables are not as ...

By eliminating reflections, AR coatings also makes your eyeglass lenses look nearly invisible so people can see your eyes and facial expressions more clearly. Anti-reflective glasses also are more attractive, so you can look your best in all lighting conditions.

AR coatings are especially beneficial when used on high-index lenses, which reflect more light than regular plastic lenses. Generally, the higher the index of refraction of the lens material, the more light that will be reflected from the surface of the lenses.

For example, regular plastic lenses reflect roughly 8 percent of light hitting the lenses, so only 92 percent of available light enters the eye for vision. High index plastic lenses can reflect up to 50 percent more light than regular plastic lenses (approximately 12 percent of available light), so even less light is available to the eye for vision. This can be particularly troublesome in low-light conditions, such as when driving at night.

In practice, Raman spectra are plotted as Raman shift. Raman shift is the difference between the peak energies and the excitation laser energy. This allows ...