Be able to design, to first order, such optical systems with simple mathematical and graphical techniques.

Working distanceof lens

It really depends on your sample. A mostly flat sample can afford a short working distance and good resolution. A sample with a lot of differing heights or peaks and valleys will benefit from a long working distance objective so as not to crash into neighbouring peaks.

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a device for illuminating, as a light source with a lens or mirror for concentrating light. a person who paints manuscripts, books, etc., with designs in color, ...

Lenses with a focal length shorter than this are called wide-angle lenses, and those with a longer focal length are called telephoto lenses. However, there are ...

Definitions: Focal Length and Field of View ... The focal length of a lens is the distance in mm from the optical center of the lens to the focal point. In a ...

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Parfocal length

At some point we all have This is where the concept of Working Distance comes in. You will see Working Distance (WD) often listed as a spec in microscope or objective brochures. This spec is important as it tells you the distance between the microscope objective lens and your focused sample.

One other benefit of longer working distance is you have less out of focus areas in a thicker sample. This is known as Depth of Field.

Working distancemicroscope

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Focal Length. opening angle. Using sensor size and focal length Using working distance and object size (FOV). Focal Length. depth of field. Calculate depth of ...