Ocularlens magnification

Field of View: This is the maximum area visible when looking through the microscope eyepiece.       - When magnification increases the field of view        decreases      - When magnification decreases the field of view        increases

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Ocular objectivevs magnification

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3. Objective lens: The magnification of objective lenses typically ranges from 4x to 100x. Each lens magnification is designated on the metal casing of the lens.

Ocular objectiveexample

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9. Mechanical stage controls: These controls permit the slide, once clipped into the stage, to be maneuvered in the x-y axis.

Objectivelens microscope function

Inverted Image: When looking at a specimen through the microscope the image viewed through the eyepiece will be inverted, flipped upside down.

High powerobjectivelens

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Example: An individual is viewing a slide under the microscope using the 40x objective.  Total magnification = 10x (ocular lens magnification) x 40x (objective lens magnification) = 400x total magnification

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4. Coarse focus: Knob used for large-scale movements with the 4x objective lens. Rotation of this knob will move the stage closer or further away from the objective lens to permit focusing.

Objectivelens

1. Ocular lens (eyepiece): Each eyepiece contains a lens called the occular lens. The ocular lens typically has a magnification of 10x.

Objectivelens magnification

Ocular objectivemicroscope

2. Revolving nose piece (to hold multiple objective lenses): The nose piece allows for the user to change which objective lens is in place.

5. Fine focus: Knob used for small-scale movements with the 10x, 40x or 100x objective lens. Rotation of this knob will move the stage closer or further away from the objective lens to permit focusing.

10. Light intensity adjustor: Another knob that some microscopes will have located on their base to control light intenstity.

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8. Condenser and Diaphragm: The condenser is located below the stage. Light from the illuminator passes through the condenser, which focuses all the light rays onto the specimen to maximize illumination. The diaphragm (also referred to as the iris) is located between the condenser and the specimen. The diaphragm allows the user to adjust the amount of light reaching the speimen.

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